################################## # NO CUT ENZYMES ( 51 enzymes) # ################################## Acc65I AclI AgeI AscI Asp718I AvrII BbeI BbrPI BlnI BsiWI BspLU11I BssHII Bst1107I BstBI BstZ17I DrdI EagI EclXI Eco52I EcoRV FseI HpaI KasI KpnI KspI MfeI MluI MunI NarI NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI Psp1406I SacII SalI SbfI SfiI SfoI SfuI SnaBI SpeI Sse8387I XhoI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 3114 GACGTC GACGT^C AccII 190 CGCG CG^CG AccIII 1105 TCCGGA T^CCGGA Aor51HI 6224 AGCGCT AGC^GCT AspI 3153 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 2740 GGATCC G^GATCC BanI 3242 GGTGCC G^G[CT][GA]CC BciVI 5875 GGATAC GTATCC(6/5) BglII 1496 AGATCT A^GATCT BsaAI 1376 TACGTG [CT]AC^GT[GA] BseAI 1105 TCCGGA T^CCGGA Bsp1407I 301 TGTACA T^GTACA BspDI 4958 ATCGAT AT^CGAT BspEI 1105 TCCGGA T^CCGGA BsrGI 301 TGTACA T^GTACA BssSI 5008 CACGAG CACGAG(-5/-1) BstUI 190 CGCG CG^CG ClaI 4958 ATCGAT AT^CGAT CpoI 4605 CGGACCG CG^G[AT]CCG DraIII 4556 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 6224 AGCGCT AGC^GCT EcoT22I 2046 ATGCAT ATGCA^T MroI 1105 TCCGGA T^CCGGA MvnI 190 CGCG CG^CG NaeI 4860 GCCGGC GCC^GGC NgoMIV 4860 GCCGGC G^CCGGC NsiI 2046 ATGCAT ATGCA^T PflFI 3153 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuI 4431 CGATCG CGAT^CG RsrII 4605 CGGACCG CG^G[AT]CCG ScaI 155 AGTACT AGT^ACT SexAI 2884 ACCAGGT A^CC[AT]GGT SgrAI 4859 CGCCGGCG C[GA]^CCGG[CT]G SmaI 858 CCCGGG CCC^GGG SphI 1886 GCATGC GCATG^C SspBI 301 TGTACA T^GTACA StuI 1510 AGGCCT AGG^CCT SwaI 2143 ATTTAAAT ATTT^AAAT Tth111I 3153 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 858 CCCGGG C^CCGGG XmaI 858 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccI 769 GTCTAC GT^[AC][GT]AC 5992 GTAGAC AflII 347 CTTAAG C^TTAAG 4999 CTTAAG AflIII 2876 ACACGT A^C[GA][CT]GT 3782 ACACGT AocI 617 CCTGAGG CC^T[ACGT]AGG 839 CCTAAGG ApaI 1525 GGGCCC GGGCC^C 6315 GGGCCC AseI 5769 ATTAAT AT^TAAT 5805 ATTAAT AsnI 5769 ATTAAT AT^TAAT 5805 ATTAAT BbsI 4832 GAAGAC GAAGAC(2/6) 2163 GTCTTC BcnI 858 CCCGG CC^[GC]GG 859 CCGGG BfrI 347 CTTAAG C^TTAAG 4999 CTTAAG BglI 4007 GCCATACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 6268 GCCAGCTTGGC BpuAI 4832 GAAGAC GAAGAC(2/6) 2163 GTCTTC BsaWI 1105 TCCGGA [AT]^CCGG[AT] 4603 ACCGGA BsmBI 4030 CGTCTC CGTCTC(1/5) 4890 GAGACG BspHI 3195 TCATGA T^CATGA 6462 TCATGA BstEII 2371 GGTGACC G^GT[ACGT]ACC 3949 GGTCACC BstPI 2371 GGTGACC G^GT[ACGT]ACC 3949 GGTCACC Bsu36I 617 CCTGAGG CC^T[ACGT]AGG 839 CCTAAGG Eco81I 617 CCTGAGG CC^T[ACGT]AGG 839 CCTAAGG EcoO65I 2371 GGTGACC G^GT[ACGT]ACC 3949 GGTCACC EcoRI 1296 GAATTC G^AATTC 1781 GAATTC HincII 1964 GTCAAC GT[CT]^[GA]AC 6121 GTCAAC HindII 1964 GTCAAC GT[CT]^[GA]AC 6121 GTCAAC NciI 858 CCCGG CC^[GC]GG 859 CCGGG PflMI 2771 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3253 CCACGGTCTGG PshAI 1466 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2084 GACCTTAGTC PshBI 5769 ATTAAT AT^TAAT 5805 ATTAAT RcaI 3195 TCATGA T^CATGA 6462 TCATGA SspI 743 AATATT AAT^ATT 2012 AATATT Van91I 2771 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3253 CCACGGTCTGG XbaI 1199 TCTAGA T^CTAGA 2200 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 135 enzymes) # ######################################### AciI AcsI AcyI AfaI AhdI AluI Alw44I AlwI AlwNI ApaLI ApoI Asp700I AspEI AspHI AvaI AvaII AviII BalI BanII BbvCI BbvI BcgI BclI BfaI BlpI BmyI BpmI Bpu1102I BsaBI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI Eam1105I EarI EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI