##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AatII AccI AccIII AgeI Aor51HI
AscI AspI AviII BcgI BciVI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BsrGI BssHII Bst1107I BstZ17I
ClaI CpoI EagI EclXI Eco47III
Eco52I FseI FspI HindIII KspI
MfeI MluI MroI MunI NotI
NruI PacI PflFI PinAI PmeI
PshAI PvuI RsrII SacII SalI
ScaI SgrAI SnaBI SspBI SwaI
Tth111I
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AflII 5676 CTTAAG C^TTAAG
AhdI 4031 GACTAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 4031 GACTAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 4704 TGGCCA TGG^CCA
BamHI 5492 GGATCC G^GATCC
BfrI 5676 CTTAAG C^TTAAG
BlpI 914 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 914 GCTTAGC GC^T[ACGT]AGC
BsaBI 3225 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspLU11I 2985 ACATGT A^CATGT
BsrFI 1705 GCCGGC [GA]^CCGG[CT]
CelII 914 GCTTAGC GC^T[ACGT]AGC
Cfr10I 1705 GCCGGC [GA]^CCGG[CT]
DrdI 160 GACTGCCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4031 GACTAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 228 GATATC GAT^ATC
EcoT22I 439 ATGCAT ATGCA^T
HincII 3610 GTTAAC GT[CT]^[GA]AC
HindII 3610 GTTAAC GT[CT]^[GA]AC
HpaI 3610 GTTAAC GTT^AAC
MamI 3225 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 4704 TGGCCA TGG^CCA
MscI 4704 TGGCCA TGG^CCA
NaeI 1705 GCCGGC GCC^GGC
NdeI 1883 CATATG CA^TATG
NgoMIV 1705 GCCGGC G^CCGGC
NheI 2671 GCTAGC G^CTAGC
NsiI 439 ATGCAT ATGCA^T
SacI 2175 GAGCTC GAGCT^C
SbfI 5560 CCTGCAGG CCTGCA^GG
SexAI 4769 ACCTGGT A^CC[AT]GGT
SfiI 1498 GGCCCTTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1736 CCCGGG CCC^GGG
Sse8387I 5560 CCTGCAGG CCTGCA^GG
XmaCI 1736 CCCGGG C^CCGGG
XmaI 1736 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AclI 3867 AACGTT AA^CGTT
4939 AACGTT
Alw44I 5135 GTGCAC G^TGCAC
5206 GTGCAC
AocI 890 CCTAAGG CC^T[ACGT]AGG
2907 CCTTAGG
ApaLI 5135 GTGCAC G^TGCAC
5206 GTGCAC
AseI 1313 ATTAAT AT^TAAT
3270 ATTAAT
AsnI 1313 ATTAAT AT^TAAT
3270 ATTAAT
AvrII 4510 CCTAGG C^CTAGG
5848 CCTAGG
BbvCI 106 GCTGAGG CCTCAGC(-5/-2)
1729 GCTGAGG
BglI 1499 GCCCTTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5505 GCCCCATGGGC
BglII 486 AGATCT A^GATCT
1928 AGATCT
BlnI 4510 CCTAGG C^CTAGG
5848 CCTAGG
BsaWI 5428 ACCGGA [AT]^CCGG[AT]
6405 ACCGGA
BspHI 2048 TCATGA T^CATGA
2424 TCATGA
BsrBI 139 CCGCTC CCGCTC(-3/-3)
375 CCGCTC
BsrDI 836 CATTGC GCAATG(2/0)
4680 CATTGC
BssSI 1513 CACGAG CACGAG(-5/-1)
4570 CTCGTG
BstBI 4397 TTCGAA TT^CGAA
6295 TTCGAA
BstEII 412 GGTTACC G^GT[ACGT]ACC
4257 GGTAACC
BstPI 412 GGTTACC G^GT[ACGT]ACC
4257 GGTAACC
BstXI 4858 CCAACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6137 CCATGAAAGTGG
Bsu36I 890 CCTAAGG CC^T[ACGT]AGG
2907 CCTTAGG
Eco81I 890 CCTAAGG CC^T[ACGT]AGG
2907 CCTTAGG
EcoNI 2489 CCTCCTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5647 CCTCCTGGAGG
EcoO65I 412 GGTTACC G^GT[ACGT]ACC
4257 GGTAACC
EcoRI 4345 GAATTC G^AATTC
4559 GAATTC
NspV 4397 TTCGAA TT^CGAA
6295 TTCGAA
PshBI 1313 ATTAAT AT^TAAT
3270 ATTAAT
Psp1406I 3867 AACGTT AA^CGTT
4939 AACGTT
RcaI 2048 TCATGA T^CATGA
2424 TCATGA
SapI 4813 GCTCTTC GCTCTTC(1/4)
5877 GCTCTTC
SfuI 4397 TTCGAA TT^CGAA
6295 TTCGAA
SpeI 2013 ACTAGT A^CTAGT
3614 ACTAGT
SphI 2066 GCATGC GCATG^C
2899 GCATGC
XbaI 1439 TCTAGA T^CTAGA
4071 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
Acc65I AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApaI ApoI Asp700I Asp718I AspHI
AvaI AvaII BanI BanII BbeI
BbrPI BbsI BbvI BclI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PaeR7I PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoI
XhoII XmnI