##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Aor51HI AscI
AseI AsnI Asp718I AspEI BbeI
BbsI BciVI BfrI BpuAI BsaBI
BsaHI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BsrGI BssHII BssSI
BstBI ClaI DraI Eam1105I Eco47III
EcoRI EcoRV EcoT22I HgaI Hin1I
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshBI Psp1406I PvuI SacII SbfI
ScaI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccII 3586 CGCG CG^CG
Asp700I 1928 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 4351 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 2923 GCAGCCTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2145 TGATCA T^GATCA
BglII 563 AGATCT A^GATCT
BlpI 4591 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 4591 GCTAAGC GC^T[ACGT]AGC
BsaWI 3546 TCCGGT [AT]^CCGG[AT]
BsiEI 3627 CGGCCG CG[GA][CT]^CG
BsmI 361 GAATGC GAATGC(1/-1)
BspLU11I 431 ACATGT A^CATGT
BsrFI 3629 GCCGGC [GA]^CCGG[CT]
Bst1107I 2505 GTATAC GTA^TAC
BstUI 3586 CGCG CG^CG
BstZ17I 2505 GTATAC GTA^TAC
CelII 4591 GCTAAGC GC^T[ACGT]AGC
Cfr10I 3629 GCCGGC [GA]^CCGG[CT]
CpoI 3614 CGGACCG CG^G[AT]CCG
DrdI 3210 GACTGCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3627 CGGCCG C^GGCCG
EclXI 3627 CGGCCG C^GGCCG
Eco52I 3627 CGGCCG C^GGCCG
FbaI 2145 TGATCA T^GATCA
FseI 3628 GGCCGGCC GGCCGG^CC
MseI 4373 TTAA T^TAA
MvnI 3586 CGCG CG^CG
NaeI 3629 GCCGGC GCC^GGC
NgoMIV 3629 GCCGGC G^CCGGC
PaeR7I 2230 CTCGAG C^TCGAG
PflFI 4351 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3423 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 3614 CGGACCG CG^G[AT]CCG
SalI 3519 GTCGAC G^TCGAC
SapI 1822 GAAGAGC GCTCTTC(1/4)
SexAI 4246 ACCAGGT A^CC[AT]GGT
SfiI 1357 GGCCTAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 2211 GCATGC GCATG^C
Tru9I 4373 TTAA T^TAA
Tth111I 4351 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2230 CTCGAG C^TCGAG
XmnI 1928 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 2505 GTATAC GT^[AC][GT]AC
3519 GTCGAC
AcsI 467 GAATTT [GA]^AATT[CT]
2634 AAATTC
AflIII 431 ACATGT A^C[GA][CT]GT
2689 ACGTGT
Alw44I 2283 GTGCAC G^TGCAC
2685 GTGCAC
ApaLI 2283 GTGCAC G^TGCAC
2685 GTGCAC
ApoI 467 GAATTT [GA]^AATT[CT]
2634 AAATTC
AviII 149 TGCGCA TGC^GCA
3572 TGCGCA
BamHI 2254 GGATCC G^GATCC
3224 GGATCC
BbrPI 2688 CACGTG CAC^GTG
3898 CACGTG
BspHI 56 TCATGA T^CATGA
252 TCATGA
BsrBI 2198 GAGCGG CCGCTC(-3/-3)
4582 GAGCGG
BstEII 4057 GGTCACC G^GT[ACGT]ACC
4073 GGTCACC
BstPI 4057 GGTCACC G^GT[ACGT]ACC
4073 GGTCACC
EcoO65I 4057 GGTCACC G^GT[ACGT]ACC
4073 GGTCACC
FspI 149 TGCGCA TGC^GCA
3572 TGCGCA
HaeII 1295 AGCGCC [GA]GCGC^[CT]
3923 AGCGCC
HincII 1064 GTCAAC GT[CT]^[GA]AC
3519 GTCGAC
HindII 1064 GTCAAC GT[CT]^[GA]AC
3519 GTCGAC
NcoI 611 CCATGG C^CATGG
3305 CCATGG
PmaCI 2688 CACGTG CAC^GTG
3898 CACGTG
PmlI 2688 CACGTG CAC^GTG
3898 CACGTG
RcaI 56 TCATGA T^CATGA
252 TCATGA
SacI 1084 GAGCTC GAGCT^C
2828 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
AvrII BalI BanI BanII BbvCI
BbvI BcnI BfaI BglI BlnI
BmyI BpmI BsaAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI