##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AccIII AclI AflIII AgeI Aor51HI
ApaI AscI AseI AsnI Asp700I
AvrII BamHI BbrPI BciVI BclI
BglI BlnI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BspLU11I Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI EagI EclXI Eco47III Eco52I
EcoO65I EcoRI FbaI FseI HpaI
KspI MfeI MluI MroI MunI
NheI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SalI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AatII 2684 GACGTC GACGT^C
Acc65I 2949 GGTACC G^GTACC
AhdI 1769 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 2949 GGTACC G^GTACC
AspEI 1769 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 2667 GCAAGCTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2830 AGATCT A^GATCT
BsaAI 2817 TACGTG [CT]AC^GT[GA]
BsaBI 589 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 4874 GAATGC GAATGC(1/-1)
BssHII 109 GCGCGC G^CGCGC
BstAPI 1612 GCAGCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Eam1105I 1769 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 328 CCTGAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 465 ATGCAT ATGCA^T
HincII 1282 GTTGAC GT[CT]^[GA]AC
HindII 1282 GTTGAC GT[CT]^[GA]AC
KpnI 2949 GGTACC GGTAC^C
MamI 589 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 465 ATGCAT ATGCA^T
PaeR7I 2694 CTCGAG C^TCGAG
PshAI 2692 GACTCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 3148 CCTGCAGG CCTGCA^GG
Sse8387I 3148 CCTGCAGG CCTGCA^GG
StuI 3426 AGGCCT AGG^CCT
XcmI 1848 CCATGCTGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2694 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 3636 GTCTAC GT^[AC][GT]AC
5319 GTAGAC
AflII 3842 CTTAAG C^TTAAG
5413 CTTAAG
AocI 540 CCTCAGG CC^T[ACGT]AGG
5211 CCTGAGG
AspI 386 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1213 GACCAAGTC
AviII 1494 TGCGCA TGC^GCA
5752 TGCGCA
BbeI 143 GGCGCC GGCGC^C
2927 GGCGCC
BfrI 3842 CTTAAG C^TTAAG
5413 CTTAAG
Bsp1407I 4480 TGTACA T^GTACA
5781 TGTACA
BspHI 3866 TCATGA T^CATGA
4130 TCATGA
BsrBI 2194 CCGCTC CCGCTC(-3/-3)
1511 GAGCGG
BsrDI 1062 GCAATG GCAATG(2/0)
1386 GCAATG
BsrGI 4480 TGTACA T^GTACA
5781 TGTACA
BssSI 743 CACGAG CACGAG(-5/-1)
2543 CACGAG
Bsu36I 540 CCTCAGG CC^T[ACGT]AGG
5211 CCTGAGG
DraIII 3705 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
4695 CACATGGTG
Eco81I 540 CCTCAGG CC^T[ACGT]AGG
5211 CCTGAGG
EcoRV 3278 GATATC GAT^ATC
4781 GATATC
FspI 1494 TGCGCA TGC^GCA
5752 TGCGCA
KasI 143 GGCGCC G^GCGCC
2927 GGCGCC
MaeII 2685 ACGT A^CGT
2818 ACGT
NaeI 262 GCCGGC GCC^GGC
2088 GCCGGC
NarI 143 GGCGCC GG^CGCC
2927 GGCGCC
NdeI 411 CATATG CA^TATG
4632 CATATG
NgoMIV 262 GCCGGC G^CCGGC
2088 GCCGGC
PflFI 386 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1213 GACCAAGTC
PpuMI 1740 AGGTCCT [GA]G^G[AT]CC[CT]
2607 AGGACCT
RcaI 3866 TCATGA T^CATGA
4130 TCATGA
SfoI 143 GGCGCC GGC^GCC
2927 GGCGCC
SmaI 139 CCCGGG CCC^GGG
2637 CCCGGG
SspBI 4480 TGTACA T^GTACA
5781 TGTACA
SspI 4158 AATATT AAT^ATT
5391 AATATT
Tth111I 386 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1213 GACCAAGTC
XmaCI 139 CCCGGG C^CCGGG
2637 CCCGGG
XmaI 139 CCCGGG C^CCGGG
2637 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaLI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DrdI DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII