##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
AspEI AviII AvrII BamHI BcgI
BciVI BfrI BlnI BsaWI BseAI
BsiWI BspDI BspEI BsrBI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI Eam1105I Eco47III EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HindIII HpaI MfeI MroI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RsrII SalI
SapI SbfI ScaI SexAI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 3208 GGTACC G^GTACC
ApaI 2971 GGGCCC GGGCC^C
Asp718I 3208 GGTACC G^GTACC
BbeI 2241 GGCGCC GGCGC^C
BbrPI 3071 CACGTG CAC^GTG
BglII 3381 AGATCT A^GATCT
BsaAI 3071 CACGTG [CT]AC^GT[GA]
BsaBI 1445 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 4065 TCATGA T^CATGA
BssHII 4617 GCGCGC G^CGCGC
EagI 6236 CGGCCG C^GGCCG
EclXI 6236 CGGCCG C^GGCCG
Eco52I 6236 CGGCCG C^GGCCG
HaeII 2241 GGCGCC [GA]GCGC^[CT]
HincII 2855 GTTGAC GT[CT]^[GA]AC
HindII 2855 GTTGAC GT[CT]^[GA]AC
KasI 2241 GGCGCC G^GCGCC
KpnI 3208 GGTACC GGTAC^C
MamI 1445 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1074 ACGCGT A^CGCGT
NaeI 3004 GCCGGC GCC^GGC
NarI 2241 GGCGCC GG^CGCC
NgoMIV 3004 GCCGGC G^CCGGC
PmaCI 3071 CACGTG CAC^GTG
PmlI 3071 CACGTG CAC^GTG
RcaI 4065 TCATGA T^CATGA
SfoI 2241 GGCGCC GGC^GCC
SmaI 3790 CCCGGG CCC^GGG
SspI 1582 AATATT AAT^ATT
StuI 115 AGGCCT AGG^CCT
SwaI 1446 ATTTAAAT ATTT^AAAT
XmaCI 3790 CCCGGG C^CCGGG
XmaI 3790 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AatII 212 GACGTC GACGT^C
3366 GACGTC
AlwNI 5434 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
5541 CAGTTTCTG
Asp700I 2712 GAATGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5974 GAAATGGTTC
BbsI 4147 GAAGAC GAAGAC(2/6)
5720 GTCTTC
BpuAI 4147 GAAGAC GAAGAC(2/6)
5720 GTCTTC
BsiEI 4731 CGGTCG CG[GA][CT]^CG
6236 CGGCCG
BspLU11I 2742 ACATGT A^CATGT
4806 ACATGT
BsrDI 3339 CATTGC GCAATG(2/0)
5176 CATTGC
BstAPI 3633 GCAGTTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3777 GCAACCTCTGC
EarI 1638 CTCTTC CTCTTC(1/4)
339 GAAGAG
Ksp632I 1638 CTCTTC CTCTTC(1/4)
339 GAAGAG
PshAI 2654 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4504 GACTGTGGTC
SfiI 5125 GGCCTTCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
5859 GGCCGGGTTGGCC
XmnI 2712 GAATGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5974 GAAATGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AocI
ApaLI ApoI AspHI AspI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BclI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspMI
BsrFI BsrGI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DraIII DrdI DsaI EaeI Eco81I
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SacI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII