##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
AatII AccI AclI AflII AgeI
AscI AseI AsnI Asp700I BciVI
BfrI BglII BsaBI BspDI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DrdI EcoT22I HindIII MamI MfeI
MunI NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshBI
Psp1406I PvuI RsrII SalI SapI
ScaI SfuI SpeI SspI SwaI
XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 193 TCCGGA T^CCGGA
AcsI 5897 GAATTC [GA]^AATT[CT]
AhdI 372 GACCGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4584 GTGCAC G^TGCAC
Aor51HI 115 AGCGCT AGC^GCT
ApaLI 4584 GTGCAC G^TGCAC
ApoI 5897 GAATTC [GA]^AATT[CT]
AspEI 372 GACCGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1367 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 5912 TGATCA T^GATCA
BlpI 3002 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3002 GCTGAGC GC^T[ACGT]AGC
BseAI 193 TCCGGA T^CCGGA
BsiWI 676 CGTACG C^GTACG
Bsp1407I 4422 TGTACA T^GTACA
BspEI 193 TCCGGA T^CCGGA
BspHI 5517 TCATGA T^CATGA
BspLU11I 4688 ACATGT A^CATGT
BsrDI 3802 CATTGC GCAATG(2/0)
BsrGI 4422 TGTACA T^GTACA
CelII 3002 GCTGAGC GC^T[ACGT]AGC
EagI 1698 CGGCCG C^GGCCG
Eam1105I 372 GACCGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1698 CGGCCG C^GGCCG
Eco47III 115 AGCGCT AGC^GCT
Eco52I 1698 CGGCCG C^GGCCG
EcoRI 5897 GAATTC G^AATTC
EcoRV 2760 GATATC GAT^ATC
FbaI 5912 TGATCA T^GATCA
FseI 1699 GGCCGGCC GGCCGG^CC
HincII 6336 GTTAAC GT[CT]^[GA]AC
HindII 6336 GTTAAC GT[CT]^[GA]AC
HpaI 6336 GTTAAC GTT^AAC
MluI 223 ACGCGT A^CGCGT
MroI 193 TCCGGA T^CCGGA
PflFI 1367 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2588 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 5517 TCATGA T^CATGA
SbfI 5183 CCTGCAGG CCTGCA^GG
SexAI 4933 ACCAGGT A^CC[AT]GGT
SfiI 4748 GGCCCCACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1765 CACCGGCG C[GA]^CCGG[CT]G
Sse8387I 5183 CCTGCAGG CCTGCA^GG
SspBI 4422 TGTACA T^GTACA
Tth111I 1367 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5996 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
Acc65I 472 GGTACC G^GTACC
872 GGTACC
Asp718I 472 GGTACC G^GTACC
872 GGTACC
AviII 2035 TGCGCA TGC^GCA
4455 TGCGCA
BsaWI 193 TCCGGA [AT]^CCGG[AT]
3963 ACCGGA
BsmI 6181 GAATGC GAATGC(1/-1)
5164 GCATTC
DraIII 4727 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
4729 CACCGTGTG
FspI 2035 TGCGCA TGC^GCA
4455 TGCGCA
KpnI 472 GGTACC GGTAC^C
872 GGTACC
KspI 1632 CCGCGG CCGC^GG
2202 CCGCGG
PaeR7I 119 CTCGAG C^TCGAG
3920 CTCGAG
SacII 1632 CCGCGG CCGC^GG
2202 CCGCGG
SmaI 1417 CCCGGG CCC^GGG
1905 CCCGGG
SnaBI 5757 TACGTA TAC^GTA
6062 TACGTA
TfiI 2219 GAATC G^A[AT]TC
3749 GATTC
XhoI 119 CTCGAG C^TCGAG
3920 CTCGAG
XmaCI 1417 CCCGGG C^CCGGG
1905 CCCGGG
XmaI 1417 CCCGGG C^CCGGG
1905 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 149 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BbvI BcgI BcnI
BfaI BglI BlnI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssHII BssKI BssSI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI SphI StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII