##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AccI AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
AspEI BbrPI BfrI BglII BsaAI
BsmBI BssHII Bst1107I BstBI BstZ17I
DrdI Eam1105I Eco47III EcoRV EcoT22I
FseI KspI MfeI MluI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
SacII SalI ScaI SfiI SfuI
SgrAI SmaI SnaBI SpeI SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 4357 GGTACC G^GTACC
AocI 3783 CCTGAGG CC^T[ACGT]AGG
Asp718I 4357 GGTACC G^GTACC
AvaI 3100 CTCGGG C^[CT]CG[GA]G
BamHI 3251 GGATCC G^GATCC
BbeI 1296 GGCGCC GGCGC^C
BcgI 853 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4282 TGATCA T^GATCA
BlpI 1432 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1432 GCTCAGC GC^T[ACGT]AGC
BsiWI 3131 CGTACG C^GTACG
BsoBI 3100 CTCGGG C^[CT]CG[GA]G
BspDI 1484 ATCGAT AT^CGAT
BspHI 4226 TCATGA T^CATGA
BsrDI 2417 CATTGC GCAATG(2/0)
BstEII 4170 GGTAACC G^GT[ACGT]ACC
BstPI 4170 GGTAACC G^GT[ACGT]ACC
Bsu36I 3783 CCTGAGG CC^T[ACGT]AGG
CelII 1432 GCTCAGC GC^T[ACGT]AGC
ClaI 1484 ATCGAT AT^CGAT
CpoI 4135 CGGACCG CG^G[AT]CCG
Eco81I 3783 CCTGAGG CC^T[ACGT]AGG
EcoO65I 4170 GGTAACC G^GT[ACGT]ACC
EcoRI 447 GAATTC G^AATTC
FbaI 4282 TGATCA T^GATCA
HpaI 327 GTTAAC GTT^AAC
KasI 1296 GGCGCC G^GCGCC
KpnI 4357 GGTACC GGTAC^C
NaeI 3229 GCCGGC GCC^GGC
NarI 1296 GGCGCC GG^CGCC
NgoMIV 3229 GCCGGC G^CCGGC
RcaI 4226 TCATGA T^CATGA
RsrII 4135 CGGACCG CG^G[AT]CCG
SbfI 5244 CCTGCAGG CCTGCA^GG
SexAI 4545 ACCTGGT A^CC[AT]GGT
SfoI 1296 GGCGCC GGC^GCC
SphI 3225 GCATGC GCATG^C
Sse8387I 5244 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 1296 GGCGCC G[GA]^CG[CT]C
2402 GGCGTC
Alw44I 4075 GTGCAC G^TGCAC
4395 GTGCAC
ApaLI 4075 GTGCAC G^TGCAC
4395 GTGCAC
AviII 72 TGCGCA TGC^GCA
138 TGCGCA
BciVI 3999 GGATAC GTATCC(6/5)
3488 GTATCC
BcnI 100 CCGGG CC^[GC]GG
2407 CCGGG
BglI 3446 GCCCTTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3890 GCCGAATGGGC
BsaBI 1805 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3198 GATGTACATC
BsaHI 1296 GGCGCC G[GA]^CG[CT]C
2402 GGCGTC
Bsp1407I 1234 TGTACA T^GTACA
3200 TGTACA
BspLU11I 1958 ACATGT A^CATGT
5522 ACATGT
BsrBI 4859 CCGCTC CCGCTC(-3/-3)
52 GAGCGG
BsrGI 1234 TGTACA T^GTACA
3200 TGTACA
BstAPI 1502 GCATGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5247 GCAGGCAGTGC
DraI 3558 TTTAAA TTT^AAA
5495 TTTAAA
FspI 72 TGCGCA TGC^GCA
138 TGCGCA
HgaI 2403 GCGTC GACGC(5/10)
2821 GCGTC
Hin1I 1296 GGCGCC G[GA]^CG[CT]C
2402 GGCGTC
HincII 327 GTTAAC GT[CT]^[GA]AC
758 GTTGAC
HindII 327 GTTAAC GT[CT]^[GA]AC
758 GTTGAC
HindIII 4534 AAGCTT A^AGCTT
4927 AAGCTT
MamI 1805 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3198 GATGTACATC
NciI 100 CCGGG CC^[GC]GG
2407 CCGGG
PshAI 2579 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5411 GACCTGTGTC
SacI 1534 GAGCTC GAGCT^C
4578 GAGCTC
SapI 2198 GAAGAGC GCTCTTC(1/4)
64 GCTCTTC
SspBI 1234 TGTACA T^GTACA
3200 TGTACA
SspI 2028 AATATT AAT^ATT
2556 AATATT
XbaI 1277 TCTAGA T^CTAGA
4574 TCTAGA
XcmI 1072 CCACTTTCAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2728 CCAAGATACCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AccIII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI Asp700I AspHI AspI AvaII
AvrII BalI BanI BanII BbsI
BbvCI BbvI BfaI BlnI BmyI
BpmI BpuAI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspEI BspMI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EagI EarI EclXI
Eco52I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MroI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NcoI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XhoII XmnI