##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AhdI Alw44I ApaI ApaLI AscI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BanI BbeI BbrPI
BcgI BglI BlnI BsaHI BsaI
BseAI BspDI BspEI BsrBI BsrFI
BssHII BssSI Cfr10I ClaI CpoI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco52I EcoRI FauI
FseI FspI HgaI Hin1I KasI
KpnI KspI MluI MluNI MroI
MscI NaeI NarI NcoI NgoMIV
NheI NotI PacI PflFI PinAI
PmaCI PmeI PmlI PvuI RsrII
SacII SalI SapI SbfI SfiI
SfoI SgrAI Sse8387I Tth111I XcmI
XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccII 6913 CGCG CG^CG
AocI 242 CCTCAGG CC^T[ACGT]AGG
Aor51HI 5736 AGCGCT AGC^GCT
BlpI 3005 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3005 GCTAAGC GC^T[ACGT]AGC
BsaBI 1283 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 6291 TCCGGT [AT]^CCGG[AT]
BsiEI 1215 CGGTCG CG[GA][CT]^CG
BsiWI 940 CGTACG C^GTACG
BstAPI 4843 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 5848 TTCGAA TT^CGAA
BstEII 1424 GGTTACC G^GT[ACGT]ACC
BstPI 1424 GGTTACC G^GT[ACGT]ACC
BstUI 6913 CGCG CG^CG
BstXI 5038 CCAGAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 242 CCTCAGG CC^T[ACGT]AGG
CelII 3005 GCTAAGC GC^T[ACGT]AGC
Eco47III 5736 AGCGCT AGC^GCT
Eco81I 242 CCTCAGG CC^T[ACGT]AGG
EcoNI 3636 CCTTGGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1424 GGTTACC G^GT[ACGT]ACC
EcoRV 4315 GATATC GAT^ATC
HindIII 864 AAGCTT A^AGCTT
MamI 1283 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 972 CAGCTG C[AC]G^C[GT]G
MvnI 6913 CGCG CG^CG
NdeI 2584 CATATG CA^TATG
NruI 6912 TCGCGA TCG^CGA
NspV 5848 TTCGAA TT^CGAA
PflMI 1183 CCAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1969 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 6544 GACGGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 972 CAGCTG CAG^CTG
SacI 3101 GAGCTC GAGCT^C
SexAI 322 ACCAGGT A^CC[AT]GGT
SfuI 5848 TTCGAA TT^CGAA
SmaI 4856 CCCGGG CCC^GGG
StuI 3387 AGGCCT AGG^CCT
SwaI 1322 ATTTAAAT ATTT^AAAT
Van91I 1183 CCAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 4856 CCCGGG C^CCGGG
XmaI 4856 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 3590 CCGC CCGC(-3/-1)
1214 GCGG
AseI 49 ATTAAT AT^TAAT
4701 ATTAAT
AsnI 49 ATTAAT AT^TAAT
4701 ATTAAT
BamHI 2104 GGATCC G^GATCC
3349 GGATCC
BsmBI 4950 GAGACG CGTCTC(1/5)
6542 GAGACG
BspHI 5326 TCATGA T^CATGA
6136 TCATGA
CfoI 5737 GCGC GCG^C
6031 GCGC
EcoT22I 3224 ATGCAT ATGCA^T
6907 ATGCAT
HaeII 5736 AGCGCT [GA]GCGC^[CT]
6030 GGCGCT
HhaI 5737 GCGC GCG^C
6031 GCGC
HinP1I 5737 GCGC G^CGC
6031 GCGC
HpaI 5224 GTTAAC GTT^AAC
5659 GTTAAC
MfeI 4079 CAATTG C^AATTG
4554 CAATTG
MunI 4079 CAATTG C^AATTG
4554 CAATTG
NsiI 3224 ATGCAT ATGCA^T
6907 ATGCAT
PaeR7I 2997 CTCGAG C^TCGAG
6592 CTCGAG
PshBI 49 ATTAAT AT^TAAT
4701 ATTAAT
RcaI 5326 TCATGA T^CATGA
6136 TCATGA
SnaBI 2131 TACGTA TAC^GTA
5192 TACGTA
SphI 3222 GCATGC GCATG^C
4911 GCATGC
XhoI 2997 CTCGAG C^TCGAG
6592 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AclI AcsI AfaI AflII
AflIII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BanII BbsI
BbvCI BbvI BciVI BclI BcnI
BfaI BfrI BglII BmyI BpmI
BpuAI BsaAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspLU11I
BspMI BsrDI BsrGI BsrI BssKI
Bst1107I BstF5I BstNI BstYI BstZ17I
Cac8I Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRII EcoT14I
FbaI Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
Psp1406I PstI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SpeI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XhoII