##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AviII BbeI BbrPI BcgI
BciVI BclI BfrI BglII BsaAI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BsrGI Bst1107I
BstBI BstZ17I ClaI CpoI DraIII
Eco47III EcoRV EcoT22I FbaI FspI
HindIII HpaI KasI MfeI MluI
MroI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SalI ScaI SfoI SfuI SgrAI
SnaBI SpeI SspBI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 1376 GACGTC GACGT^C
AhdI 4508 GACTGGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 4508 GACTGGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3315 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4671 CCTAGG C^CTAGG
BamHI 4675 GGATCC G^GATCC
BlnI 4671 CCTAGG C^CTAGG
BsaBI 705 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 3035 CGGCCG CG[GA][CT]^CG
BsmBI 2236 GAGACG CGTCTC(1/5)
BsrDI 3388 GCAATG GCAATG(2/0)
BssSI 3575 CACGAG CACGAG(-5/-1)
DrdI 1370 GACAAAGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3035 CGGCCG C^GGCCG
Eam1105I 4508 GACTGGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3035 CGGCCG C^GGCCG
Eco52I 3035 CGGCCG C^GGCCG
EcoRI 1254 GAATTC G^AATTC
FseI 3036 GGCCGGCC GGCCGG^CC
HincII 1038 GTCAAC GT[CT]^[GA]AC
HindII 1038 GTCAAC GT[CT]^[GA]AC
KspI 3032 CCGCGG CCGC^GG
MaeII 1377 ACGT A^CGT
MamI 705 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflFI 3315 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4553 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 3032 CCGCGG CCGC^GG
SbfI 3762 CCTGCAGG CCTGCA^GG
SexAI 2210 ACCAGGT A^CC[AT]GGT
SmlI 896 CTCAAG C^T[CT][GA]AG
SphI 1295 GCATGC GCATG^C
Sse8387I 3762 CCTGCAGG CCTGCA^GG
SspI 4882 AATATT AAT^ATT
Tth111I 3315 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 1254 GAATTC [GA]^AATT[CT]
1767 GAATTT
AflIII 2245 ACATGT A^C[GA][CT]GT
4554 ACATGT
ApoI 1254 GAATTC [GA]^AATT[CT]
1767 GAATTT
Asp700I 577 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1892 GAACCTGTTC
BlpI 504 GCTGAGC GC^T[ACGT]AGC
3544 GCTCAGC
Bpu1102I 504 GCTGAGC GC^T[ACGT]AGC
3544 GCTCAGC
BspLU11I 2245 ACATGT A^CATGT
4554 ACATGT
BssHII 175 GCGCGC G^CGCGC
177 GCGCGC
BstEII 3320 GGTCACC G^GT[ACGT]ACC
4187 GGTCACC
BstPI 3320 GGTCACC G^GT[ACGT]ACC
4187 GGTCACC
CelII 504 GCTGAGC GC^T[ACGT]AGC
3544 GCTCAGC
DraI 4353 TTTAAA TTT^AAA
4842 TTTAAA
EcoO65I 3320 GGTCACC G^GT[ACGT]ACC
4187 GGTCACC
NaeI 3037 GCCGGC GCC^GGC
3415 GCCGGC
NgoMIV 3037 GCCGGC G^CCGGC
3415 GCCGGC
PflMI 976 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2280 CCAGGCCCTGG
PstI 1938 CTGCAG CTGCA^G
3763 CTGCAG
SfcI 1938 CTGCAG C^T[GA][CT]AG
3763 CTGCAG
SfiI 4050 GGCCTGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4085 GGCCCAGAAGGCC
TaqI 1096 TCGA T^CGA
3853 TCGA
Tsp509I 1255 AATT ^AATT
1768 AATT
TthHB8I 1096 TCGA T^CGA
3853 TCGA
Van91I 976 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2280 CCAGGCCCTGG
XmnI 577 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1892 GAACCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
Asp718I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SmaI StuI StyI
TfiI Tru9I TseI Tsp45I TspRI
XcmI XhoII XmaCI XmaI