##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
AflII Alw44I Aor51HI ApaLI AscI
AseI AsnI AspI AviII AvrII
BciVI BfrI BlnI BspDI Bst1107I
BstBI BstZ17I ClaI CpoI Eco47III
EcoRV FseI FspI HindIII NheI
NruI NspV PacI PflFI PmeI
PshAI PshBI RsrII SalI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SwaI Tth111I XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 1551 GACGTC GACGT^C
Acc65I 4082 GGTACC G^GTACC
AccI 1554 GTCTAC GT^[AC][GT]AC
AclI 4682 AACGTT AA^CGTT
AgeI 3432 ACCGGT A^CCGGT
AocI 4501 CCTTAGG CC^T[ACGT]AGG
Asp718I 4082 GGTACC G^GTACC
BbrPI 588 CACGTG CAC^GTG
BcgI 2696 CGATGTGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 6473 AGATCT A^GATCT
BlpI 2443 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2443 GCTCAGC GC^T[ACGT]AGC
BsaBI 6283 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 268 CGTACG C^GTACG
BsmI 4611 GAATGC GAATGC(1/-1)
BspLU11I 5163 ACATGT A^CATGT
BsrBI 2172 GAGCGG CCGCTC(-3/-3)
BsrDI 5272 CATTGC GCAATG(2/0)
BssSI 4703 CTCGTG CACGAG(-5/-1)
BstEII 653 GGTCACC G^GT[ACGT]ACC
BstPI 653 GGTCACC G^GT[ACGT]ACC
Bsu36I 4501 CCTTAGG CC^T[ACGT]AGG
CelII 2443 GCTCAGC GC^T[ACGT]AGC
Eco81I 4501 CCTTAGG CC^T[ACGT]AGG
EcoO65I 653 GGTCACC G^GT[ACGT]ACC
HpaI 5171 GTTAAC GTT^AAC
KpnI 4082 GGTACC GGTAC^C
KspI 4207 CCGCGG CCGC^GG
MamI 6283 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2860 CAATTG C^AATTG
MluI 265 ACGCGT A^CGCGT
MunI 2860 CAATTG C^AATTG
NaeI 195 GCCGGC GCC^GGC
NdeI 2132 CATATG CA^TATG
NgoMIV 195 GCCGGC G^CCGGC
NotI 166 GCGGCCGC GC^GGCCGC
PinAI 3432 ACCGGT A^CCGGT
PmaCI 588 CACGTG CAC^GTG
PmlI 588 CACGTG CAC^GTG
Psp1406I 4682 AACGTT AA^CGTT
PvuI 2668 CGATCG CGAT^CG
SacII 4207 CCGCGG CCGC^GG
SbfI 4124 CCTGCAGG CCTGCA^GG
ScaI 1887 AGTACT AGT^ACT
Sse8387I 4124 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccIII 1678 TCCGGA T^CCGGA
1723 TCCGGA
BseAI 1678 TCCGGA T^CCGGA
1723 TCCGGA
BspEI 1678 TCCGGA T^CCGGA
1723 TCCGGA
BspHI 2240 TCATGA T^CATGA
4487 TCATGA
DrdI 510 GACCTGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2577 GACACCAAAGTC
EagI 167 CGGCCG C^GGCCG
2149 CGGCCG
EclXI 167 CGGCCG C^GGCCG
2149 CGGCCG
Eco52I 167 CGGCCG C^GGCCG
2149 CGGCCG
MroI 1678 TCCGGA T^CCGGA
1723 TCCGGA
NcoI 3485 CCATGG C^CATGG
5306 CCATGG
PaeR7I 1293 CTCGAG C^TCGAG
3931 CTCGAG
RcaI 2240 TCATGA T^CATGA
4487 TCATGA
SapI 4798 GCTCTTC GCTCTTC(1/4)
6568 GCTCTTC
SphI 1585 GCATGC GCATG^C
5276 GCATGC
SspI 1872 AATATT AAT^ATT
6661 AATATT
StuI 694 AGGCCT AGG^CCT
914 AGGCCT
XhoI 1293 CTCGAG C^TCGAG
3931 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 151 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
ApaI ApoI Asp700I AspEI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbeI BbsI BbvCI BbvI
BclI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrFI BsrGI BsrI BssHII BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI Eam1105I EarI EcoNI
EcoO109I EcoRI EcoRII EcoT14I EcoT22I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfoI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XhoII
XmnI