##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
Acc65I AccI AccIII AclI AgeI
Alw44I Aor51HI ApaLI AscI Asp700I
Asp718I AspI AviII BbrPI BsaAI
BsaBI BsaWI BseAI BsiWI Bsp1407I
BspDI BspEI BsrGI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DraIII Eco47III EcoRV FseI FspI
KpnI KspI MamI MluI MroI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 4340 GACGTC GACGT^C
AflII 4485 CTTAAG C^TTAAG
AhdI 3376 GACCCATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 5416 CCTGAGG CC^T[ACGT]AGG
AseI 220 ATTAAT AT^TAAT
AsnI 220 ATTAAT AT^TAAT
AspEI 3376 GACCCATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3451 CCTAGG C^CTAGG
BbeI 22 GGCGCC GGCGC^C
BciVI 3544 GTATCC GTATCC(6/5)
BfrI 4485 CTTAAG C^TTAAG
BlnI 3451 CCTAGG C^CTAGG
BsaI 4267 GGTCTC GGTCTC(1/5)
BsiEI 1355 CGGCCG CG[GA][CT]^CG
BspHI 2799 TCATGA T^CATGA
BspLU11I 698 ACATGT A^CATGT
BsrDI 2184 CATTGC GCAATG(2/0)
BssSI 3239 CTCGTG CACGAG(-5/-1)
Bsu36I 5416 CCTGAGG CC^T[ACGT]AGG
EagI 1355 CGGCCG C^GGCCG
Eam1105I 3376 GACCCATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1355 CGGCCG C^GGCCG
Eco52I 1355 CGGCCG C^GGCCG
Eco81I 5416 CCTGAGG CC^T[ACGT]AGG
EcoRI 1369 GAATTC G^AATTC
EcoT22I 3326 ATGCAT ATGCA^T
HindIII 799 AAGCTT A^AGCTT
HpaI 627 GTTAAC GTT^AAC
KasI 22 GGCGCC G^GCGCC
NarI 22 GGCGCC GG^CGCC
NheI 2812 GCTAGC G^CTAGC
NsiI 3326 ATGCAT ATGCA^T
PshBI 220 ATTAAT AT^TAAT
RcaI 2799 TCATGA T^CATGA
SapI 1855 GCTCTTC GCTCTTC(1/4)
ScaI 917 AGTACT AGT^ACT
SexAI 2420 ACCTGGT A^CC[AT]GGT
SfoI 22 GGCGCC GGC^GCC
SmaI 36 CCCGGG CCC^GGG
SspI 4932 AATATT AAT^ATT
XmaCI 36 CCCGGG C^CCGGG
XmaI 36 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 698 ACATGT A^C[GA][CT]GT
5099 ACGTGT
BclI 4195 TGATCA T^GATCA
4977 TGATCA
BsgI 3711 CTGCAC GTGCAG(16/14)
492 GTGCAG
BsmBI 5632 CGTCTC CGTCTC(1/5)
4301 GAGACG
BsrFI 137 GCCGGC [GA]^CCGG[CT]
2917 GCCGGC
BstAPI 1335 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5567 GCAGAGGTTGC
BstEII 1551 GGTGACC G^GT[ACGT]ACC
3174 GGTAACC
BstPI 1551 GGTGACC G^GT[ACGT]ACC
3174 GGTAACC
Cfr10I 137 GCCGGC [GA]^CCGG[CT]
2917 GCCGGC
EcoO65I 1551 GGTGACC G^GT[ACGT]ACC
3174 GGTAACC
FbaI 4195 TGATCA T^GATCA
4977 TGATCA
HaeII 22 GGCGCC [GA]GCGC^[CT]
151 GGCGCT
MfeI 4391 CAATTG C^AATTG
4418 CAATTG
MunI 4391 CAATTG C^AATTG
4418 CAATTG
NaeI 137 GCCGGC GCC^GGC
2917 GCCGGC
NdeI 3209 CATATG CA^TATG
5328 CATATG
NgoMIV 137 GCCGGC G^CCGGC
2917 GCCGGC
XcmI 2150 CCATAACCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2726 CCACTGTCATGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbsI BbvCI BbvI
BcgI BcnI BfaI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XhoII