################################## # NO CUT ENZYMES ( 53 enzymes) # ################################## AclI AflII AgeI Aor51HI Asp700I AspI BclI BfrI BglII BsaBI BsiWI BsmI BspDI BspLU11I Bst1107I BstBI BstZ17I ClaI CpoI DraI Eco47III EcoRV EcoT22I FbaI HpaI KspI MamI MluI NdeI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI Psp1406I PvuI RsrII SacII ScaI SfuI SnaBI SpeI SphI SspI SwaI Tth111I XhoI XmnI ################################### # ONE CUT ENZYMES ( 23 enzymes) # ################################### AccIII 1333 TCCGGA T^CCGGA AscI 1424 GGCGCGCC GG^CGCGCC AseI 6282 ATTAAT AT^TAAT AsnI 6282 ATTAAT AT^TAAT BciVI 3 GGATAC GTATCC(6/5) BsaWI 1333 TCCGGA [AT]^CCGG[AT] BseAI 1333 TCCGGA T^CCGGA BspEI 1333 TCCGGA T^CCGGA BspHI 3458 TCATGA T^CATGA BsrDI 1716 CATTGC GCAATG(2/0) BssSI 684 CACGAG CACGAG(-5/-1) HindIII 5835 AAGCTT A^AGCTT MfeI 6073 CAATTG C^AATTG MroI 1333 TCCGGA T^CCGGA MunI 6073 CAATTG C^AATTG NheI 5182 GCTAGC G^CTAGC PshBI 6282 ATTAAT AT^TAAT RcaI 3458 TCATGA T^CATGA SalI 4328 GTCGAC G^TCGAC SexAI 5333 ACCAGGT A^CC[AT]GGT SgrAI 3728 CGCCGGTG C[GA]^CCGG[CT]G TfiI 6131 GAATC G^A[AT]TC XbaI 5798 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcsI 2276 GAATTC [GA]^AATT[CT] 3315 GAATTC ApoI 2276 GAATTC [GA]^AATT[CT] 3315 GAATTC BbeI 3231 GGCGCC GGCGC^C 4334 GGCGCC Bsp1407I 6214 TGTACA T^GTACA 6276 TGTACA BsrBI 528 GAGCGG CCGCTC(-3/-3) 5163 GAGCGG BsrGI 6214 TGTACA T^GTACA 6276 TGTACA BstXI 749 CCAGTCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5776 CCATCCCTGTGG DraIII 6086 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG 6123 CACTGTGTG DrdI 1878 GACAGTTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5597 GACGAGGCCGTC EagI 874 CGGCCG C^GGCCG 1487 CGGCCG EclXI 874 CGGCCG C^GGCCG 1487 CGGCCG Eco52I 874 CGGCCG C^GGCCG 1487 CGGCCG EcoRI 2276 GAATTC G^AATTC 3315 GAATTC FseI 875 GGCCGGCC GGCCGG^CC 1319 GGCCGGCC HincII 198 GTCAAC GT[CT]^[GA]AC 4328 GTCGAC HindII 198 GTCAAC GT[CT]^[GA]AC 4328 GTCGAC KasI 3231 GGCGCC G^GCGCC 4334 GGCGCC NarI 3231 GGCGCC GG^CGCC 4334 GGCGCC PshAI 1294 GACCCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 5319 GACCTCAGTC SapI 1133 GAAGAGC GCTCTTC(1/4) 4512 GCTCTTC SbfI 5260 CCTGCAGG CCTGCA^GG 6057 CCTGCAGG SfiI 586 GGCCCCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 6055 GGCCTGCAGGGCC SfoI 3231 GGCGCC GGC^GCC 4334 GGCGCC Sse8387I 5260 CCTGCAGG CCTGCA^GG 6057 CCTGCAGG SspBI 6214 TGTACA T^GTACA 6276 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 157 enzymes) # ######################################### AatII Acc65I AccI AccII AciI AcyI AfaI AflIII AhdI AluI Alw44I AlwI AlwNI AocI ApaI ApaLI Asp718I AspEI AspHI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbrPI BbsI BbvCI BbvI BcgI BcnI BfaI BglI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssHII BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI