##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Alw44I ApaLI AscI AseI AsnI
Asp718I BciVI BclI BfrI BglII
BsiWI BsmI BspDI BspHI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI EcoO65I EcoRI EcoRV
EcoT22I FbaI HindIII HpaI KpnI
KspI MfeI MluI MunI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SapI
SbfI ScaI SfuI SgrAI SnaBI
Sse8387I SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccI 5088 GTCGAC GT^[AC][GT]AC
AccIII 5535 TCCGGA T^CCGGA
AhdI 2713 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1121 AGCGCT AGC^GCT
Asp700I 6394 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2713 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1636 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2334 CCTAGG C^CTAGG
BamHI 2097 GGATCC G^GATCC
BbrPI 2202 CACGTG CAC^GTG
BlnI 2334 CCTAGG C^CTAGG
BseAI 5535 TCCGGA T^CCGGA
Bsp1407I 6325 TGTACA T^GTACA
BspEI 5535 TCCGGA T^CCGGA
BsrGI 6325 TGTACA T^GTACA
DraI 4969 TTTAAA TTT^AAA
Eam1105I 2713 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1121 AGCGCT AGC^GCT
FseI 3331 GGCCGGCC GGCCGG^CC
HincII 5088 GTCGAC GT[CT]^[GA]AC
HindII 5088 GTCGAC GT[CT]^[GA]AC
MroI 5535 TCCGGA T^CCGGA
NheI 6084 GCTAGC G^CTAGC
PflFI 1636 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2202 CACGTG CAC^GTG
PmlI 2202 CACGTG CAC^GTG
PshAI 5510 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 5088 GTCGAC G^TCGAC
SexAI 1637 ACCAGGT A^CC[AT]GGT
SpeI 3418 ACTAGT A^CTAGT
SspBI 6325 TGTACA T^GTACA
Tth111I 1636 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 6394 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AcsI 4557 GAATTT [GA]^AATT[CT]
6394 GAATTT
ApoI 4557 GAATTT [GA]^AATT[CT]
6394 GAATTT
AviII 83 TGCGCA TGC^GCA
1955 TGCGCA
BcgI 839 CGAGCCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2439 CGACGGCCCTGC
BsaAI 2202 CACGTG [CT]AC^GT[GA]
5998 TACGTG
BsaBI 711 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4242 GATGAAGATC
BspLU11I 152 ACATGT A^CATGT
4747 ACATGT
BstAPI 3433 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3832 GCAGAGATTGC
DrdI 892 GACCTGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5091 GACTGTGGGGTC
FspI 83 TGCGCA TGC^GCA
1955 TGCGCA
MamI 711 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4242 GATGAAGATC
MseI 4970 TTAA T^TAA
6216 TTAA
SphI 1958 GCATGC GCATG^C
6118 GCATGC
TfiI 145 GATTC G^A[AT]TC
5649 GATTC
Tru9I 4970 TTAA T^TAA
6216 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 145 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EagI EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfiI SfoI
SmaI SmlI StuI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI