##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AccIII AclI AflIII AgeI Aor51HI
ApaI AscI AseI AsnI Asp700I
AvrII BamHI BbrPI BciVI BclI
BglI BlnI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BspLU11I BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI EagI EclXI Eco47III
Eco52I EcoO65I EcoRI FbaI FseI
HpaI KspI MfeI MluI MroI
MunI NheI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SwaI XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 2443 GACGTC GACGT^C
Acc65I 2708 GGTACC G^GTACC
AccII 1260 CGCG CG^CG
AhdI 1528 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 2708 GGTACC G^GTACC
AspEI 1528 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 2686 GGCGCC GGCGC^C
BcgI 2426 GCAAGCTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2589 AGATCT A^GATCT
BsaAI 2576 TACGTG [CT]AC^GT[GA]
BsaBI 348 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 4633 GAATGC GAATGC(1/-1)
BstAPI 1371 GCAGCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1260 CGCG CG^CG
Eam1105I 1528 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 87 CCTGAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 224 ATGCAT ATGCA^T
HincII 1041 GTTGAC GT[CT]^[GA]AC
HindII 1041 GTTGAC GT[CT]^[GA]AC
KasI 2686 GGCGCC G^GCGCC
KpnI 2708 GGTACC GGTAC^C
MamI 348 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1260 CGCG CG^CG
NarI 2686 GGCGCC GG^CGCC
NsiI 224 ATGCAT ATGCA^T
PaeR7I 2453 CTCGAG C^TCGAG
PshAI 2451 GACTCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 2907 CCTGCAGG CCTGCA^GG
SfoI 2686 GGCGCC GGC^GCC
SmaI 2396 CCCGGG CCC^GGG
Sse8387I 2907 CCTGCAGG CCTGCA^GG
StuI 3185 AGGCCT AGG^CCT
XcmI 1607 CCATGCTGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2453 CTCGAG C^TCGAG
XmaCI 2396 CCCGGG C^CCGGG
XmaI 2396 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 3395 GTCTAC GT^[AC][GT]AC
5078 GTAGAC
AflII 3601 CTTAAG C^TTAAG
5172 CTTAAG
AocI 299 CCTCAGG CC^T[ACGT]AGG
4970 CCTGAGG
AspI 145 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
972 GACCAAGTC
AviII 1253 TGCGCA TGC^GCA
5511 TGCGCA
BfrI 3601 CTTAAG C^TTAAG
5172 CTTAAG
Bsp1407I 4239 TGTACA T^GTACA
5540 TGTACA
BspHI 3625 TCATGA T^CATGA
3889 TCATGA
BsrBI 1953 CCGCTC CCGCTC(-3/-3)
1270 GAGCGG
BsrDI 821 GCAATG GCAATG(2/0)
1145 GCAATG
BsrGI 4239 TGTACA T^GTACA
5540 TGTACA
BssSI 502 CACGAG CACGAG(-5/-1)
2302 CACGAG
Bsu36I 299 CCTCAGG CC^T[ACGT]AGG
4970 CCTGAGG
DraIII 3464 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
4454 CACATGGTG
DrdI 142 GACGACAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
486 GACAAATCTGTC
Eco81I 299 CCTCAGG CC^T[ACGT]AGG
4970 CCTGAGG
EcoRV 3037 GATATC GAT^ATC
4540 GATATC
FspI 1253 TGCGCA TGC^GCA
5511 TGCGCA
HgaI 149 GCGTC GACGC(5/10)
4904 GCGTC
MaeII 2444 ACGT A^CGT
2577 ACGT
NaeI 21 GCCGGC GCC^GGC
1847 GCCGGC
NdeI 170 CATATG CA^TATG
4391 CATATG
NgoMIV 21 GCCGGC G^CCGGC
1847 GCCGGC
PflFI 145 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
972 GACCAAGTC
PpuMI 1499 AGGTCCT [GA]G^G[AT]CC[CT]
2366 AGGACCT
RcaI 3625 TCATGA T^CATGA
3889 TCATGA
SspBI 4239 TGTACA T^GTACA
5540 TGTACA
SspI 3917 AATATT AAT^ATT
5150 AATATT
Tth111I 145 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
972 GACCAAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaLI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII