##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AclI AgeI AhdI Alw44I Aor51HI
ApaLI AscI AspEI AviII AvrII
BbrPI BcgI BlnI BsaBI BsaI
BsiWI Bsp1407I BspDI BsrGI BssHII
BstAPI BstBI ClaI CpoI DrdI
Eam1105I Eco47III EcoRV FseI FspI
HpaI KspI MamI MluI NaeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SfiI SfuI SgrAI
SpeI SphI SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 4749 GACGTC GACGT^C
AccIII 592 TCCGGA T^CCGGA
AflII 454 CTTAAG C^TTAAG
AspI 3885 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 5199 GGATCC G^GATCC
BbeI 4906 GGCGCC GGCGC^C
BciVI 5331 GGATAC GTATCC(6/5)
BfrI 454 CTTAAG C^TTAAG
BlpI 2429 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2429 GCTGAGC GC^T[ACGT]AGC
BsaAI 1058 TACGTA [CT]AC^GT[GA]
BsaWI 592 TCCGGA [AT]^CCGG[AT]
BseAI 592 TCCGGA T^CCGGA
BsgI 2324 GTGCAG GTGCAG(16/14)
BsiEI 3804 CGGCCG CG[GA][CT]^CG
BsmI 3385 GCATTC GAATGC(1/-1)
BspEI 592 TCCGGA T^CCGGA
BspHI 3796 TCATGA T^CATGA
BsrFI 2126 GCCGGT [GA]^CCGG[CT]
BssSI 727 CACGAG CACGAG(-5/-1)
BstEII 3985 GGTAACC G^GT[ACGT]ACC
BstPI 3985 GGTAACC G^GT[ACGT]ACC
CelII 2429 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2126 GCCGGT [GA]^CCGG[CT]
EagI 3804 CGGCCG C^GGCCG
EclXI 3804 CGGCCG C^GGCCG
Eco52I 3804 CGGCCG C^GGCCG
EcoO65I 3985 GGTAACC G^GT[ACGT]ACC
EcoRI 5537 GAATTC G^AATTC
EcoT22I 1868 ATGCAT ATGCA^T
HaeII 4906 GGCGCC [GA]GCGC^[CT]
HindIII 3284 AAGCTT A^AGCTT
KasI 4906 GGCGCC G^GCGCC
MfeI 4975 CAATTG C^AATTG
MroI 592 TCCGGA T^CCGGA
MunI 4975 CAATTG C^AATTG
NarI 4906 GGCGCC GG^CGCC
NsiI 1868 ATGCAT ATGCA^T
PflFI 3885 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 3752 CTGCAG CTGCA^G
RcaI 3796 TCATGA T^CATGA
SapI 683 GAAGAGC GCTCTTC(1/4)
SbfI 3751 CCTGCAGG CCTGCA^GG
SexAI 3230 ACCTGGT A^CC[AT]GGT
SfoI 4906 GGCGCC GGC^GCC
SnaBI 1058 TACGTA TAC^GTA
Sse8387I 3751 CCTGCAGG CCTGCA^GG
StuI 3238 AGGCCT AGG^CCT
Tth111I 3885 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 240 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 2280 GGTACC G^GTACC
2308 GGTACC
AccII 2231 CGCG CG^CG
3763 CGCG
AcyI 4749 GACGTC G[GA]^CG[CT]C
4906 GGCGCC
ApaI 123 GGGCCC GGGCC^C
4654 GGGCCC
Asp700I 417 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1605 GAAACCTTTC
Asp718I 2280 GGTACC G^GTACC
2308 GGTACC
BclI 2660 TGATCA T^GATCA
3158 TGATCA
BglII 705 AGATCT A^GATCT
4057 AGATCT
BsaHI 4749 GACGTC G[GA]^CG[CT]C
4906 GGCGCC
BsmBI 5175 CGTCTC CGTCTC(1/5)
20 GAGACG
Bst1107I 175 GTATAC GTA^TAC
3035 GTATAC
BstUI 2231 CGCG CG^CG
3763 CGCG
BstXI 483 CCAAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2092 CCAGCTCACTGG
BstZ17I 175 GTATAC GTA^TAC
3035 GTATAC
FbaI 2660 TGATCA T^GATCA
3158 TGATCA
HgaI 3761 GACGC GACGC(5/10)
3067 GCGTC
Hin1I 4749 GACGTC G[GA]^CG[CT]C
4906 GGCGCC
KpnI 2280 GGTACC GGTAC^C
2308 GGTACC
MvnI 2231 CGCG CG^CG
3763 CGCG
SmaI 152 CCCGGG CCC^GGG
2157 CCCGGG
SspI 1109 AATATT AAT^ATT
2891 AATATT
XmaCI 152 CCCGGG C^CCGGG
2157 CCCGGG
XmaI 152 CCCGGG C^CCGGG
2157 CCCGGG
XmnI 417 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1605 GAAACCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AseI AsnI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BspMI BsrBI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PshBI PvuII RsaI SacI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII