##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccIII AclI AgeI AocI
Aor51HI ApaI AscI AseI AsnI
AviII AvrII BbvCI BcgI BlnI
BlpI Bpu1102I BsaWI BseAI BsiEI
BsiWI BsmBI BspEI BsrBI BsrFI
BssSI BstBI BstXI Bsu36I CelII
Cfr10I CpoI DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoRV FseI
FspI KspI MluI MroI NaeI
NgoMIV NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfiI SfuI SgrAI
SnaBI Sse8387I StuI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AflII 4306 CTTAAG C^TTAAG
AspI 3557 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 184 CCCGGG C^[CT]CG[GA]G
BamHI 2676 GGATCC G^GATCC
BbeI 125 GGCGCC GGCGC^C
BbrPI 3156 CACGTG CAC^GTG
BclI 2341 TGATCA T^GATCA
BfrI 4306 CTTAAG C^TTAAG
BglI 151 GCCCGCTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 214 GAGACC GGTCTC(1/5)
BsoBI 184 CCCGGG C^[CT]CG[GA]G
BspDI 636 ATCGAT AT^CGAT
BsrDI 5944 CATTGC GCAATG(2/0)
BssHII 4612 GCGCGC G^CGCGC
BstAPI 6215 GCACAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 636 ATCGAT AT^CGAT
EcoNI 1862 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 2341 TGATCA T^GATCA
HpaI 3946 GTTAAC GTT^AAC
KasI 125 GGCGCC G^GCGCC
MfeI 2325 CAATTG C^AATTG
MunI 2325 CAATTG C^AATTG
NarI 125 GGCGCC GG^CGCC
PflFI 3557 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3156 CACGTG CAC^GTG
PmlI 3156 CACGTG CAC^GTG
PpuMI 1759 GGGACCT [GA]G^G[AT]CC[CT]
SacI 5060 GAGCTC GAGCT^C
SexAI 1279 ACCTGGT A^CC[AT]GGT
SfoI 125 GGCGCC GGC^GCC
SmaI 184 CCCGGG CCC^GGG
SpeI 6184 ACTAGT A^CTAGT
SphI 147 GCATGC GCATG^C
Tth111I 3557 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 184 CCCGGG C^CCGGG
XmaI 184 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 1421 GGTACC G^GTACC
3640 GGTACC
AccII 201 CGCG CG^CG
4613 CGCG
AcyI 125 GGCGCC G[GA]^CG[CT]C
6486 GGCGTC
Alw44I 1905 GTGCAC G^TGCAC
4717 GTGCAC
ApaLI 1905 GTGCAC G^TGCAC
4717 GTGCAC
Asp718I 1421 GGTACC G^GTACC
3640 GGTACC
BanII 457 GAGCCC G[GA]GC[CT]^C
5060 GAGCTC
BciVI 3570 GGATAC GTATCC(6/5)
1827 GTATCC
BsaBI 692 GATAGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2342 GATCATCATC
BsaHI 125 GGCGCC G[GA]^CG[CT]C
6486 GGCGTC
BspLU11I 324 ACATGT A^CATGT
2107 ACATGT
Bst1107I 513 GTATAC GTA^TAC
2401 GTATAC
BstEII 2924 GGTTACC G^GT[ACGT]ACC
4458 GGTAACC
BstPI 2924 GGTTACC G^GT[ACGT]ACC
4458 GGTAACC
BstUI 201 CGCG CG^CG
4613 CGCG
BstZ17I 513 GTATAC GTA^TAC
2401 GTATAC
DraII 1759 GGGACCT [GA]G^G[ACGT]CC[CT]
5717 AGGCCCT
EcoO109I 1759 GGGACCT [GA]G^G[ACGT]CC[CT]
5717 AGGCCCT
EcoO65I 2924 GGTTACC G^GT[ACGT]ACC
4458 GGTAACC
HaeII 125 GGCGCC [GA]GCGC^[CT]
141 GGCGCT
HgaI 1222 GCGTC GACGC(5/10)
6487 GCGTC
Hin1I 125 GGCGCC G[GA]^CG[CT]C
6486 GGCGTC
HincII 3946 GTTAAC GT[CT]^[GA]AC
4783 GTTGAC
HindII 3946 GTTAAC GT[CT]^[GA]AC
4783 GTTGAC
KpnI 1421 GGTACC GGTAC^C
3640 GGTACC
MamI 692 GATAGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2342 GATCATCATC
MvnI 201 CGCG CG^CG
4613 CGCG
NdeI 3926 CATATG CA^TATG
4363 CATATG
NheI 1490 GCTAGC G^CTAGC
1517 GCTAGC
PflMI 1571 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4681 CCATCCTTTGG
TaqI 637 TCGA T^CGA
700 TCGA
TthHB8I 637 TCGA T^CGA
700 TCGA
Van91I 1571 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4681 CCATCCTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AhdI AluI AlwI AlwNI ApoI
Asp700I AspEI AspHI AvaII BalI
BanI BbsI BbvI BcnI BfaI
BglII BmyI BpmI BpuAI BsaAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspHI BspMI BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraIII DsaI EaeI Eam1105I EarI
EcoRI EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NsiI NspI PleI
PstI PvuII RcaI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI XhoII XmnI