##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII Acc65I AflII AgeI AhdI
AscI AseI AsnI Asp718I AspEI
AvrII BfrI BglII BlnI BsiWI
BspHI BspLU11I BssHII Bst1107I BstBI
BstZ17I CpoI DrdI Eam1105I EcoRI
EcoRV EcoT22I FseI HpaI KpnI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshBI RcaI
RsrII SalI SbfI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccI 6116 GTAGAC GT^[AC][GT]AC
AccIII 110 TCCGGA T^CCGGA
AclI 3684 AACGTT AA^CGTT
AcsI 4126 AAATTC [GA]^AATT[CT]
AflIII 5684 ACACGT A^C[GA][CT]GT
AlwNI 3355 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 6017 CCTGAGG CC^T[ACGT]AGG
ApoI 4126 AAATTC [GA]^AATT[CT]
BcgI 5518 GCAGACGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2581 GGATAC GTATCC(6/5)
BsaBI 2779 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 110 TCCGGA T^CCGGA
BsmBI 1674 CGTCTC CGTCTC(1/5)
BspDI 5308 ATCGAT AT^CGAT
BspEI 110 TCCGGA T^CCGGA
BstAPI 3248 GCATCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1578 GGTCACC G^GT[ACGT]ACC
BstPI 1578 GGTCACC G^GT[ACGT]ACC
Bsu36I 6017 CCTGAGG CC^T[ACGT]AGG
ClaI 5308 ATCGAT AT^CGAT
DraI 6306 TTTAAA TTT^AAA
DraIII 1432 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 6017 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1578 GGTCACC G^GT[ACGT]ACC
HindIII 461 AAGCTT A^AGCTT
MamI 2779 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 110 TCCGGA T^CCGGA
PshAI 4396 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3684 AACGTT AA^CGTT
PvuI 1568 CGATCG CGAT^CG
ScaI 5021 AGTACT AGT^ACT
StuI 1967 AGGCCT AGG^CCT
XbaI 591 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Alw44I 4991 GTGCAC G^TGCAC
6036 GTGCAC
Aor51HI 237 AGCGCT AGC^GCT
2705 AGCGCT
ApaLI 4991 GTGCAC G^TGCAC
6036 GTGCAC
Asp700I 583 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5775 GAACACCTTC
AviII 3232 TGCGCA TGC^GCA
4433 TGCGCA
BamHI 3848 GGATCC G^GATCC
4706 GGATCC
BsaWI 110 TCCGGA [AT]^CCGG[AT]
5732 ACCGGA
BsrFI 38 GCCGGC [GA]^CCGG[CT]
147 GCCGGC
Cfr10I 38 GCCGGC [GA]^CCGG[CT]
147 GCCGGC
EagI 54 CGGCCG C^GGCCG
4863 CGGCCG
EclXI 54 CGGCCG C^GGCCG
4863 CGGCCG
Eco47III 237 AGCGCT AGC^GCT
2705 AGCGCT
Eco52I 54 CGGCCG C^GGCCG
4863 CGGCCG
FspI 3232 TGCGCA TGC^GCA
4433 TGCGCA
NaeI 38 GCCGGC GCC^GGC
147 GCCGGC
NcoI 281 CCATGG C^CATGG
3399 CCATGG
NgoMIV 38 GCCGGC G^CCGGC
147 GCCGGC
NspI 4847 ACATGC [GA]CATG^[CT]
5099 ACATGC
SmlI 287 CTCAAG C^T[CT][GA]AG
2737 CTCAAG
SspI 1742 AATATT AAT^ATT
5815 AATATT
XmnI 583 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5775 GAACACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AspI AvaI
AvaII BalI BanI BanII BbeI
BbrPI BbsI BbvCI BbvI BclI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspMI
BsrBI BsrDI BsrGI BsrI BssKI
BssSI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV PflFI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacI SacII SapI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SfoI
SmaI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII
XmaCI XmaI