################################## # NO CUT ENZYMES ( 54 enzymes) # ################################## AatII Acc65I AccIII AgeI Alw44I Aor51HI ApaLI AscI Asp718I BciVI BsaBI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspLU11I BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoRV FseI HpaI KpnI MamI MluI MroI NaeI NgoMIV NotI NruI NspV PacI PinAI PmeI PvuI RsrII SalI SbfI SfuI SgrAI SphI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AclI 182 AACGTT AA^CGTT AflII 1250 CTTAAG C^TTAAG AhdI 6480 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 825 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 6480 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1528 TGCGCA TGC^GCA AvrII 2336 CCTAGG C^CTAGG BamHI 4519 GGATCC G^GATCC BbeI 2285 GGCGCC GGCGC^C BbrPI 5646 CACGTG CAC^GTG BcgI 6482 CGAGGTGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 1250 CTTAAG C^TTAAG BglII 3265 AGATCT A^GATCT BlnI 2336 CCTAGG C^CTAGG BsaI 1706 GAGACC GGTCTC(1/5) BsmBI 6400 CGTCTC CGTCTC(1/5) Bsp1407I 6370 TGTACA T^GTACA BsrGI 6370 TGTACA T^GTACA Eam1105I 6480 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 3150 GAATTC G^AATTC EcoT22I 4680 ATGCAT ATGCA^T FspI 1528 TGCGCA TGC^GCA KasI 2285 GGCGCC G^GCGCC KspI 5745 CCGCGG CCGC^GG MfeI 4726 CAATTG C^AATTG MunI 4726 CAATTG C^AATTG NarI 2285 GGCGCC GG^CGCC NheI 6473 GCTAGC G^CTAGC NsiI 4680 ATGCAT ATGCA^T PmaCI 5646 CACGTG CAC^GTG PmlI 5646 CACGTG CAC^GTG Psp1406I 182 AACGTT AA^CGTT SacII 5745 CCGCGG CCGC^GG SapI 3199 GAAGAGC GCTCTTC(1/4) ScaI 272 AGTACT AGT^ACT SfiI 5766 GGCCAGAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 2285 GGCGCC GGC^GCC SmaI 5944 CCCGGG CCC^GGG SnaBI 2402 TACGTA TAC^GTA SspBI 6370 TGTACA T^GTACA SspI 6753 AATATT AAT^ATT XmaCI 5944 CCCGGG C^CCGGG XmaI 5944 CCCGGG C^CCGGG XmnI 825 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcyI 2285 GGCGCC G[GA]^CG[CT]C 4282 GGCGTC AflIII 5035 ACGTGT A^C[GA][CT]GT 6397 ACACGT AseI 362 ATTAAT AT^TAAT 4810 ATTAAT AsnI 362 ATTAAT AT^TAAT 4810 ATTAAT BclI 2754 TGATCA T^GATCA 6516 TGATCA BsaAI 2402 TACGTA [CT]AC^GT[GA] 5646 CACGTG BsaHI 2285 GGCGCC G[GA]^CG[CT]C 4282 GGCGTC BspHI 466 TCATGA T^CATGA 1392 TCATGA BsrFI 5149 GCCGGT [GA]^CCGG[CT] 6432 ACCGGC BstAPI 214 GCAGTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5220 GCAGCCACTGC BstEII 2061 GGTCACC G^GT[ACGT]ACC 6143 GGTGACC BstPI 2061 GGTCACC G^GT[ACGT]ACC 6143 GGTGACC Cfr10I 5149 GCCGGT [GA]^CCGG[CT] 6432 ACCGGC EcoO65I 2061 GGTCACC G^GT[ACGT]ACC 6143 GGTGACC FbaI 2754 TGATCA T^GATCA 6516 TGATCA HaeII 2285 GGCGCC [GA]GCGC^[CT] 3771 GGCGCT Hin1I 2285 GGCGCC G[GA]^CG[CT]C 4282 GGCGTC HindIII 2974 AAGCTT A^AGCTT 6219 AAGCTT NdeI 1484 CATATG CA^TATG 5013 CATATG PaeR7I 3010 CTCGAG C^TCGAG 4457 CTCGAG PshAI 3496 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 5321 GACCCTGGTC PshBI 362 ATTAAT AT^TAAT 4810 ATTAAT RcaI 466 TCATGA T^CATGA 1392 TCATGA SexAI 3507 ACCAGGT A^CC[AT]GGT 3535 ACCAGGT SpeI 206 ACTAGT A^CTAGT 5087 ACTAGT XcmI 237 CCATCCTCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 6242 CCAGTCGAAGATTGG XhoI 3010 CTCGAG C^TCGAG 4457 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 133 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwI AlwNI AocI ApaI ApoI AspHI AspI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XhoII