##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
AccI AclI AhdI AocI AscI
AseI AsnI AspEI AvrII BlnI
BsiWI BsmI BspDI Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI DraI
Eam1105I Eco81I FseI HpaI MluI
NdeI NheI NotI NspV PacI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI SexAI SfiI
SfuI SpeI Sse8387I SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 405 GGTACC G^GTACC
AccIII 528 TCCGGA T^CCGGA
AgeI 3892 ACCGGT A^CCGGT
ApaI 3267 GGGCCC GGGCC^C
Asp718I 405 GGTACC G^GTACC
AspI 2880 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 3300 GGCGCC GGCGC^C
BfaI 5460 CTAG C^TAG
BlpI 5241 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 5241 GCTAAGC GC^T[ACGT]AGC
BseAI 528 TCCGGA T^CCGGA
BspEI 528 TCCGGA T^CCGGA
BspHI 9 TCATGA T^CATGA
BssHII 2757 GCGCGC G^CGCGC
BstEII 1450 GGTCACC G^GT[ACGT]ACC
BstPI 1450 GGTCACC G^GT[ACGT]ACC
CelII 5241 GCTAAGC GC^T[ACGT]AGC
EcoO65I 1450 GGTCACC G^GT[ACGT]ACC
EcoRI 5004 GAATTC G^AATTC
EcoT22I 773 ATGCAT ATGCA^T
KasI 3300 GGCGCC G^GCGCC
KpnI 405 GGTACC GGTAC^C
KspI 3075 CCGCGG CCGC^GG
MaeI 5460 CTAG C^TAG
MfeI 5034 CAATTG C^AATTG
MroI 528 TCCGGA T^CCGGA
MunI 5034 CAATTG C^AATTG
NarI 3300 GGCGCC GG^CGCC
NruI 4133 TCGCGA TCG^CGA
NsiI 773 ATGCAT ATGCA^T
PflFI 2880 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3892 ACCGGT A^CCGGT
RcaI 9 TCATGA T^CATGA
SacII 3075 CCGCGG CCGC^GG
SfoI 3300 GGCGCC GGC^GCC
SnaBI 5607 TACGTA TAC^GTA
Tth111I 2880 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflII 2276 CTTAAG C^TTAAG
4845 CTTAAG
AviII 3499 TGCGCA TGC^GCA
4168 TGCGCA
BciVI 3629 GTATCC GTATCC(6/5)
4879 GTATCC
BfrI 2276 CTTAAG C^TTAAG
4845 CTTAAG
BglI 2988 GCCCGTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
6231 GCCACGGCGGC
BsaBI 3725 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6101 GATGCAGATC
BsiEI 3453 CGGCCG CG[GA][CT]^CG
4711 CGGCCG
BsmBI 4090 CGTCTC CGTCTC(1/5)
68 GAGACG
DraIII 1141 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1983 CACAAAGTG
DrdI 2883 GACGTCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5810 GACCAACCTGTC
EagI 3453 CGGCCG C^GGCCG
4711 CGGCCG
EclXI 3453 CGGCCG C^GGCCG
4711 CGGCCG
Eco52I 3453 CGGCCG C^GGCCG
4711 CGGCCG
EcoRV 1978 GATATC GAT^ATC
4569 GATATC
FspI 3499 TGCGCA TGC^GCA
4168 TGCGCA
HindIII 2958 AAGCTT A^AGCTT
4254 AAGCTT
MamI 3725 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6101 GATGCAGATC
PaeR7I 2007 CTCGAG C^TCGAG
6216 CTCGAG
PflMI 410 CCAGACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1428 CCACAGGCTGG
SacI 3459 GAGCTC GAGCT^C
6063 GAGCTC
SgrAI 3296 CGCCGGCG C[GA]^CCGG[CT]G
4272 CGCCGGCG
SphI 60 GCATGC GCATG^C
252 GCATGC
StuI 2272 AGGCCT AGG^CCT
3397 AGGCCT
Van91I 410 CCAGACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1428 CCACAGGCTGG
XcmI 410 CCAGACATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4774 CCACCCTGAAGCTGG
XhoI 2007 CTCGAG C^TCGAG
6216 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 149 enzymes) #
#########################################
AatII AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI Aor51HI ApaLI ApoI Asp700I
AspHI AvaI AvaII BalI BamHI
BanI BanII BbrPI BbsI BbvCI
BbvI BcgI BclI BcnI BglII
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco47III EcoNI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmaI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmaCI XmaI XmnI