################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## Acc65I AgeI AhdI AocI Aor51HI ApaI AscI Asp718I AspEI AviII AvrII BbeI BlnI BsaBI BsiEI BsiWI BspDI BssSI BstBI Bsu36I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I FseI FspI HgaI HpaI KasI KpnI KspI MamI MluI NaeI NarI NgoMIV NheI NotI NruI NspV PinAI PmeI PshAI PvuI RsrII SacII SalI ScaI SfiI SfoI SfuI SgrAI SmaI SpeI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AatII 1792 GACGTC GACGT^C AccIII 2241 TCCGGA T^CCGGA AclI 6342 AACGTT AA^CGTT AcyI 1792 GACGTC G[GA]^CG[CT]C AseI 7364 ATTAAT AT^TAAT AsnI 7364 ATTAAT AT^TAAT BalI 1285 TGGCCA TGG^CCA BamHI 2085 GGATCC G^GATCC BcgI 1742 CGACTCTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3941 GTATCC GTATCC(6/5) BclI 6587 TGATCA T^GATCA BglI 3544 GCCTGAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1029 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1029 GCTCAGC GC^T[ACGT]AGC BsaHI 1792 GACGTC G[GA]^CG[CT]C BsaWI 2241 TCCGGA [AT]^CCGG[AT] BseAI 2241 TCCGGA T^CCGGA BsmBI 1790 GAGACG CGTCTC(1/5) BspEI 2241 TCCGGA T^CCGGA BsrFI 4741 GCCGGT [GA]^CCGG[CT] BssHII 124 GCGCGC G^CGCGC Bst1107I 6217 GTATAC GTA^TAC BstAPI 6667 GCATTGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 3889 GGTAACC G^GT[ACGT]ACC BstPI 3889 GGTAACC G^GT[ACGT]ACC BstZ17I 6217 GTATAC GTA^TAC CelII 1029 GCTCAGC GC^T[ACGT]AGC Cfr10I 4741 GCCGGT [GA]^CCGG[CT] DraII 60 AGGACCC [GA]G^G[ACGT]CC[CT] DsaI 3436 CCATGG C^C[GA][CT]GG EaeI 1285 TGGCCA [CT]^GGCC[GA] EcoO109I 60 AGGACCC [GA]G^G[ACGT]CC[CT] EcoO65I 3889 GGTAACC G^GT[ACGT]ACC EcoRV 698 GATATC GAT^ATC FbaI 6587 TGATCA T^GATCA HaeII 1091 GGCGCT [GA]GCGC^[CT] Hin1I 1792 GACGTC G[GA]^CG[CT]C HindIII 4488 AAGCTT A^AGCTT MluNI 1285 TGGCCA TGG^CCA MroI 2241 TCCGGA T^CCGGA MscI 1285 TGGCCA TGG^CCA NcoI 3436 CCATGG C^CATGG PacI 7108 TTAATTAA TTAAT^TAA PaeR7I 5113 CTCGAG C^TCGAG PflMI 7271 CCAATGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 60 AGGACCC [GA]G^G[AT]CC[CT] PshBI 7364 ATTAAT AT^TAAT Psp1406I 6342 AACGTT AA^CGTT SbfI 2509 CCTGCAGG CCTGCA^GG SnaBI 4465 TACGTA TAC^GTA SphI 4155 GCATGC GCATG^C Sse8387I 2509 CCTGCAGG CCTGCA^GG StuI 2506 AGGCCT AGG^CCT Van91I 7271 CCAATGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 5156 TCTAGA T^CTAGA XcmI 3609 CCAATTCCAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 5113 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### Alw44I 1354 GTGCAC G^TGCAC 6674 GTGCAC ApaLI 1354 GTGCAC G^TGCAC 6674 GTGCAC Asp700I 1047 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3468 GAATCGTTTC AvaI 5113 CTCGAG C^[CT]CG[GA]G 6716 CTCGGG BbrPI 3392 CACGTG CAC^GTG 4294 CACGTG BbvCI 56 GCTGAGG CCTCAGC(-5/-2) 5480 GCTGAGG BcnI 11 CCGGG CC^[GC]GG 52 CCGGG BsaI 14 GGTCTC GGTCTC(1/5) 2720 GGTCTC BseRI 2370 CTCCTC GAGGAG(10/8) 843 GAGGAG BsoBI 5113 CTCGAG C^[CT]CG[GA]G 6716 CTCGGG BspHI 4773 TCATGA T^CATGA 5345 TCATGA BspMI 67 ACCTGC ACCTGC(4/8) 580 ACCTGC BsrBI 118 CCGCTC CCGCTC(-3/-3) 3406 CCGCTC EcoNI 3566 CCTCTCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 7508 CCTTAAGTAGG EcoRI 919 GAATTC G^AATTC 6941 GAATTC EcoT22I 4527 ATGCAT ATGCA^T 7274 ATGCAT FauI 19 CCCGC CCCGC(4/6) 1364 GCGGG HincII 710 GTTGAC GT[CT]^[GA]AC 5070 GTCAAC HindII 710 GTTGAC GT[CT]^[GA]AC 5070 GTCAAC MfeI 1589 CAATTG C^AATTG 5913 CAATTG MunI 1589 CAATTG C^AATTG 5913 CAATTG NciI 11 CCGGG CC^[GC]GG 52 CCGGG NsiI 4527 ATGCAT ATGCA^T 7274 ATGCAT PmaCI 3392 CACGTG CAC^GTG 4294 CACGTG PmlI 3392 CACGTG CAC^GTG 4294 CACGTG PvuII 218 CAGCTG CAG^CTG 7213 CAGCTG RcaI 4773 TCATGA T^CATGA 5345 TCATGA SapI 306 GAAGAGC GCTCTTC(1/4) 5569 GAAGAGC SexAI 3583 ACCAGGT A^CC[AT]GGT 3783 ACCTGGT XmnI 1047 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3468 GAATCGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflII AflIII AluI AlwI AlwNI ApoI AspHI AspI AvaII BanI BanII BbsI BbvI BfaI BfrI BglII BmyI BpmI BpuAI BsaAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspLU11I BsrDI BsrGI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraIII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeI NdeII NlaIII NlaIV NspI PflFI PleI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoII