##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AccI AclI Aor51HI AscI AseI
AsnI Asp700I AspI AviII BsaBI
BsiWI BsmI Bsp1407I BspDI BsrBI
BsrGI Bst1107I BstZ17I ClaI CpoI
DraI Eco47III EcoRI EcoT22I FseI
FspI HindIII HpaI MamI MfeI
MluI MunI NdeI NheI NotI
NsiI PacI PaeR7I PflFI PmeI
PshBI Psp1406I PvuI RsrII SalI
ScaI SgrAI SnaBI SpeI SspBI
SspI SwaI Tth111I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 366 GGTACC G^GTACC
AcsI 2319 AAATTC [GA]^AATT[CT]
AflII 1434 CTTAAG C^TTAAG
AflIII 1843 ACATGT A^C[GA][CT]GT
AgeI 5663 ACCGGT A^CCGGT
AhdI 419 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 2319 AAATTC [GA]^AATT[CT]
Asp718I 366 GGTACC G^GTACC
AspEI 419 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 3916 CACGTG CAC^GTG
BbvCI 217 GCTGAGG CCTCAGC(-5/-2)
BciVI 3788 GTATCC GTATCC(6/5)
BfrI 1434 CTTAAG C^TTAAG
BglII 902 AGATCT A^GATCT
BsmBI 887 GAGACG CGTCTC(1/5)
BspLU11I 1843 ACATGT A^CATGT
BstBI 1580 TTCGAA TT^CGAA
DrdI 4405 GACCCCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 419 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 5267 GATATC GAT^ATC
KpnI 366 GGTACC GGTAC^C
NruI 2970 TCGCGA TCG^CGA
NspV 1580 TTCGAA TT^CGAA
PinAI 5663 ACCGGT A^CCGGT
PmaCI 3916 CACGTG CAC^GTG
PmlI 3916 CACGTG CAC^GTG
SapI 5071 GAAGAGC GCTCTTC(1/4)
SbfI 5761 CCTGCAGG CCTGCA^GG
SfuI 1580 TTCGAA TT^CGAA
SphI 3770 GCATGC GCATG^C
Sse8387I 5761 CCTGCAGG CCTGCA^GG
TfiI 3701 GAATC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 2230 TCCGGA T^CCGGA
3842 TCCGGA
Alw44I 3523 GTGCAC G^TGCAC
4366 GTGCAC
ApaLI 3523 GTGCAC G^TGCAC
4366 GTGCAC
AvrII 3109 CCTAGG C^CTAGG
3814 CCTAGG
BclI 453 TGATCA T^GATCA
1907 TGATCA
BlnI 3109 CCTAGG C^CTAGG
3814 CCTAGG
BlpI 841 GCTCAGC GC^T[ACGT]AGC
4041 GCTCAGC
Bpu1102I 841 GCTCAGC GC^T[ACGT]AGC
4041 GCTCAGC
BseAI 2230 TCCGGA T^CCGGA
3842 TCCGGA
BspEI 2230 TCCGGA T^CCGGA
3842 TCCGGA
BspHI 153 TCATGA T^CATGA
510 TCATGA
BsrDI 3985 CATTGC GCAATG(2/0)
5792 GCAATG
BssHII 3085 GCGCGC G^CGCGC
4154 GCGCGC
BstAPI 547 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4738 GCACCTGCTGC
BstXI 1444 CCAGGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1536 CCACAAAGCTGG
CelII 841 GCTCAGC GC^T[ACGT]AGC
4041 GCTCAGC
EarI 3505 GAAGAG CTCTTC(1/4)
5071 GAAGAG
FbaI 453 TGATCA T^GATCA
1907 TGATCA
HincII 794 GTTGAC GT[CT]^[GA]AC
4519 GTCAAC
HindII 794 GTTGAC GT[CT]^[GA]AC
4519 GTCAAC
Ksp632I 3505 GAAGAG CTCTTC(1/4)
5071 GAAGAG
MroI 2230 TCCGGA T^CCGGA
3842 TCCGGA
MseI 1435 TTAA T^TAA
2555 TTAA
PshAI 3004 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3805 GACCCAGGTC
RcaI 153 TCATGA T^CATGA
510 TCATGA
SfiI 2716 GGCCGGGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
5759 GGCCTGCAGGGCC
Tru9I 1435 TTAA T^TAA
2555 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbeI BbsI BbvI
BcgI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BssSI BstEII BstF5I BstNI
BstPI BstUI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EagI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SacII Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SfoI SmaI SmlI
StuI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI