##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AclI AflII AgeI Alw44I ApaLI
AscI AseI AsnI AvrII BciVI
BclI BfrI BglII BlnI BsaBI
BsiWI BsrDI BssHII Bst1107I BstBI
BstZ17I DraI EcoRI EcoRV FbaI
HindIII HpaI MamI MfeI MunI
NdeI NotI NruI NspV PacI
PinAI PmeI PshBI Psp1406I PvuI
SalI ScaI SexAI SfuI SnaBI
SpeI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
Asp700I 6456 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2144 TGCGCA TGC^GCA
BbrPI 2071 CACGTG CAC^GTG
BbvCI 866 CCTCAGC CCTCAGC(-5/-2)
Bsp1407I 6658 TGTACA T^GTACA
BspHI 5543 TCATGA T^CATGA
BspLU11I 1778 ACATGT A^CATGT
BsrGI 6658 TGTACA T^GTACA
BssSI 4713 CTCGTG CACGAG(-5/-1)
CpoI 743 CGGTCCG CG^G[AT]CCG
DraIII 5783 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
FspI 2144 TGCGCA TGC^GCA
KspI 960 CCGCGG CCGC^GG
MluI 4274 ACGCGT A^CGCGT
MseI 6704 TTAA T^TAA
PmaCI 2071 CACGTG CAC^GTG
PmlI 2071 CACGTG CAC^GTG
RcaI 5543 TCATGA T^CATGA
RsrII 743 CGGTCCG CG^G[AT]CCG
SacII 960 CCGCGG CCGC^GG
SbfI 505 CCTGCAGG CCTGCA^GG
SgrAI 692 CGCCGGCG C[GA]^CCGG[CT]G
SphI 4116 GCATGC GCATG^C
Sse8387I 505 CCTGCAGG CCTGCA^GG
SspBI 6658 TGTACA T^GTACA
Tru9I 6704 TTAA T^TAA
XmnI 6456 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 2698 GGTACC G^GTACC
4621 GGTACC
AccI 1798 GTCTAC GT^[AC][GT]AC
4521 GTCTAC
AccIII 1840 TCCGGA T^CCGGA
2994 TCCGGA
AhdI 1600 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6179 GACCTCGAGTC
Asp718I 2698 GGTACC G^GTACC
4621 GGTACC
AspEI 1600 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6179 GACCTCGAGTC
AspI 739 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2332 GACACGGTC
BalI 1374 TGGCCA TGG^CCA
2036 TGGCCA
BamHI 258 GGATCC G^GATCC
3875 GGATCC
BcgI 5118 CGACACCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2483 GCAGCACCTTCG
BsaAI 2071 CACGTG [CT]AC^GT[GA]
2344 TACGTG
BsaWI 1840 TCCGGA [AT]^CCGG[AT]
2994 TCCGGA
BseAI 1840 TCCGGA T^CCGGA
2994 TCCGGA
BsmI 751 GAATGC GAATGC(1/-1)
2515 GAATGC
BspDI 5227 ATCGAT AT^CGAT
6714 ATCGAT
BspEI 1840 TCCGGA T^CCGGA
2994 TCCGGA
ClaI 5227 ATCGAT AT^CGAT
6714 ATCGAT
Eam1105I 1600 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6179 GACCTCGAGTC
EcoT22I 2517 ATGCAT ATGCA^T
6710 ATGCAT
HincII 676 GTCAAC GT[CT]^[GA]AC
1972 GTCAAC
HindII 676 GTCAAC GT[CT]^[GA]AC
1972 GTCAAC
KpnI 2698 GGTACC GGTAC^C
4621 GGTACC
MluNI 1374 TGGCCA TGG^CCA
2036 TGGCCA
MroI 1840 TCCGGA T^CCGGA
2994 TCCGGA
MscI 1374 TGGCCA TGG^CCA
2036 TGGCCA
NheI 3133 GCTAGC G^CTAGC
3798 GCTAGC
NsiI 2517 ATGCAT ATGCA^T
6710 ATGCAT
PflFI 739 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2332 GACACGGTC
PshAI 4271 GACACGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5735 GACGGGAGTC
SapI 2866 GAAGAGC GCTCTTC(1/4)
1464 GCTCTTC
Tth111I 739 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2332 GACACGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 151 enzymes) #
#########################################
AatII AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI Aor51HI ApaI ApoI AspHI
AvaI AvaII BanI BanII BbeI
BbsI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DrdI DsaI EaeI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI FseI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PaeR7I
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfiI SfoI SmaI
SmlI StuI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoI XhoII XmaCI
XmaI