##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AclI AflII AgeI AhdI AscI
AspEI AspI AviII AvrII BbeI
BfrI BlnI BsiEI BsiWI BsmBI
BspDI Bst1107I BstZ17I ClaI CpoI
EagI Eam1105I EclXI Eco52I EcoRI
FseI FspI HpaI KasI KspI
MluI NarI NotI PacI PaeR7I
PflFI PinAI PmeI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SfiI SfoI SgrAI SnaBI
SphI Sse8387I SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
Alw44I 314 GTGCAC G^TGCAC
ApaI 4238 GGGCCC GGGCC^C
ApaLI 314 GTGCAC G^TGCAC
BamHI 3685 GGATCC G^GATCC
BbrPI 4246 CACGTG CAC^GTG
BbvCI 2518 CCTCAGC CCTCAGC(-5/-2)
BsaBI 3680 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 720 TCATGA T^CATGA
BstBI 4421 TTCGAA TT^CGAA
BstEII 1893 GGTCACC G^GT[ACGT]ACC
BstPI 1893 GGTCACC G^GT[ACGT]ACC
DrdI 276 GACAGGTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1893 GGTCACC G^GT[ACGT]ACC
EcoT22I 6366 ATGCAT ATGCA^T
MamI 3680 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1184 GCCGGC GCC^GGC
NdeI 4789 CATATG CA^TATG
NgoMIV 1184 GCCGGC G^CCGGC
NheI 1538 GCTAGC G^CTAGC
NruI 23 TCGCGA TCG^CGA
NsiI 6366 ATGCAT ATGCA^T
NspV 4421 TTCGAA TT^CGAA
PmaCI 4246 CACGTG CAC^GTG
PmlI 4246 CACGTG CAC^GTG
RcaI 720 TCATGA T^CATGA
SacI 69 GAGCTC GAGCT^C
SfuI 4421 TTCGAA TT^CGAA
SpeI 5174 ACTAGT A^CTAGT
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 2423 GACGTC GACGT^C
6617 GACGTC
Acc65I 4314 GGTACC G^GTACC
5731 GGTACC
AccI 1262 GTCTAC GT^[AC][GT]AC
4763 GTCTAC
AcyI 2423 GACGTC G[GA]^CG[CT]C
6617 GACGTC
AocI 3176 CCTCAGG CC^T[ACGT]AGG
4924 CCTGAGG
AseI 1031 ATTAAT AT^TAAT
1280 ATTAAT
AsnI 1031 ATTAAT AT^TAAT
1280 ATTAAT
Asp718I 4314 GGTACC G^GTACC
5731 GGTACC
BcgI 3913 CGATGAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
336 GCAACGTGATCG
BciVI 3655 GGATAC GTATCC(6/5)
3387 GTATCC
BclI 2469 TGATCA T^GATCA
6103 TGATCA
BsaHI 2423 GACGTC G[GA]^CG[CT]C
6617 GACGTC
BsmI 1467 GAATGC GAATGC(1/-1)
4424 GAATGC
BssHII 111 GCGCGC G^CGCGC
179 GCGCGC
BssSI 140 CTCGTG CACGAG(-5/-1)
2642 CTCGTG
BstAPI 91 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
6071 GCACCTGATGC
Bsu36I 3176 CCTCAGG CC^T[ACGT]AGG
4924 CCTGAGG
DraIII 1238 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
2735 CACCTGGTG
Eco81I 3176 CCTCAGG CC^T[ACGT]AGG
4924 CCTGAGG
EcoRV 5249 GATATC GAT^ATC
5309 GATATC
FbaI 2469 TGATCA T^GATCA
6103 TGATCA
HgaI 88 GACGC GACGC(5/10)
536 GACGC
Hin1I 2423 GACGTC G[GA]^CG[CT]C
6617 GACGTC
HindIII 2499 AAGCTT A^AGCTT
5284 AAGCTT
KpnI 4314 GGTACC GGTAC^C
5731 GGTACC
PshBI 1031 ATTAAT AT^TAAT
1280 ATTAAT
SapI 1771 GAAGAGC GCTCTTC(1/4)
5650 GAAGAGC
SexAI 1065 ACCAGGT A^CC[AT]GGT
2736 ACCTGGT
SmaI 852 CCCGGG CCC^GGG
2028 CCCGGG
SspI 1038 AATATT AAT^ATT
6674 AATATT
XmaCI 852 CCCGGG C^CCGGG
2028 CCCGGG
XmaI 852 CCCGGG C^CCGGG
2028 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccII AccIII AciI AcsI AfaI
AflIII AluI AlwI AlwNI Aor51HI
ApoI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco47III EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MunI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI