##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
Acc65I AccII AccIII AgeI Aor51HI
AscI Asp718I AvrII BbeI BbrPI
BbvCI BciVI BlnI BseAI BsiEI
BsiWI BsmBI BspEI BsrBI BsrFI
BssHII BssSI BstUI Cfr10I CpoI
EagI EclXI Eco47III Eco52I EcoNI
FseI HaeII KasI KpnI KspI
MluI MroI MvnI NaeI NarI
NgoMIV NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PvuI RsrII SacI SacII SalI
SbfI SfiI SfoI SgrAI SnaBI
SphI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 2531 GACGTC GACGT^C
AclI 5252 AACGTT AA^CGTT
AcyI 2531 GACGTC G[GA]^CG[CT]C
AhdI 6914 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 6 CCTTAGG CC^T[ACGT]AGG
ApaI 7937 GGGCCC GGGCC^C
AspEI 6914 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2620 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2002 TGCGCA TGC^GCA
BcgI 2224 GCATCATTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 550 AGATCT A^GATCT
BlpI 2126 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2126 GCTGAGC GC^T[ACGT]AGC
BsaAI 6811 CACGTA [CT]AC^GT[GA]
BsaHI 2531 GACGTC G[GA]^CG[CT]C
BspDI 2045 ATCGAT AT^CGAT
BstAPI 1513 GCATCACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 6 CCTTAGG CC^T[ACGT]AGG
CelII 2126 GCTGAGC GC^T[ACGT]AGC
ClaI 2045 ATCGAT AT^CGAT
DrdI 6614 GACCACTAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 6914 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 6 CCTTAGG CC^T[ACGT]AGG
EcoRV 7061 GATATC GAT^ATC
EcoT22I 2901 ATGCAT ATGCA^T
FspI 2002 TGCGCA TGC^GCA
HgaI 6253 GCGTC GACGC(5/10)
Hin1I 2531 GACGTC G[GA]^CG[CT]C
HpaI 3822 GTTAAC GTT^AAC
NdeI 6317 CATATG CA^TATG
NheI 2866 GCTAGC G^CTAGC
NsiI 2901 ATGCAT ATGCA^T
PflFI 2620 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 3141 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 5252 AACGTT AA^CGTT
SexAI 2716 ACCAGGT A^CC[AT]GGT
SmaI 7940 CCCGGG CCC^GGG
SpeI 7422 ACTAGT A^CTAGT
StuI 4320 AGGCCT AGG^CCT
Tth111I 2620 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5633 TCTAGA T^CTAGA
XmaCI 7940 CCCGGG C^CCGGG
XmaI 7940 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 4569 GTATAC GT^[AC][GT]AC
8119 GTATAC
Alw44I 738 GTGCAC G^TGCAC
2636 GTGCAC
AlwNI 247 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
897 CAGCTCCTG
ApaLI 738 GTGCAC G^TGCAC
2636 GTGCAC
AvaI 7908 CTCGGG C^[CT]CG[GA]G
7940 CCCGGG
BamHI 2154 GGATCC G^GATCC
7011 GGATCC
BanI 1363 GGCACC G^G[CT][GA]CC
2519 GGTGCC
BclI 2752 TGATCA T^GATCA
4430 TGATCA
BglI 700 GCCACAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
7837 GCCAACATGGC
BsaBI 2750 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4116 GATTAAAATC
BsoBI 7908 CTCGGG C^[CT]CG[GA]G
7940 CCCGGG
Bsp1407I 2508 TGTACA T^GTACA
3835 TGTACA
BsrGI 2508 TGTACA T^GTACA
3835 TGTACA
Bst1107I 4569 GTATAC GTA^TAC
8119 GTATAC
BstBI 1887 TTCGAA TT^CGAA
5652 TTCGAA
BstZ17I 4569 GTATAC GTA^TAC
8119 GTATAC
CfoI 2003 GCGC GCG^C
2592 GCGC
FauI 5049 CCCGC CCCGC(4/6)
4551 GCGGG
FbaI 2752 TGATCA T^GATCA
4430 TGATCA
HhaI 2003 GCGC GCG^C
2592 GCGC
HinP1I 2003 GCGC G^CGC
2592 GCGC
MamI 2750 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4116 GATTAAAATC
NspV 1887 TTCGAA TT^CGAA
5652 TTCGAA
PflMI 2805 CCATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
7648 CCAGCCCTTGG
PvuII 372 CAGCTG CAG^CTG
1061 CAGCTG
SapI 2849 GCTCTTC GCTCTTC(1/4)
4093 GCTCTTC
ScaI 3623 AGTACT AGT^ACT
5346 AGTACT
SfuI 1887 TTCGAA TT^CGAA
5652 TTCGAA
SspBI 2508 TGTACA T^GTACA
3835 TGTACA
Van91I 2805 CCATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
7648 CCAGCCCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI ApoI AseI AsnI
Asp700I AspHI AvaII BalI BanII
BbsI BbvI BcnI BfaI BfrI
BmyI BpmI BpuAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspHI BspLU11I BspMI BsrDI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI EcoO109I EcoO65I EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MunI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PshBI PstI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI