##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
Acc65I AclI AgeI AscI Asp718I
AvrII BbeI BbrPI BlnI BsiWI
BspLU11I BssHII Bst1107I BstBI BstZ17I
DrdI EagI EclXI Eco52I EcoRV
FseI HpaI KasI KpnI KspI
MfeI MluI MunI NarI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
Psp1406I SacII SalI SbfI SfiI
SfoI SfuI SnaBI SpeI Sse8387I
XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 3114 GACGTC GACGT^C
AccII 190 CGCG CG^CG
AccIII 1105 TCCGGA T^CCGGA
Aor51HI 6224 AGCGCT AGC^GCT
AspI 3153 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2740 GGATCC G^GATCC
BanI 3242 GGTGCC G^G[CT][GA]CC
BciVI 5875 GGATAC GTATCC(6/5)
BglII 1496 AGATCT A^GATCT
BsaAI 1376 TACGTG [CT]AC^GT[GA]
BseAI 1105 TCCGGA T^CCGGA
Bsp1407I 301 TGTACA T^GTACA
BspDI 4958 ATCGAT AT^CGAT
BspEI 1105 TCCGGA T^CCGGA
BsrGI 301 TGTACA T^GTACA
BssSI 5008 CACGAG CACGAG(-5/-1)
BstUI 190 CGCG CG^CG
ClaI 4958 ATCGAT AT^CGAT
CpoI 4605 CGGACCG CG^G[AT]CCG
DraIII 4556 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 6224 AGCGCT AGC^GCT
EcoT22I 2046 ATGCAT ATGCA^T
MroI 1105 TCCGGA T^CCGGA
MvnI 190 CGCG CG^CG
NaeI 4860 GCCGGC GCC^GGC
NgoMIV 4860 GCCGGC G^CCGGC
NsiI 2046 ATGCAT ATGCA^T
PflFI 3153 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 4431 CGATCG CGAT^CG
RsrII 4605 CGGACCG CG^G[AT]CCG
ScaI 155 AGTACT AGT^ACT
SexAI 2884 ACCAGGT A^CC[AT]GGT
SgrAI 4859 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 858 CCCGGG CCC^GGG
SphI 1886 GCATGC GCATG^C
SspBI 301 TGTACA T^GTACA
StuI 1510 AGGCCT AGG^CCT
SwaI 2143 ATTTAAAT ATTT^AAAT
Tth111I 3153 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 858 CCCGGG C^CCGGG
XmaI 858 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 769 GTCTAC GT^[AC][GT]AC
5992 GTAGAC
AflII 347 CTTAAG C^TTAAG
4999 CTTAAG
AflIII 2876 ACACGT A^C[GA][CT]GT
3782 ACACGT
AocI 617 CCTGAGG CC^T[ACGT]AGG
839 CCTAAGG
ApaI 1525 GGGCCC GGGCC^C
6315 GGGCCC
AseI 5769 ATTAAT AT^TAAT
5805 ATTAAT
AsnI 5769 ATTAAT AT^TAAT
5805 ATTAAT
BbsI 4832 GAAGAC GAAGAC(2/6)
2163 GTCTTC
BcnI 858 CCCGG CC^[GC]GG
859 CCGGG
BfrI 347 CTTAAG C^TTAAG
4999 CTTAAG
BglI 4007 GCCATACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
6268 GCCAGCTTGGC
BpuAI 4832 GAAGAC GAAGAC(2/6)
2163 GTCTTC
BsaWI 1105 TCCGGA [AT]^CCGG[AT]
4603 ACCGGA
BsmBI 4030 CGTCTC CGTCTC(1/5)
4890 GAGACG
BspHI 3195 TCATGA T^CATGA
6462 TCATGA
BstEII 2371 GGTGACC G^GT[ACGT]ACC
3949 GGTCACC
BstPI 2371 GGTGACC G^GT[ACGT]ACC
3949 GGTCACC
Bsu36I 617 CCTGAGG CC^T[ACGT]AGG
839 CCTAAGG
Eco81I 617 CCTGAGG CC^T[ACGT]AGG
839 CCTAAGG
EcoO65I 2371 GGTGACC G^GT[ACGT]ACC
3949 GGTCACC
EcoRI 1296 GAATTC G^AATTC
1781 GAATTC
HincII 1964 GTCAAC GT[CT]^[GA]AC
6121 GTCAAC
HindII 1964 GTCAAC GT[CT]^[GA]AC
6121 GTCAAC
NciI 858 CCCGG CC^[GC]GG
859 CCGGG
PflMI 2771 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3253 CCACGGTCTGG
PshAI 1466 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2084 GACCTTAGTC
PshBI 5769 ATTAAT AT^TAAT
5805 ATTAAT
RcaI 3195 TCATGA T^CATGA
6462 TCATGA
SspI 743 AATATT AAT^ATT
2012 AATATT
Van91I 2771 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3253 CCACGGTCTGG
XbaI 1199 TCTAGA T^CTAGA
2200 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AciI AcsI AcyI AfaI AhdI
AluI Alw44I AlwI AlwNI ApaLI
ApoI Asp700I AspEI AspHI AvaI
AvaII AviII BalI BanII BbvCI
BbvI BcgI BclI BfaI BlpI
BmyI BpmI Bpu1102I BsaBI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
Eam1105I EarI EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NcoI NdeI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmnI