##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII Acc65I AccIII AgeI Alw44I
Aor51HI ApaLI AscI Asp718I BciVI
BsaBI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BspLU11I BssHII BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoRV FseI HpaI KpnI MamI
MluI MroI NaeI NgoMIV NotI
NruI NspV PacI PinAI PmeI
PvuI RsrII SalI SbfI SfuI
SgrAI SphI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AclI 182 AACGTT AA^CGTT
AflII 1250 CTTAAG C^TTAAG
AhdI 6480 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 825 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 6480 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1528 TGCGCA TGC^GCA
AvrII 2336 CCTAGG C^CTAGG
BamHI 4519 GGATCC G^GATCC
BbeI 2285 GGCGCC GGCGC^C
BbrPI 5646 CACGTG CAC^GTG
BcgI 6482 CGAGGTGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1250 CTTAAG C^TTAAG
BglII 3265 AGATCT A^GATCT
BlnI 2336 CCTAGG C^CTAGG
BsaI 1706 GAGACC GGTCTC(1/5)
BsmBI 6400 CGTCTC CGTCTC(1/5)
Bsp1407I 6370 TGTACA T^GTACA
BsrGI 6370 TGTACA T^GTACA
Eam1105I 6480 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3150 GAATTC G^AATTC
EcoT22I 4680 ATGCAT ATGCA^T
FspI 1528 TGCGCA TGC^GCA
KasI 2285 GGCGCC G^GCGCC
KspI 5745 CCGCGG CCGC^GG
MfeI 4726 CAATTG C^AATTG
MunI 4726 CAATTG C^AATTG
NarI 2285 GGCGCC GG^CGCC
NheI 6473 GCTAGC G^CTAGC
NsiI 4680 ATGCAT ATGCA^T
PmaCI 5646 CACGTG CAC^GTG
PmlI 5646 CACGTG CAC^GTG
Psp1406I 182 AACGTT AA^CGTT
SacII 5745 CCGCGG CCGC^GG
SapI 3199 GAAGAGC GCTCTTC(1/4)
ScaI 272 AGTACT AGT^ACT
SfiI 5766 GGCCAGAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2285 GGCGCC GGC^GCC
SmaI 5944 CCCGGG CCC^GGG
SnaBI 2402 TACGTA TAC^GTA
SspBI 6370 TGTACA T^GTACA
SspI 6753 AATATT AAT^ATT
XmaCI 5944 CCCGGG C^CCGGG
XmaI 5944 CCCGGG C^CCGGG
XmnI 825 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 2285 GGCGCC G[GA]^CG[CT]C
4282 GGCGTC
AflIII 5035 ACGTGT A^C[GA][CT]GT
6397 ACACGT
AseI 362 ATTAAT AT^TAAT
4810 ATTAAT
AsnI 362 ATTAAT AT^TAAT
4810 ATTAAT
BclI 2754 TGATCA T^GATCA
6516 TGATCA
BsaAI 2402 TACGTA [CT]AC^GT[GA]
5646 CACGTG
BsaHI 2285 GGCGCC G[GA]^CG[CT]C
4282 GGCGTC
BspHI 466 TCATGA T^CATGA
1392 TCATGA
BsrFI 5149 GCCGGT [GA]^CCGG[CT]
6432 ACCGGC
BstAPI 214 GCAGTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5220 GCAGCCACTGC
BstEII 2061 GGTCACC G^GT[ACGT]ACC
6143 GGTGACC
BstPI 2061 GGTCACC G^GT[ACGT]ACC
6143 GGTGACC
Cfr10I 5149 GCCGGT [GA]^CCGG[CT]
6432 ACCGGC
EcoO65I 2061 GGTCACC G^GT[ACGT]ACC
6143 GGTGACC
FbaI 2754 TGATCA T^GATCA
6516 TGATCA
HaeII 2285 GGCGCC [GA]GCGC^[CT]
3771 GGCGCT
Hin1I 2285 GGCGCC G[GA]^CG[CT]C
4282 GGCGTC
HindIII 2974 AAGCTT A^AGCTT
6219 AAGCTT
NdeI 1484 CATATG CA^TATG
5013 CATATG
PaeR7I 3010 CTCGAG C^TCGAG
4457 CTCGAG
PshAI 3496 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5321 GACCCTGGTC
PshBI 362 ATTAAT AT^TAAT
4810 ATTAAT
RcaI 466 TCATGA T^CATGA
1392 TCATGA
SexAI 3507 ACCAGGT A^CC[AT]GGT
3535 ACCAGGT
SpeI 206 ACTAGT A^CTAGT
5087 ACTAGT
XcmI 237 CCATCCTCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
6242 CCAGTCGAAGATTGG
XhoI 3010 CTCGAG C^TCGAG
4457 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AspI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XbaI XhoII