################################## # NO CUT ENZYMES ( 46 enzymes) # ################################## AccI AclI AhdI AocI AscI AseI AsnI AspEI AvrII BlnI BsiWI BsmI BspDI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI Eam1105I Eco81I FseI HpaI MluI NdeI NheI NotI NspV PacI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI SbfI SexAI SfiI SfuI SpeI Sse8387I SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### Acc65I 405 GGTACC G^GTACC AccIII 528 TCCGGA T^CCGGA AgeI 3892 ACCGGT A^CCGGT ApaI 3267 GGGCCC GGGCC^C Asp718I 405 GGTACC G^GTACC AspI 2880 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 3300 GGCGCC GGCGC^C BfaI 5460 CTAG C^TAG BlpI 5241 GCTAAGC GC^T[ACGT]AGC Bpu1102I 5241 GCTAAGC GC^T[ACGT]AGC BseAI 528 TCCGGA T^CCGGA BspEI 528 TCCGGA T^CCGGA BspHI 9 TCATGA T^CATGA BssHII 2757 GCGCGC G^CGCGC BstEII 1450 GGTCACC G^GT[ACGT]ACC BstPI 1450 GGTCACC G^GT[ACGT]ACC CelII 5241 GCTAAGC GC^T[ACGT]AGC EcoO65I 1450 GGTCACC G^GT[ACGT]ACC EcoRI 5004 GAATTC G^AATTC EcoT22I 773 ATGCAT ATGCA^T KasI 3300 GGCGCC G^GCGCC KpnI 405 GGTACC GGTAC^C KspI 3075 CCGCGG CCGC^GG MaeI 5460 CTAG C^TAG MfeI 5034 CAATTG C^AATTG MroI 528 TCCGGA T^CCGGA MunI 5034 CAATTG C^AATTG NarI 3300 GGCGCC GG^CGCC NruI 4133 TCGCGA TCG^CGA NsiI 773 ATGCAT ATGCA^T PflFI 2880 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 3892 ACCGGT A^CCGGT RcaI 9 TCATGA T^CATGA SacII 3075 CCGCGG CCGC^GG SfoI 3300 GGCGCC GGC^GCC SnaBI 5607 TACGTA TAC^GTA Tth111I 2880 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AflII 2276 CTTAAG C^TTAAG 4845 CTTAAG AviII 3499 TGCGCA TGC^GCA 4168 TGCGCA BciVI 3629 GTATCC GTATCC(6/5) 4879 GTATCC BfrI 2276 CTTAAG C^TTAAG 4845 CTTAAG BglI 2988 GCCCGTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 6231 GCCACGGCGGC BsaBI 3725 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 6101 GATGCAGATC BsiEI 3453 CGGCCG CG[GA][CT]^CG 4711 CGGCCG BsmBI 4090 CGTCTC CGTCTC(1/5) 68 GAGACG DraIII 1141 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG 1983 CACAAAGTG DrdI 2883 GACGTCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5810 GACCAACCTGTC EagI 3453 CGGCCG C^GGCCG 4711 CGGCCG EclXI 3453 CGGCCG C^GGCCG 4711 CGGCCG Eco52I 3453 CGGCCG C^GGCCG 4711 CGGCCG EcoRV 1978 GATATC GAT^ATC 4569 GATATC FspI 3499 TGCGCA TGC^GCA 4168 TGCGCA HindIII 2958 AAGCTT A^AGCTT 4254 AAGCTT MamI 3725 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 6101 GATGCAGATC PaeR7I 2007 CTCGAG C^TCGAG 6216 CTCGAG PflMI 410 CCAGACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1428 CCACAGGCTGG SacI 3459 GAGCTC GAGCT^C 6063 GAGCTC SgrAI 3296 CGCCGGCG C[GA]^CCGG[CT]G 4272 CGCCGGCG SphI 60 GCATGC GCATG^C 252 GCATGC StuI 2272 AGGCCT AGG^CCT 3397 AGGCCT Van91I 410 CCAGACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1428 CCACAGGCTGG XcmI 410 CCAGACATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4774 CCACCCTGAAGCTGG XhoI 2007 CTCGAG C^TCGAG 6216 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 149 enzymes) # ######################################### AatII AccII AciI AcsI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI Aor51HI ApaLI ApoI Asp700I AspHI AvaI AvaII BalI BamHI BanI BanII BbrPI BbsI BbvCI BbvI BcgI BclI BcnI BglII BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco47III EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmaI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI XmnI