##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI Aor51HI AscI Asp718I AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BcgI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BseAI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraIII EagI EclXI Eco47III Eco52I
EcoO65I FauI FbaI FseI FspI
HaeII HhaI HinP1I KasI KpnI
KspI MflI MluI MroI MvnI
NaeI NarI NciI NgoMIV NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SalI SapI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI SwaI Tth111I XhoI
XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 2009 GACGTC GACGT^C
AcyI 2009 GACGTC G[GA]^CG[CT]C
Alw44I 5244 GTGCAC G^TGCAC
AocI 4035 CCTGAGG CC^T[ACGT]AGG
ApaI 4798 GGGCCC GGGCC^C
ApaLI 5244 GTGCAC G^TGCAC
BalI 4745 TGGCCA TGG^CCA
BanI 5192 GGTGCC G^G[CT][GA]CC
BbsI 2033 GAAGAC GAAGAC(2/6)
BbvCI 5503 GCTGAGG CCTCAGC(-5/-2)
BciVI 3809 GTATCC GTATCC(6/5)
BpuAI 2033 GAAGAC GAAGAC(2/6)
BsaAI 3435 CACGTA [CT]AC^GT[GA]
BsaBI 1603 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2009 GACGTC G[GA]^CG[CT]C
BsaWI 1011 ACCGGA [AT]^CCGG[AT]
Bsu36I 4035 CCTGAGG CC^T[ACGT]AGG
DrdI 1933 GACATGAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 4745 TGGCCA [CT]^GGCC[GA]
EarI 4600 GAAGAG CTCTTC(1/4)
Eco81I 4035 CCTGAGG CC^T[ACGT]AGG
EcoRV 6281 GATATC GAT^ATC
HapII 1012 CCGG C^CGG
HgaI 1480 GACGC GACGC(5/10)
Hin1I 2009 GACGTC G[GA]^CG[CT]C
HpaII 1012 CCGG C^CGG
Ksp632I 4600 GAAGAG CTCTTC(1/4)
MamI 1603 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 443 CAATTG C^AATTG
MluNI 4745 TGGCCA TGG^CCA
MscI 4745 TGGCCA TGG^CCA
MspI 1012 CCGG C^CGG
MunI 443 CAATTG C^AATTG
NcoI 2028 CCATGG C^CATGG
NheI 1693 GCTAGC G^CTAGC
PflMI 3341 CCAAATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 4905 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 4557 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 5138 CCTGCAGG CCTGCA^GG
SexAI 1515 ACCAGGT A^CC[AT]GGT
SmlI 2637 CTCAAG C^T[CT][GA]AG
SpeI 219 ACTAGT A^CTAGT
Sse8387I 5138 CCTGCAGG CCTGCA^GG
StuI 4818 AGGCCT AGG^CCT
Van91I 3341 CCAAATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2646 CCAGGTAGTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 4400 GTCTAC GT^[AC][GT]AC
4554 GTAGAC
AciI 5744 CCGC CCGC(-3/-1)
5105 GCGG
AhdI 215 GACAACTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4907 GACCCTGAGTC
AlwI 3108 GATCC GGATC(4/5)
4029 GGATC
AspEI 215 GACAACTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4907 GACCCTGAGTC
AvaII 4906 GGACC G^G[AT]CC
5283 GGACC
BsaI 5134 GAGACC GGTCTC(1/5)
4784 GGTCTC
BseRI 5226 CTCCTC GAGGAG(10/8)
5181 GAGGAG
BsgI 4216 CTGCAC GTGCAG(16/14)
5158 GTGCAG
BspHI 1850 TCATGA T^CATGA
2005 TCATGA
BstXI 4019 CCAGGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4138 CCAGCTACTTGG
DraII 4797 AGGGCCC [GA]G^G[ACGT]CC[CT]
4905 GGGACCC
DsaI 2028 CCATGG C^C[GA][CT]GG
5188 CCGTGG
Eam1105I 215 GACAACTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4907 GACCCTGAGTC
EcoO109I 4797 AGGGCCC [GA]G^G[ACGT]CC[CT]
4905 GGGACCC
EcoRI 1423 GAATTC G^AATTC
3220 GAATTC
EcoT22I 1089 ATGCAT ATGCA^T
4516 ATGCAT
HpaI 2573 GTTAAC GTT^AAC
2618 GTTAAC
MspA1I 28 CAGCTG C[AC]G^C[GT]G
295 CAGCTG
NsiI 1089 ATGCAT ATGCA^T
4516 ATGCAT
PstI 4221 CTGCAG CTGCA^G
5139 CTGCAG
PvuII 28 CAGCTG CAG^CTG
295 CAGCTG
RcaI 1850 TCATGA T^CATGA
2005 TCATGA
XbaI 428 TCTAGA T^CTAGA
5549 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwNI
ApoI AseI AsnI Asp700I AspHI
BanII BbvI BfaI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspLU11I
BspMI BsrGI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraI EcoNI EcoRII EcoT14I Fnu4HI
FokI HaeIII HincII HindII HindIII
HinfI HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MvaI MwoI NdeI NdeII
NlaIII NlaIV NspI PleI PshBI
RsaI SacI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XmnI