##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccII AclI AcyI AgeI
AhdI Alw44I ApaLI AscI AspEI
AviII AvrII BbeI BlnI BsaBI
BsaHI BsgI BsiEI BsiWI BsmBI
BspDI BsrBI BsrFI BssHII Bst1107I
BstUI BstZ17I Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco52I
FseI FspI Hin1I HindIII KasI
KspI MamI MluI MvnI NaeI
NarI NgoMIV NheI NotI NruI
PacI PinAI PmeI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SpeI Sse8387I StuI SwaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 2767 TCCGGA T^CCGGA
AocI 1454 CCTCAGG CC^T[ACGT]AGG
Aor51HI 11 AGCGCT AGC^GCT
AseI 5485 ATTAAT AT^TAAT
AsnI 5485 ATTAAT AT^TAAT
AspI 1879 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 2345 GCACTGAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 4407 CCCGG CC^[GC]GG
BglI 5233 GCCTGTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2650 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2650 GCTAAGC GC^T[ACGT]AGC
BsaWI 2767 TCCGGA [AT]^CCGG[AT]
BseAI 2767 TCCGGA T^CCGGA
BspEI 2767 TCCGGA T^CCGGA
BspLU11I 6621 ACATGT A^CATGT
BspMI 5549 ACCTGC ACCTGC(4/8)
BssSI 1082 CTCGTG CACGAG(-5/-1)
BstBI 4922 TTCGAA TT^CGAA
BstEII 3639 GGTGACC G^GT[ACGT]ACC
BstPI 3639 GGTGACC G^GT[ACGT]ACC
Bsu36I 1454 CCTCAGG CC^T[ACGT]AGG
CelII 2650 GCTAAGC GC^T[ACGT]AGC
CfoI 12 GCGC GCG^C
DraIII 2615 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 11 AGCGCT AGC^GCT
Eco81I 1454 CCTCAGG CC^T[ACGT]AGG
EcoO65I 3639 GGTGACC G^GT[ACGT]ACC
EcoRV 4422 GATATC GAT^ATC
EcoT22I 2188 ATGCAT ATGCA^T
FauI 44 GCGGG CCCGC(4/6)
HaeII 11 AGCGCT [GA]GCGC^[CT]
HgaI 481 GACGC GACGC(5/10)
HhaI 12 GCGC GCG^C
HinP1I 12 GCGC G^CGC
MfeI 2970 CAATTG C^AATTG
MroI 2767 TCCGGA T^CCGGA
MunI 2970 CAATTG C^AATTG
NciI 4407 CCCGG CC^[GC]GG
NsiI 2188 ATGCAT ATGCA^T
NspV 4922 TTCGAA TT^CGAA
PflFI 1879 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 5485 ATTAAT AT^TAAT
SfuI 4922 TTCGAA TT^CGAA
SnaBI 5185 TACGTA TAC^GTA
SphI 5193 GCATGC GCATG^C
SspI 2407 AATATT AAT^ATT
Tth111I 1879 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 967 CCAGAGGGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 1733 GGTACC G^GTACC
4772 GGTACC
AflII 1069 CTTAAG C^TTAAG
3253 CTTAAG
AflIII 6384 ACGTGT A^C[GA][CT]GT
6621 ACATGT
ApaI 625 GGGCCC GGGCC^C
963 GGGCCC
Asp718I 1733 GGTACC G^GTACC
4772 GGTACC
BalI 1376 TGGCCA TGG^CCA
3051 TGGCCA
BbrPI 2999 CACGTG CAC^GTG
5581 CACGTG
BciVI 2600 GGATAC GTATCC(6/5)
2114 GTATCC
BfrI 1069 CTTAAG C^TTAAG
3253 CTTAAG
BsaI 909 GGTCTC GGTCTC(1/5)
1190 GGTCTC
BseRI 4430 CTCCTC GAGGAG(10/8)
4740 CTCCTC
BspHI 1595 TCATGA T^CATGA
1817 TCATGA
DsaI 5079 CCATGG C^C[GA][CT]GG
6578 CCATGG
HapII 2768 CCGG C^CGG
4408 CCGG
HpaII 2768 CCGG C^CGG
4408 CCGG
KpnI 1733 GGTACC GGTAC^C
4772 GGTACC
MluNI 1376 TGGCCA TGG^CCA
3051 TGGCCA
MscI 1376 TGGCCA TGG^CCA
3051 TGGCCA
MspI 2768 CCGG C^CGG
4408 CCGG
NcoI 5079 CCATGG C^CATGG
6578 CCATGG
NdeI 2138 CATATG CA^TATG
3961 CATATG
PaeR7I 4961 CTCGAG C^TCGAG
6200 CTCGAG
PmaCI 2999 CACGTG CAC^GTG
5581 CACGTG
PmlI 2999 CACGTG CAC^GTG
5581 CACGTG
PstI 1127 CTGCAG CTGCA^G
2255 CTGCAG
RcaI 1595 TCATGA T^CATGA
1817 TCATGA
SacI 843 GAGCTC GAGCT^C
3517 GAGCTC
SapI 4975 GAAGAGC GCTCTTC(1/4)
5400 GAAGAGC
XhoI 4961 CTCGAG C^TCGAG
6200 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BamHI BanI BanII
BbsI BbvCI BbvI BclI BfaI
BglII BmyI BpmI BpuAI BsaAI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BsrDI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoNI EcoO109I EcoRI
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HincII HindII HinfI HpaI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PshAI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XhoII XmnI