################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AclI AgeI AhdI Alw44I Aor51HI ApaLI AscI AspEI AviII AvrII BbrPI BcgI BlnI BsaBI BsaI BsiWI Bsp1407I BspDI BsrGI BssHII BstAPI BstBI ClaI CpoI DrdI Eam1105I Eco47III EcoRV FseI FspI HpaI KspI MamI MluI NaeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SfiI SfuI SgrAI SpeI SphI SspBI SwaI XhoI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 4749 GACGTC GACGT^C AccIII 592 TCCGGA T^CCGGA AflII 454 CTTAAG C^TTAAG AspI 3885 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 5199 GGATCC G^GATCC BbeI 4906 GGCGCC GGCGC^C BciVI 5331 GGATAC GTATCC(6/5) BfrI 454 CTTAAG C^TTAAG BlpI 2429 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2429 GCTGAGC GC^T[ACGT]AGC BsaAI 1058 TACGTA [CT]AC^GT[GA] BsaWI 592 TCCGGA [AT]^CCGG[AT] BseAI 592 TCCGGA T^CCGGA BsgI 2324 GTGCAG GTGCAG(16/14) BsiEI 3804 CGGCCG CG[GA][CT]^CG BsmI 3385 GCATTC GAATGC(1/-1) BspEI 592 TCCGGA T^CCGGA BspHI 3796 TCATGA T^CATGA BsrFI 2126 GCCGGT [GA]^CCGG[CT] BssSI 727 CACGAG CACGAG(-5/-1) BstEII 3985 GGTAACC G^GT[ACGT]ACC BstPI 3985 GGTAACC G^GT[ACGT]ACC CelII 2429 GCTGAGC GC^T[ACGT]AGC Cfr10I 2126 GCCGGT [GA]^CCGG[CT] EagI 3804 CGGCCG C^GGCCG EclXI 3804 CGGCCG C^GGCCG Eco52I 3804 CGGCCG C^GGCCG EcoO65I 3985 GGTAACC G^GT[ACGT]ACC EcoRI 5537 GAATTC G^AATTC EcoT22I 1868 ATGCAT ATGCA^T HaeII 4906 GGCGCC [GA]GCGC^[CT] HindIII 3284 AAGCTT A^AGCTT KasI 4906 GGCGCC G^GCGCC MfeI 4975 CAATTG C^AATTG MroI 592 TCCGGA T^CCGGA MunI 4975 CAATTG C^AATTG NarI 4906 GGCGCC GG^CGCC NsiI 1868 ATGCAT ATGCA^T PflFI 3885 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 3752 CTGCAG CTGCA^G RcaI 3796 TCATGA T^CATGA SapI 683 GAAGAGC GCTCTTC(1/4) SbfI 3751 CCTGCAGG CCTGCA^GG SexAI 3230 ACCTGGT A^CC[AT]GGT SfoI 4906 GGCGCC GGC^GCC SnaBI 1058 TACGTA TAC^GTA Sse8387I 3751 CCTGCAGG CCTGCA^GG StuI 3238 AGGCCT AGG^CCT Tth111I 3885 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 240 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### Acc65I 2280 GGTACC G^GTACC 2308 GGTACC AccII 2231 CGCG CG^CG 3763 CGCG AcyI 4749 GACGTC G[GA]^CG[CT]C 4906 GGCGCC ApaI 123 GGGCCC GGGCC^C 4654 GGGCCC Asp700I 417 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1605 GAAACCTTTC Asp718I 2280 GGTACC G^GTACC 2308 GGTACC BclI 2660 TGATCA T^GATCA 3158 TGATCA BglII 705 AGATCT A^GATCT 4057 AGATCT BsaHI 4749 GACGTC G[GA]^CG[CT]C 4906 GGCGCC BsmBI 5175 CGTCTC CGTCTC(1/5) 20 GAGACG Bst1107I 175 GTATAC GTA^TAC 3035 GTATAC BstUI 2231 CGCG CG^CG 3763 CGCG BstXI 483 CCAAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2092 CCAGCTCACTGG BstZ17I 175 GTATAC GTA^TAC 3035 GTATAC FbaI 2660 TGATCA T^GATCA 3158 TGATCA HgaI 3761 GACGC GACGC(5/10) 3067 GCGTC Hin1I 4749 GACGTC G[GA]^CG[CT]C 4906 GGCGCC KpnI 2280 GGTACC GGTAC^C 2308 GGTACC MvnI 2231 CGCG CG^CG 3763 CGCG SmaI 152 CCCGGG CCC^GGG 2157 CCCGGG SspI 1109 AATATT AAT^ATT 2891 AATATT XmaCI 152 CCCGGG C^CCGGG 2157 CCCGGG XmaI 152 CCCGGG C^CCGGG 2157 CCCGGG XmnI 417 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1605 GAAACCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI AseI AsnI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BspMI BsrBI BsrDI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PshBI PvuII RsaI SacI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII