################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AccI AccIII AclI AflII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI AviII BbeI BbrPI BcgI BciVI BclI BfrI BglII BsaAI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BsrGI Bst1107I BstBI BstZ17I ClaI CpoI DraIII Eco47III EcoRV EcoT22I FbaI FspI HindIII HpaI KasI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SalI ScaI SfoI SfuI SgrAI SnaBI SpeI SspBI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AatII 1376 GACGTC GACGT^C AhdI 4508 GACTGGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 4508 GACTGGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3315 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 4671 CCTAGG C^CTAGG BamHI 4675 GGATCC G^GATCC BlnI 4671 CCTAGG C^CTAGG BsaBI 705 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 3035 CGGCCG CG[GA][CT]^CG BsmBI 2236 GAGACG CGTCTC(1/5) BsrDI 3388 GCAATG GCAATG(2/0) BssSI 3575 CACGAG CACGAG(-5/-1) DrdI 1370 GACAAAGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 3035 CGGCCG C^GGCCG Eam1105I 4508 GACTGGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 3035 CGGCCG C^GGCCG Eco52I 3035 CGGCCG C^GGCCG EcoRI 1254 GAATTC G^AATTC FseI 3036 GGCCGGCC GGCCGG^CC HincII 1038 GTCAAC GT[CT]^[GA]AC HindII 1038 GTCAAC GT[CT]^[GA]AC KspI 3032 CCGCGG CCGC^GG MaeII 1377 ACGT A^CGT MamI 705 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC PflFI 3315 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 4553 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 3032 CCGCGG CCGC^GG SbfI 3762 CCTGCAGG CCTGCA^GG SexAI 2210 ACCAGGT A^CC[AT]GGT SmlI 896 CTCAAG C^T[CT][GA]AG SphI 1295 GCATGC GCATG^C Sse8387I 3762 CCTGCAGG CCTGCA^GG SspI 4882 AATATT AAT^ATT Tth111I 3315 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcsI 1254 GAATTC [GA]^AATT[CT] 1767 GAATTT AflIII 2245 ACATGT A^C[GA][CT]GT 4554 ACATGT ApoI 1254 GAATTC [GA]^AATT[CT] 1767 GAATTT Asp700I 577 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1892 GAACCTGTTC BlpI 504 GCTGAGC GC^T[ACGT]AGC 3544 GCTCAGC Bpu1102I 504 GCTGAGC GC^T[ACGT]AGC 3544 GCTCAGC BspLU11I 2245 ACATGT A^CATGT 4554 ACATGT BssHII 175 GCGCGC G^CGCGC 177 GCGCGC BstEII 3320 GGTCACC G^GT[ACGT]ACC 4187 GGTCACC BstPI 3320 GGTCACC G^GT[ACGT]ACC 4187 GGTCACC CelII 504 GCTGAGC GC^T[ACGT]AGC 3544 GCTCAGC DraI 4353 TTTAAA TTT^AAA 4842 TTTAAA EcoO65I 3320 GGTCACC G^GT[ACGT]ACC 4187 GGTCACC NaeI 3037 GCCGGC GCC^GGC 3415 GCCGGC NgoMIV 3037 GCCGGC G^CCGGC 3415 GCCGGC PflMI 976 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2280 CCAGGCCCTGG PstI 1938 CTGCAG CTGCA^G 3763 CTGCAG SfcI 1938 CTGCAG C^T[GA][CT]AG 3763 CTGCAG SfiI 4050 GGCCTGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 4085 GGCCCAGAAGGCC TaqI 1096 TCGA T^CGA 3853 TCGA Tsp509I 1255 AATT ^AATT 1768 AATT TthHB8I 1096 TCGA T^CGA 3853 TCGA Van91I 976 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2280 CCAGGCCCTGG XmnI 577 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1892 GAACCTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### Acc65I AccII AciI AcyI AfaI AluI AlwI AlwNI AocI ApaI Asp718I AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SmaI StuI StyI TfiI Tru9I TseI Tsp45I TspRI XcmI XhoII XmaCI XmaI