##################################
# NO CUT ENZYMES (   62 enzymes) #
##################################

Acc65I      AgeI        AhdI        AocI        Aor51HI     
ApaI        AscI        Asp718I     AspEI       AviII       
AvrII       BbeI        BlnI        BsaBI       BsiEI       
BsiWI       BspDI       BssSI       BstBI       Bsu36I      
ClaI        CpoI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      FseI        
FspI        HgaI        HpaI        KasI        KpnI        
KspI        MamI        MluI        NaeI        NarI        
NgoMIV      NheI        NotI        NruI        NspV        
PinAI       PmeI        PshAI       PvuI        RsrII       
SacII       SalI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SpeI        SwaI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AatII        1792  GACGTC               GACGT^C
AccIII       2241  TCCGGA               T^CCGGA
AclI         6342  AACGTT               AA^CGTT
AcyI         1792  GACGTC               G[GA]^CG[CT]C
AseI         7364  ATTAAT               AT^TAAT
AsnI         7364  ATTAAT               AT^TAAT
BalI         1285  TGGCCA               TGG^CCA
BamHI        2085  GGATCC               G^GATCC
BcgI         1742  CGACTCTACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        3941  GTATCC               GTATCC(6/5)
BclI         6587  TGATCA               T^GATCA
BglI         3544  GCCTGAAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         1029  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1029  GCTCAGC              GC^T[ACGT]AGC
BsaHI        1792  GACGTC               G[GA]^CG[CT]C
BsaWI        2241  TCCGGA               [AT]^CCGG[AT]
BseAI        2241  TCCGGA               T^CCGGA
BsmBI        1790  GAGACG               CGTCTC(1/5)
BspEI        2241  TCCGGA               T^CCGGA
BsrFI        4741  GCCGGT               [GA]^CCGG[CT]
BssHII        124  GCGCGC               G^CGCGC
Bst1107I     6217  GTATAC               GTA^TAC
BstAPI       6667  GCATTGCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       3889  GGTAACC              G^GT[ACGT]ACC
BstPI        3889  GGTAACC              G^GT[ACGT]ACC
BstZ17I      6217  GTATAC               GTA^TAC
CelII        1029  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       4741  GCCGGT               [GA]^CCGG[CT]
DraII          60  AGGACCC              [GA]G^G[ACGT]CC[CT]
DsaI         3436  CCATGG               C^C[GA][CT]GG
EaeI         1285  TGGCCA               [CT]^GGCC[GA]
EcoO109I       60  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO65I      3889  GGTAACC              G^GT[ACGT]ACC
EcoRV         698  GATATC               GAT^ATC
FbaI         6587  TGATCA               T^GATCA
HaeII        1091  GGCGCT               [GA]GCGC^[CT]
Hin1I        1792  GACGTC               G[GA]^CG[CT]C
HindIII      4488  AAGCTT               A^AGCTT
MluNI        1285  TGGCCA               TGG^CCA
MroI         2241  TCCGGA               T^CCGGA
MscI         1285  TGGCCA               TGG^CCA
NcoI         3436  CCATGG               C^CATGG
PacI         7108  TTAATTAA             TTAAT^TAA
PaeR7I       5113  CTCGAG               C^TCGAG
PflMI        7271  CCAATGCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI          60  AGGACCC              [GA]G^G[AT]CC[CT]
PshBI        7364  ATTAAT               AT^TAAT
Psp1406I     6342  AACGTT               AA^CGTT
SbfI         2509  CCTGCAGG             CCTGCA^GG
SnaBI        4465  TACGTA               TAC^GTA
SphI         4155  GCATGC               GCATG^C
Sse8387I     2509  CCTGCAGG             CCTGCA^GG
StuI         2506  AGGCCT               AGG^CCT
Van91I       7271  CCAATGCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         5156  TCTAGA               T^CTAGA
XcmI         3609  CCAATTCCAGTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         5113  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

Alw44I       1354  GTGCAC               G^TGCAC
             6674  GTGCAC              
ApaLI        1354  GTGCAC               G^TGCAC
             6674  GTGCAC              
Asp700I      1047  GAAGGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3468  GAATCGTTTC          
AvaI         5113  CTCGAG               C^[CT]CG[GA]G
             6716  CTCGGG              
BbrPI        3392  CACGTG               CAC^GTG
             4294  CACGTG              
BbvCI          56  GCTGAGG              CCTCAGC(-5/-2)
             5480  GCTGAGG             
BcnI           11  CCGGG                CC^[GC]GG
               52  CCGGG               
BsaI           14  GGTCTC               GGTCTC(1/5)
             2720  GGTCTC              
BseRI        2370  CTCCTC               GAGGAG(10/8)
              843  GAGGAG              
BsoBI        5113  CTCGAG               C^[CT]CG[GA]G
             6716  CTCGGG              
BspHI        4773  TCATGA               T^CATGA
             5345  TCATGA              
BspMI          67  ACCTGC               ACCTGC(4/8)
              580  ACCTGC              
BsrBI         118  CCGCTC               CCGCTC(-3/-3)
             3406  CCGCTC              
EcoNI        3566  CCTCTCTAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             7508  CCTTAAGTAGG         
EcoRI         919  GAATTC               G^AATTC
             6941  GAATTC              
EcoT22I      4527  ATGCAT               ATGCA^T
             7274  ATGCAT              
FauI           19  CCCGC                CCCGC(4/6)
             1364  GCGGG               
HincII        710  GTTGAC               GT[CT]^[GA]AC
             5070  GTCAAC              
HindII        710  GTTGAC               GT[CT]^[GA]AC
             5070  GTCAAC              
MfeI         1589  CAATTG               C^AATTG
             5913  CAATTG              
MunI         1589  CAATTG               C^AATTG
             5913  CAATTG              
NciI           11  CCGGG                CC^[GC]GG
               52  CCGGG               
NsiI         4527  ATGCAT               ATGCA^T
             7274  ATGCAT              
PmaCI        3392  CACGTG               CAC^GTG
             4294  CACGTG              
PmlI         3392  CACGTG               CAC^GTG
             4294  CACGTG              
PvuII         218  CAGCTG               CAG^CTG
             7213  CAGCTG              
RcaI         4773  TCATGA               T^CATGA
             5345  TCATGA              
SapI          306  GAAGAGC              GCTCTTC(1/4)
             5569  GAAGAGC             
SexAI        3583  ACCAGGT              A^CC[AT]GGT
             3783  ACCTGGT             
XmnI         1047  GAAGGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3468  GAATCGTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  109 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AflII       AflIII      AluI        AlwI        AlwNI       
ApoI        AspHI       AspI        AvaII       BanI        
BanII       BbsI        BbvI        BfaI        BfrI        
BglII       BmyI        BpmI        BpuAI       BsaAI       
BsaJI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    Bsp1407I    
BspLU11I    BsrDI       BsrGI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        DraIII      EarI        EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NdeI        NdeII       NlaIII      NlaIV       NspI        
PflFI       PleI        PstI        RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspBI       SspI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     XhoII