##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AccIII AclI AgeI Aor51HI
ApaI AscI AvaI AviII BbrPI
BbvCI BcgI BglI BglII BlpI
Bpu1102I BsaAI BsaBI BseAI BsiEI
BsiWI BsmBI BsoBI BspEI BspHI
BsrBI BsrFI BssHII BssSI BstBI
CelII Cfr10I CpoI DrdI EagI
EclXI Eco47III Eco52I EcoRV FauI
FseI FspI HgaI HindIII KspI
MamI MfeI MluI MroI MunI
NaeI NgoMIV NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
SfiI SfuI SgrAI SmaI SnaBI
Sse8387I StuI SwaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 2535 GGTACC G^GTACC
AccI 1234 GTATAC GT^[AC][GT]AC
AccII 564 CGCG CG^CG
AhdI 2718 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 873 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 2535 GGTACC G^GTACC
AspEI 2718 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1695 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1913 TGGCCA TGG^CCA
BamHI 634 GGATCC G^GATCC
BciVI 11 GGATAC GTATCC(6/5)
BcnI 1339 CCCGG CC^[GC]GG
BsaI 3914 GGTCTC GGTCTC(1/5)
BsaWI 3191 ACCGGA [AT]^CCGG[AT]
BspDI 4058 ATCGAT AT^CGAT
Bst1107I 1234 GTATAC GTA^TAC
BstEII 3360 GGTGACC G^GT[ACGT]ACC
BstPI 3360 GGTGACC G^GT[ACGT]ACC
BstUI 564 CGCG CG^CG
BstXI 2928 CCAGAACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1234 GTATAC GTA^TAC
ClaI 4058 ATCGAT AT^CGAT
Eam1105I 2718 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 4390 CCTTCTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3360 GGTGACC G^GT[ACGT]ACC
EcoRI 2913 GAATTC G^AATTC
EcoT22I 994 ATGCAT ATGCA^T
KpnI 2535 GGTACC GGTAC^C
MluNI 1913 TGGCCA TGG^CCA
MscI 1913 TGGCCA TGG^CCA
MvnI 564 CGCG CG^CG
NciI 1339 CCCGG CC^[GC]GG
NheI 2384 GCTAGC G^CTAGC
NsiI 994 ATGCAT ATGCA^T
PflFI 1695 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 638 CCATTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 3024 GACTC GAGTC(4/5)
PpuMI 2593 GGGACCT [GA]G^G[AT]CC[CT]
PstI 3127 CTGCAG CTGCA^G
PvuII 4754 CAGCTG CAG^CTG
ScaI 441 AGTACT AGT^ACT
SexAI 3572 ACCTGGT A^CC[AT]GGT
SphI 2923 GCATGC GCATG^C
Tth111I 1695 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 638 CCATTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1720 TCTAGA T^CTAGA
XcmI 4547 CCAATACTTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 2542 GGCGCC G[GA]^CG[CT]C
2576 GGCGCC
AflII 598 CTTAAG C^TTAAG
3011 CTTAAG
Alw44I 683 GTGCAC G^TGCAC
1206 GTGCAC
ApaLI 683 GTGCAC G^TGCAC
1206 GTGCAC
Asp700I 2420 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4385 GAACCCCTTC
AvrII 2264 CCTAGG C^CTAGG
4670 CCTAGG
BbeI 2542 GGCGCC GGCGC^C
2576 GGCGCC
BfrI 598 CTTAAG C^TTAAG
3011 CTTAAG
BlnI 2264 CCTAGG C^CTAGG
4670 CCTAGG
BsaHI 2542 GGCGCC G[GA]^CG[CT]C
2576 GGCGCC
BsmI 3104 GAATGC GAATGC(1/-1)
1141 GCATTC
Cac8I 2384 GCTAGC GC[ACGT]^[ACGT]GC
2923 GCATGC
CfoI 2543 GCGC GCG^C
2577 GCGC
DraIII 59 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
1209 CACAACGTG
DsaI 643 CCATGG C^C[GA][CT]GG
1526 CCATGG
EaeI 1913 TGGCCA [CT]^GGCC[GA]
2844 CGGCCA
HaeII 2542 GGCGCC [GA]GCGC^[CT]
2576 GGCGCC
HapII 1340 CCGG C^CGG
3192 CCGG
HhaI 2543 GCGC GCG^C
2577 GCGC
Hin1I 2542 GGCGCC G[GA]^CG[CT]C
2576 GGCGCC
HinP1I 2543 GCGC G^CGC
2577 GCGC
HincII 3553 GTTAAC GT[CT]^[GA]AC
4196 GTTAAC
HindII 3553 GTTAAC GT[CT]^[GA]AC
4196 GTTAAC
HpaI 3553 GTTAAC GTT^AAC
4196 GTTAAC
HpaII 1340 CCGG C^CGG
3192 CCGG
KasI 2542 GGCGCC G^GCGCC
2576 GGCGCC
MspA1I 3671 CCGCTG C[AC]G^C[GT]G
4754 CAGCTG
MspI 1340 CCGG C^CGG
3192 CCGG
NarI 2542 GGCGCC GG^CGCC
2576 GGCGCC
NcoI 643 CCATGG C^CATGG
1526 CCATGG
SfaNI 2898 GATGC GCATC(5/9)
4113 GCATC
SfcI 2949 CTACAG C^T[GA][CT]AG
3127 CTGCAG
SfoI 2542 GGCGCC GGC^GCC
2576 GGCGCC
XmnI 2420 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4385 GAACCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AocI ApoI AseI AsnI
AspHI AvaII BanI BanII BbsI
BbvI BclI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspLU11I BspMI BsrDI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Bsu36I Cfr13I DdeI DpnII
DraI DraII EarI Eco81I EcoO109I
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeI NdeII NlaIII NlaIV
NspI PshBI RsaI SacI Sau3AI
Sau96I ScrFI SmlI SpeI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII