##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AccI AflII AgeI AocI AscI
AseI AsnI AviII BclI BfrI
BglII BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CpoI Eco81I
EcoO65I EcoRI EcoRV FbaI FspI
HpaI KspI MfeI MluI MunI
NheI NotI NruI NspV PacI
PinAI PmeI PshAI PshBI PvuI
RsrII SacII SalI ScaI SfuI
SgrAI SnaBI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 3179 GGTACC G^GTACC
AflIII 7019 ACATGT A^C[GA][CT]GT
AhdI 5532 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 3179 GGTACC G^GTACC
AspEI 5532 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1732 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 3160 CCTCAGC CCTCAGC(-5/-2)
BciVI 3642 GTATCC GTATCC(6/5)
BsiEI 3139 CGGCCG CG[GA][CT]^CG
BsiWI 1245 CGTACG C^GTACG
BspDI 2738 ATCGAT AT^CGAT
BspLU11I 7019 ACATGT A^CATGT
BssSI 4900 CACGAG CACGAG(-5/-1)
ClaI 2738 ATCGAT AT^CGAT
DrdI 6530 GACCCTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3139 CGGCCG C^GGCCG
Eam1105I 5532 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3139 CGGCCG C^GGCCG
Eco52I 3139 CGGCCG C^GGCCG
EcoT22I 614 ATGCAT ATGCA^T
FseI 3140 GGCCGGCC GGCCGG^CC
HindIII 437 AAGCTT A^AGCTT
KpnI 3179 GGTACC GGTAC^C
NsiI 614 ATGCAT ATGCA^T
PaeR7I 1 CTCGAG C^TCGAG
PflFI 1732 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SbfI 3502 CCTGCAGG CCTGCA^GG
SmaI 3527 CCCGGG CCC^GGG
SpeI 4678 ACTAGT A^CTAGT
Sse8387I 3502 CCTGCAGG CCTGCA^GG
SspI 5359 AATATT AAT^ATT
Tth111I 1732 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1 CTCGAG C^TCGAG
XmaCI 3527 CCCGGG C^CCGGG
XmaI 3527 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AatII 1013 GACGTC GACGT^C
2690 GACGTC
Alw44I 4295 GTGCAC G^TGCAC
4841 GTGCAC
Aor51HI 2824 AGCGCT AGC^GCT
3164 AGCGCT
ApaLI 4295 GTGCAC G^TGCAC
4841 GTGCAC
Asp700I 4345 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5982 GAATCTGTTC
AvrII 4854 CCTAGG C^CTAGG
6094 CCTAGG
BamHI 1339 GGATCC G^GATCC
2377 GGATCC
BbeI 3283 GGCGCC GGCGC^C
3469 GGCGCC
BbrPI 1616 CACGTG CAC^GTG
1637 CACGTG
BlnI 4854 CCTAGG C^CTAGG
6094 CCTAGG
BsaBI 925 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6126 GATTGCGATC
Bsp1407I 879 TGTACA T^GTACA
1182 TGTACA
BspHI 4560 TCATGA T^CATGA
6807 TCATGA
BsrGI 879 TGTACA T^GTACA
1182 TGTACA
BstAPI 921 GCAGGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
7172 GCACGTACTGC
DraI 32 TTTAAA TTT^AAA
6253 TTTAAA
Eco47III 2824 AGCGCT AGC^GCT
3164 AGCGCT
HincII 4541 GTCAAC GT[CT]^[GA]AC
6527 GTTGAC
HindII 4541 GTCAAC GT[CT]^[GA]AC
6527 GTTGAC
KasI 3283 GGCGCC G^GCGCC
3469 GGCGCC
MamI 925 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6126 GATTGCGATC
NarI 3283 GGCGCC GG^CGCC
3469 GGCGCC
NdeI 508 CATATG CA^TATG
5546 CATATG
PmaCI 1616 CACGTG CAC^GTG
1637 CACGTG
PmlI 1616 CACGTG CAC^GTG
1637 CACGTG
RcaI 4560 TCATGA T^CATGA
6807 TCATGA
SexAI 396 ACCTGGT A^CC[AT]GGT
2265 ACCAGGT
SfiI 3364 GGCCTTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3500 GGCCTGCAGGGCC
SfoI 3283 GGCGCC GGC^GCC
3469 GGCGCC
SspBI 879 TGTACA T^GTACA
1182 TGTACA
XmnI 4345 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5982 GAATCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccII AccIII AciI AclI AcsI
AcyI AfaI AluI AlwI AlwNI
ApaI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcgI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI Psp1406I PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII