################################## # NO CUT ENZYMES ( 46 enzymes) # ################################## AatII AccI AclI AflII AgeI AscI AseI AsnI Asp700I BciVI BfrI BglII BsaBI BspDI Bst1107I BstBI BstZ17I ClaI CpoI DraI DrdI EcoT22I HindIII MamI MfeI MunI NheI NotI NruI NsiI NspV PacI PinAI PmeI PshBI Psp1406I PvuI RsrII SalI SapI ScaI SfuI SpeI SspI SwaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccIII 193 TCCGGA T^CCGGA AcsI 5897 GAATTC [GA]^AATT[CT] AhdI 372 GACCGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 4584 GTGCAC G^TGCAC Aor51HI 115 AGCGCT AGC^GCT ApaLI 4584 GTGCAC G^TGCAC ApoI 5897 GAATTC [GA]^AATT[CT] AspEI 372 GACCGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1367 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BclI 5912 TGATCA T^GATCA BlpI 3002 GCTGAGC GC^T[ACGT]AGC Bpu1102I 3002 GCTGAGC GC^T[ACGT]AGC BseAI 193 TCCGGA T^CCGGA BsiWI 676 CGTACG C^GTACG Bsp1407I 4422 TGTACA T^GTACA BspEI 193 TCCGGA T^CCGGA BspHI 5517 TCATGA T^CATGA BspLU11I 4688 ACATGT A^CATGT BsrDI 3802 CATTGC GCAATG(2/0) BsrGI 4422 TGTACA T^GTACA CelII 3002 GCTGAGC GC^T[ACGT]AGC EagI 1698 CGGCCG C^GGCCG Eam1105I 372 GACCGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1698 CGGCCG C^GGCCG Eco47III 115 AGCGCT AGC^GCT Eco52I 1698 CGGCCG C^GGCCG EcoRI 5897 GAATTC G^AATTC EcoRV 2760 GATATC GAT^ATC FbaI 5912 TGATCA T^GATCA FseI 1699 GGCCGGCC GGCCGG^CC HincII 6336 GTTAAC GT[CT]^[GA]AC HindII 6336 GTTAAC GT[CT]^[GA]AC HpaI 6336 GTTAAC GTT^AAC MluI 223 ACGCGT A^CGCGT MroI 193 TCCGGA T^CCGGA PflFI 1367 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 2588 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 5517 TCATGA T^CATGA SbfI 5183 CCTGCAGG CCTGCA^GG SexAI 4933 ACCAGGT A^CC[AT]GGT SfiI 4748 GGCCCCACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 1765 CACCGGCG C[GA]^CCGG[CT]G Sse8387I 5183 CCTGCAGG CCTGCA^GG SspBI 4422 TGTACA T^GTACA Tth111I 1367 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 5996 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### Acc65I 472 GGTACC G^GTACC 872 GGTACC Asp718I 472 GGTACC G^GTACC 872 GGTACC AviII 2035 TGCGCA TGC^GCA 4455 TGCGCA BsaWI 193 TCCGGA [AT]^CCGG[AT] 3963 ACCGGA BsmI 6181 GAATGC GAATGC(1/-1) 5164 GCATTC DraIII 4727 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG 4729 CACCGTGTG FspI 2035 TGCGCA TGC^GCA 4455 TGCGCA KpnI 472 GGTACC GGTAC^C 872 GGTACC KspI 1632 CCGCGG CCGC^GG 2202 CCGCGG PaeR7I 119 CTCGAG C^TCGAG 3920 CTCGAG SacII 1632 CCGCGG CCGC^GG 2202 CCGCGG SmaI 1417 CCCGGG CCC^GGG 1905 CCCGGG SnaBI 5757 TACGTA TAC^GTA 6062 TACGTA TfiI 2219 GAATC G^A[AT]TC 3749 GATTC XhoI 119 CTCGAG C^TCGAG 3920 CTCGAG XmaCI 1417 CCCGGG C^CCGGG 1905 CCCGGG XmaI 1417 CCCGGG C^CCGGG 1905 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 149 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BcnI BfaI BglI BlnI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssHII BssKI BssSI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI SphI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII