##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AclI AflII AgeI Aor51HI Asp700I
AspI BclI BfrI BglII BsaBI
BsiWI BsmI BspDI BspLU11I Bst1107I
BstBI BstZ17I ClaI CpoI DraI
Eco47III EcoRV EcoT22I FbaI HpaI
KspI MamI MluI NdeI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI Psp1406I
PvuI RsrII SacII ScaI SfuI
SnaBI SpeI SphI SspI SwaI
Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 23 enzymes) #
###################################
AccIII 1333 TCCGGA T^CCGGA
AscI 1424 GGCGCGCC GG^CGCGCC
AseI 6282 ATTAAT AT^TAAT
AsnI 6282 ATTAAT AT^TAAT
BciVI 3 GGATAC GTATCC(6/5)
BsaWI 1333 TCCGGA [AT]^CCGG[AT]
BseAI 1333 TCCGGA T^CCGGA
BspEI 1333 TCCGGA T^CCGGA
BspHI 3458 TCATGA T^CATGA
BsrDI 1716 CATTGC GCAATG(2/0)
BssSI 684 CACGAG CACGAG(-5/-1)
HindIII 5835 AAGCTT A^AGCTT
MfeI 6073 CAATTG C^AATTG
MroI 1333 TCCGGA T^CCGGA
MunI 6073 CAATTG C^AATTG
NheI 5182 GCTAGC G^CTAGC
PshBI 6282 ATTAAT AT^TAAT
RcaI 3458 TCATGA T^CATGA
SalI 4328 GTCGAC G^TCGAC
SexAI 5333 ACCAGGT A^CC[AT]GGT
SgrAI 3728 CGCCGGTG C[GA]^CCGG[CT]G
TfiI 6131 GAATC G^A[AT]TC
XbaI 5798 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 2276 GAATTC [GA]^AATT[CT]
3315 GAATTC
ApoI 2276 GAATTC [GA]^AATT[CT]
3315 GAATTC
BbeI 3231 GGCGCC GGCGC^C
4334 GGCGCC
Bsp1407I 6214 TGTACA T^GTACA
6276 TGTACA
BsrBI 528 GAGCGG CCGCTC(-3/-3)
5163 GAGCGG
BsrGI 6214 TGTACA T^GTACA
6276 TGTACA
BstXI 749 CCAGTCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5776 CCATCCCTGTGG
DraIII 6086 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
6123 CACTGTGTG
DrdI 1878 GACAGTTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5597 GACGAGGCCGTC
EagI 874 CGGCCG C^GGCCG
1487 CGGCCG
EclXI 874 CGGCCG C^GGCCG
1487 CGGCCG
Eco52I 874 CGGCCG C^GGCCG
1487 CGGCCG
EcoRI 2276 GAATTC G^AATTC
3315 GAATTC
FseI 875 GGCCGGCC GGCCGG^CC
1319 GGCCGGCC
HincII 198 GTCAAC GT[CT]^[GA]AC
4328 GTCGAC
HindII 198 GTCAAC GT[CT]^[GA]AC
4328 GTCGAC
KasI 3231 GGCGCC G^GCGCC
4334 GGCGCC
NarI 3231 GGCGCC GG^CGCC
4334 GGCGCC
PshAI 1294 GACCCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5319 GACCTCAGTC
SapI 1133 GAAGAGC GCTCTTC(1/4)
4512 GCTCTTC
SbfI 5260 CCTGCAGG CCTGCA^GG
6057 CCTGCAGG
SfiI 586 GGCCCCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
6055 GGCCTGCAGGGCC
SfoI 3231 GGCGCC GGC^GCC
4334 GGCGCC
Sse8387I 5260 CCTGCAGG CCTGCA^GG
6057 CCTGCAGG
SspBI 6214 TGTACA T^GTACA
6276 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 157 enzymes) #
#########################################
AatII Acc65I AccI AccII AciI
AcyI AfaI AflIII AhdI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI Asp718I AspEI AspHI AvaI
AvaII AviII AvrII BalI BamHI
BanI BanII BbrPI BbsI BbvCI
BbvI BcgI BcnI BfaI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssHII
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI Eam1105I
EarI Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI