##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccI AccII AccIII AgeI
Alw44I Aor51HI ApaLI AscI BamHI
BbeI BsaBI BseAI BsiEI BsiWI
BspEI BsrBI BssHII BssSI Bst1107I
BstBI BstUI BstZ17I CpoI DraIII
DrdI EagI EclXI Eco47III Eco52I
FseI HindIII KasI KspI MamI
MluI MroI MvnI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I StuI
XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 6512 GGTACC G^GTACC
AclI 1236 AACGTT AA^CGTT
AcyI 2221 GGCGTC G[GA]^CG[CT]C
AflII 4299 CTTAAG C^TTAAG
AocI 4455 CCTTAGG CC^T[ACGT]AGG
ApaI 5803 GGGCCC GGGCC^C
Asp718I 6512 GGTACC G^GTACC
AspI 4261 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3261 TGCGCA TGC^GCA
BbvCI 2071 GCTGAGG CCTCAGC(-5/-2)
BcgI 1853 CGACGGCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4299 CTTAAG C^TTAAG
BglII 6012 AGATCT A^GATCT
BlpI 3060 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3060 GCTCAGC GC^T[ACGT]AGC
BsaHI 2221 GGCGTC G[GA]^CG[CT]C
BsaI 2875 GAGACC GGTCTC(1/5)
BsaWI 3650 ACCGGA [AT]^CCGG[AT]
BspDI 2513 ATCGAT AT^CGAT
BsrFI 3342 ACCGGC [GA]^CCGG[CT]
BstEII 4879 GGTAACC G^GT[ACGT]ACC
BstPI 4879 GGTAACC G^GT[ACGT]ACC
Bsu36I 4455 CCTTAGG CC^T[ACGT]AGG
CelII 3060 GCTCAGC GC^T[ACGT]AGC
Cfr10I 3342 ACCGGC [GA]^CCGG[CT]
ClaI 2513 ATCGAT AT^CGAT
Eco81I 4455 CCTTAGG CC^T[ACGT]AGG
EcoO65I 4879 GGTAACC G^GT[ACGT]ACC
FspI 3261 TGCGCA TGC^GCA
HaeII 4999 GGCGCT [GA]GCGC^[CT]
Hin1I 2221 GGCGTC G[GA]^CG[CT]C
HpaI 5929 GTTAAC GTT^AAC
KpnI 6512 GGTACC GGTAC^C
MfeI 4772 CAATTG C^AATTG
MunI 4772 CAATTG C^AATTG
NdeI 6608 CATATG CA^TATG
PflFI 4261 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 739 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1236 AACGTT AA^CGTT
SacI 2017 GAGCTC GAGCT^C
SmaI 6230 CCCGGG CCC^GGG
SphI 5444 GCATGC GCATG^C
SwaI 4356 ATTTAAAT ATTT^AAAT
Tth111I 4261 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 6230 CCCGGG C^CCGGG
XmaI 6230 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AhdI 1622 GACGAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2079 GACACTGTGTC
Asp700I 3677 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5679 GAATGTTTTC
AspEI 1622 GACGAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2079 GACACTGTGTC
AvrII 1229 CCTAGG C^CTAGG
2834 CCTAGG
BanI 2813 GGTGCC G^G[CT][GA]CC
6512 GGTACC
BbrPI 4828 CACGTG CAC^GTG
5364 CACGTG
BciVI 5167 GGATAC GTATCC(6/5)
6670 GTATCC
BclI 504 TGATCA T^GATCA
960 TGATCA
BglI 1482 GCCTCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
6446 GCCACCATGGC
BlnI 1229 CCTAGG C^CTAGG
2834 CCTAGG
BpmI 2433 CTGGAG CTGGAG(16/14)
3075 CTGGAG
BsmBI 2223 CGTCTC CGTCTC(1/5)
3311 GAGACG
Bsp1407I 5424 TGTACA T^GTACA
5434 TGTACA
BspLU11I 2648 ACATGT A^CATGT
5550 ACATGT
BsrGI 5424 TGTACA T^GTACA
5434 TGTACA
Eam1105I 1622 GACGAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2079 GACACTGTGTC
EcoNI 2273 CCTTCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2396 CCTTTCCGAGG
EcoRV 1437 GATATC GAT^ATC
2546 GATATC
FbaI 504 TGATCA T^GATCA
960 TGATCA
HgaI 3313 GACGC GACGC(5/10)
2222 GCGTC
PflMI 1386 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3422 CCAAAATGTGG
PmaCI 4828 CACGTG CAC^GTG
5364 CACGTG
PmlI 4828 CACGTG CAC^GTG
5364 CACGTG
SspBI 5424 TGTACA T^GTACA
5434 TGTACA
Van91I 1386 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3422 CCAAAATGTGG
XmnI 3677 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5679 GAATGTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
AspHI AvaI AvaII BalI BanII
BbsI BbvI BcnI BfaI BmyI
BpuAI BsaAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspHI BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRI
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NsiI NspI PleI
PpuMI PshBI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XcmI XhoII