##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AseI AsnI Asp718I AspI
AvrII BciVI BfrI BlnI BsiWI
Bsp1407I BspDI BsrGI Bst1107I BstBI
BstZ17I ClaI CpoI DraI DrdI
EcoRI EcoRV EcoT22I FseI HindIII
HpaI KpnI KspI MfeI MluI
MunI NdeI NheI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI Tth111I
XhoI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AhdI 5153 GACGAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 5278 GTGCAC G^TGCAC
Aor51HI 2903 AGCGCT AGC^GCT
ApaI 5701 GGGCCC GGGCC^C
ApaLI 5278 GTGCAC G^TGCAC
AscI 3126 GGCGCGCC GG^CGCGCC
AspEI 5153 GACGAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 822 GGATCC G^GATCC
BclI 1017 TGATCA T^GATCA
BglII 5583 AGATCT A^GATCT
BsmBI 4343 GAGACG CGTCTC(1/5)
BsmI 3863 GCATTC GAATGC(1/-1)
BspHI 1677 TCATGA T^CATGA
BspLU11I 1200 ACATGT A^CATGT
DraIII 2873 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 5153 GACGAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2903 AGCGCT AGC^GCT
FbaI 1017 TGATCA T^GATCA
NotI 1 GCGGCCGC GC^GGCCGC
PshAI 3340 GACCTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1677 TCATGA T^CATGA
SbfI 3787 CCTGCAGG CCTGCA^GG
ScaI 2088 AGTACT AGT^ACT
Sse8387I 3787 CCTGCAGG CCTGCA^GG
XbaI 522 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccIII 2349 TCCGGA T^CCGGA
4554 TCCGGA
AflIII 1200 ACATGT A^C[GA][CT]GT
4278 ACGTGT
BbeI 161 GGCGCC GGCGC^C
5187 GGCGCC
BsaBI 823 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2125 GATCAAGATC
BsaWI 2349 TCCGGA [AT]^CCGG[AT]
4554 TCCGGA
BseAI 2349 TCCGGA T^CCGGA
4554 TCCGGA
BspEI 2349 TCCGGA T^CCGGA
4554 TCCGGA
BssHII 139 GCGCGC G^CGCGC
3127 GCGCGC
HincII 1859 GTCAAC GT[CT]^[GA]AC
3650 GTCAAC
HindII 1859 GTCAAC GT[CT]^[GA]AC
3650 GTCAAC
KasI 161 GGCGCC G^GCGCC
5187 GGCGCC
MamI 823 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2125 GATCAAGATC
MroI 2349 TCCGGA T^CCGGA
4554 TCCGGA
NarI 161 GGCGCC GG^CGCC
5187 GGCGCC
SacI 3383 GAGCTC GAGCT^C
4691 GAGCTC
SexAI 1937 ACCTGGT A^CC[AT]GGT
2112 ACCTGGT
SfoI 161 GGCGCC GGC^GCC
5187 GGCGCC
SphI 1357 GCATGC GCATG^C
5839 GCATGC
StuI 4032 AGGCCT AGG^CCT
5421 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 153 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaI AvaII AviII
BalI BanI BanII BbrPI BbsI
BbvCI BbvI BcgI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BssSI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfiI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI XmnI