##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
Acc65I AgeI AhdI AocI Aor51HI
ApaI AscI Asp718I AspEI AviII
AvrII BbeI BlnI BsaBI BsiEI
BsiWI BspDI BssSI BstBI Bsu36I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I FseI
FspI HgaI HpaI KasI KpnI
KspI MamI MluI NaeI NarI
NgoMIV NheI NotI NruI NspV
PinAI PmeI PshAI PvuI RsrII
SacII SalI ScaI SfiI SfoI
SfuI SgrAI SmaI SpeI SwaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 1792 GACGTC GACGT^C
AccIII 2241 TCCGGA T^CCGGA
AclI 6342 AACGTT AA^CGTT
AcyI 1792 GACGTC G[GA]^CG[CT]C
AseI 7364 ATTAAT AT^TAAT
AsnI 7364 ATTAAT AT^TAAT
BalI 1285 TGGCCA TGG^CCA
BamHI 2085 GGATCC G^GATCC
BcgI 1742 CGACTCTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3941 GTATCC GTATCC(6/5)
BclI 6587 TGATCA T^GATCA
BglI 3544 GCCTGAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1029 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1029 GCTCAGC GC^T[ACGT]AGC
BsaHI 1792 GACGTC G[GA]^CG[CT]C
BsaWI 2241 TCCGGA [AT]^CCGG[AT]
BseAI 2241 TCCGGA T^CCGGA
BsmBI 1790 GAGACG CGTCTC(1/5)
BspEI 2241 TCCGGA T^CCGGA
BsrFI 4741 GCCGGT [GA]^CCGG[CT]
BssHII 124 GCGCGC G^CGCGC
Bst1107I 6217 GTATAC GTA^TAC
BstAPI 6667 GCATTGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3889 GGTAACC G^GT[ACGT]ACC
BstPI 3889 GGTAACC G^GT[ACGT]ACC
BstZ17I 6217 GTATAC GTA^TAC
CelII 1029 GCTCAGC GC^T[ACGT]AGC
Cfr10I 4741 GCCGGT [GA]^CCGG[CT]
DraII 60 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 3436 CCATGG C^C[GA][CT]GG
EaeI 1285 TGGCCA [CT]^GGCC[GA]
EcoO109I 60 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 3889 GGTAACC G^GT[ACGT]ACC
EcoRV 698 GATATC GAT^ATC
FbaI 6587 TGATCA T^GATCA
HaeII 1091 GGCGCT [GA]GCGC^[CT]
Hin1I 1792 GACGTC G[GA]^CG[CT]C
HindIII 4488 AAGCTT A^AGCTT
MluNI 1285 TGGCCA TGG^CCA
MroI 2241 TCCGGA T^CCGGA
MscI 1285 TGGCCA TGG^CCA
NcoI 3436 CCATGG C^CATGG
PacI 7108 TTAATTAA TTAAT^TAA
PaeR7I 5113 CTCGAG C^TCGAG
PflMI 7271 CCAATGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 60 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 7364 ATTAAT AT^TAAT
Psp1406I 6342 AACGTT AA^CGTT
SbfI 2509 CCTGCAGG CCTGCA^GG
SnaBI 4465 TACGTA TAC^GTA
SphI 4155 GCATGC GCATG^C
Sse8387I 2509 CCTGCAGG CCTGCA^GG
StuI 2506 AGGCCT AGG^CCT
Van91I 7271 CCAATGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 5156 TCTAGA T^CTAGA
XcmI 3609 CCAATTCCAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 5113 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Alw44I 1354 GTGCAC G^TGCAC
6674 GTGCAC
ApaLI 1354 GTGCAC G^TGCAC
6674 GTGCAC
Asp700I 1047 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3468 GAATCGTTTC
AvaI 5113 CTCGAG C^[CT]CG[GA]G
6716 CTCGGG
BbrPI 3392 CACGTG CAC^GTG
4294 CACGTG
BbvCI 56 GCTGAGG CCTCAGC(-5/-2)
5480 GCTGAGG
BcnI 11 CCGGG CC^[GC]GG
52 CCGGG
BsaI 14 GGTCTC GGTCTC(1/5)
2720 GGTCTC
BseRI 2370 CTCCTC GAGGAG(10/8)
843 GAGGAG
BsoBI 5113 CTCGAG C^[CT]CG[GA]G
6716 CTCGGG
BspHI 4773 TCATGA T^CATGA
5345 TCATGA
BspMI 67 ACCTGC ACCTGC(4/8)
580 ACCTGC
BsrBI 118 CCGCTC CCGCTC(-3/-3)
3406 CCGCTC
EcoNI 3566 CCTCTCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
7508 CCTTAAGTAGG
EcoRI 919 GAATTC G^AATTC
6941 GAATTC
EcoT22I 4527 ATGCAT ATGCA^T
7274 ATGCAT
FauI 19 CCCGC CCCGC(4/6)
1364 GCGGG
HincII 710 GTTGAC GT[CT]^[GA]AC
5070 GTCAAC
HindII 710 GTTGAC GT[CT]^[GA]AC
5070 GTCAAC
MfeI 1589 CAATTG C^AATTG
5913 CAATTG
MunI 1589 CAATTG C^AATTG
5913 CAATTG
NciI 11 CCGGG CC^[GC]GG
52 CCGGG
NsiI 4527 ATGCAT ATGCA^T
7274 ATGCAT
PmaCI 3392 CACGTG CAC^GTG
4294 CACGTG
PmlI 3392 CACGTG CAC^GTG
4294 CACGTG
PvuII 218 CAGCTG CAG^CTG
7213 CAGCTG
RcaI 4773 TCATGA T^CATGA
5345 TCATGA
SapI 306 GAAGAGC GCTCTTC(1/4)
5569 GAAGAGC
SexAI 3583 ACCAGGT A^CC[AT]GGT
3783 ACCTGGT
XmnI 1047 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3468 GAATCGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflII AflIII AluI AlwI AlwNI
ApoI AspHI AspI AvaII BanI
BanII BbsI BbvI BfaI BfrI
BglII BmyI BpmI BpuAI BsaAI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspLU11I BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraIII EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NdeI NdeII NlaIII NlaIV NspI
PflFI PleI PstI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XhoII