################################## # NO CUT ENZYMES ( 56 enzymes) # ################################## AccI AclI Aor51HI AscI AseI AsnI Asp700I AspI AviII BsaBI BsiWI BsmI Bsp1407I BspDI BsrBI BsrGI Bst1107I BstZ17I ClaI CpoI DraI Eco47III EcoRI EcoT22I FseI FspI HindIII HpaI MamI MfeI MluI MunI NdeI NheI NotI NsiI PacI PaeR7I PflFI PmeI PshBI Psp1406I PvuI RsrII SalI ScaI SgrAI SnaBI SpeI SspBI SspI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### Acc65I 366 GGTACC G^GTACC AcsI 2319 AAATTC [GA]^AATT[CT] AflII 1434 CTTAAG C^TTAAG AflIII 1843 ACATGT A^C[GA][CT]GT AgeI 5663 ACCGGT A^CCGGT AhdI 419 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 2319 AAATTC [GA]^AATT[CT] Asp718I 366 GGTACC G^GTACC AspEI 419 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 3916 CACGTG CAC^GTG BbvCI 217 GCTGAGG CCTCAGC(-5/-2) BciVI 3788 GTATCC GTATCC(6/5) BfrI 1434 CTTAAG C^TTAAG BglII 902 AGATCT A^GATCT BsmBI 887 GAGACG CGTCTC(1/5) BspLU11I 1843 ACATGT A^CATGT BstBI 1580 TTCGAA TT^CGAA DrdI 4405 GACCCCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 419 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 5267 GATATC GAT^ATC KpnI 366 GGTACC GGTAC^C NruI 2970 TCGCGA TCG^CGA NspV 1580 TTCGAA TT^CGAA PinAI 5663 ACCGGT A^CCGGT PmaCI 3916 CACGTG CAC^GTG PmlI 3916 CACGTG CAC^GTG SapI 5071 GAAGAGC GCTCTTC(1/4) SbfI 5761 CCTGCAGG CCTGCA^GG SfuI 1580 TTCGAA TT^CGAA SphI 3770 GCATGC GCATG^C Sse8387I 5761 CCTGCAGG CCTGCA^GG TfiI 3701 GAATC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccIII 2230 TCCGGA T^CCGGA 3842 TCCGGA Alw44I 3523 GTGCAC G^TGCAC 4366 GTGCAC ApaLI 3523 GTGCAC G^TGCAC 4366 GTGCAC AvrII 3109 CCTAGG C^CTAGG 3814 CCTAGG BclI 453 TGATCA T^GATCA 1907 TGATCA BlnI 3109 CCTAGG C^CTAGG 3814 CCTAGG BlpI 841 GCTCAGC GC^T[ACGT]AGC 4041 GCTCAGC Bpu1102I 841 GCTCAGC GC^T[ACGT]AGC 4041 GCTCAGC BseAI 2230 TCCGGA T^CCGGA 3842 TCCGGA BspEI 2230 TCCGGA T^CCGGA 3842 TCCGGA BspHI 153 TCATGA T^CATGA 510 TCATGA BsrDI 3985 CATTGC GCAATG(2/0) 5792 GCAATG BssHII 3085 GCGCGC G^CGCGC 4154 GCGCGC BstAPI 547 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4738 GCACCTGCTGC BstXI 1444 CCAGGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1536 CCACAAAGCTGG CelII 841 GCTCAGC GC^T[ACGT]AGC 4041 GCTCAGC EarI 3505 GAAGAG CTCTTC(1/4) 5071 GAAGAG FbaI 453 TGATCA T^GATCA 1907 TGATCA HincII 794 GTTGAC GT[CT]^[GA]AC 4519 GTCAAC HindII 794 GTTGAC GT[CT]^[GA]AC 4519 GTCAAC Ksp632I 3505 GAAGAG CTCTTC(1/4) 5071 GAAGAG MroI 2230 TCCGGA T^CCGGA 3842 TCCGGA MseI 1435 TTAA T^TAA 2555 TTAA PshAI 3004 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3805 GACCCAGGTC RcaI 153 TCATGA T^CATGA 510 TCATGA SfiI 2716 GGCCGGGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 5759 GGCCTGCAGGGCC Tru9I 1435 TTAA T^TAA 2555 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 143 enzymes) # ######################################### AatII AccII AciI AcyI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BalI BamHI BanI BanII BbeI BbsI BbvI BcgI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BssSI BstEII BstF5I BstNI BstPI BstUI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EagI EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SacII Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SfoI SmaI SmlI StuI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI