##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AspEI AviII
BamHI BcgI BglI BglII BlpI
Bpu1102I BseAI BsiEI BsiWI BsmBI
BspDI BspEI BssHII CelII ClaI
CpoI DraIII DsaI EagI Eam1105I
EclXI Eco47III Eco52I FseI FspI
HindIII KspI MluI MroI NaeI
NcoI NgoMIV NheI NotI NruI
PacI PaeR7I PinAI PmeI PshAI
PvuI RsrII SacI SacII SalI
SbfI SfiI SgrAI SnaBI Sse8387I
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 68 GGTACC G^GTACC
AclI 4191 AACGTT AA^CGTT
AcyI 2442 GGCGCC G[GA]^CG[CT]C
AseI 3930 ATTAAT AT^TAAT
AsnI 3930 ATTAAT AT^TAAT
Asp718I 68 GGTACC G^GTACC
AvrII 5693 CCTAGG C^CTAGG
BbeI 2442 GGCGCC GGCGC^C
BbrPI 2658 CACGTG CAC^GTG
BciVI 1897 GGATAC GTATCC(6/5)
BlnI 5693 CCTAGG C^CTAGG
BsaAI 2658 CACGTG [CT]AC^GT[GA]
BsaHI 2442 GGCGCC G[GA]^CG[CT]C
BsaWI 904 ACCGGA [AT]^CCGG[AT]
BsrBI 31 GAGCGG CCGCTC(-3/-3)
BsrFI 882 GCCGGT [GA]^CCGG[CT]
BssSI 2532 CTCGTG CACGAG(-5/-1)
BstAPI 2377 GCAAGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 5636 TTCGAA TT^CGAA
BstEII 2773 GGTGACC G^GT[ACGT]ACC
BstPI 2773 GGTGACC G^GT[ACGT]ACC
Cfr10I 882 GCCGGT [GA]^CCGG[CT]
DrdI 76 GACGGGTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 5693 CCTAGGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2773 GGTGACC G^GT[ACGT]ACC
EcoT22I 1807 ATGCAT ATGCA^T
HaeII 2442 GGCGCC [GA]GCGC^[CT]
HgaI 100 GCGTC GACGC(5/10)
Hin1I 2442 GGCGCC G[GA]^CG[CT]C
KasI 2442 GGCGCC G^GCGCC
KpnI 68 GGTACC GGTAC^C
NarI 2442 GGCGCC GG^CGCC
NdeI 2842 CATATG CA^TATG
NsiI 1807 ATGCAT ATGCA^T
NspV 5636 TTCGAA TT^CGAA
PmaCI 2658 CACGTG CAC^GTG
PmlI 2658 CACGTG CAC^GTG
PshBI 3930 ATTAAT AT^TAAT
Psp1406I 4191 AACGTT AA^CGTT
SapI 4700 GAAGAGC GCTCTTC(1/4)
SfoI 2442 GGCGCC GGC^GCC
SfuI 5636 TTCGAA TT^CGAA
SpeI 4865 ACTAGT A^CTAGT
SphI 3503 GCATGC GCATG^C
StuI 4501 AGGCCT AGG^CCT
XbaI 4986 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflII 3599 CTTAAG C^TTAAG
4829 CTTAAG
AflIII 1725 ACATGT A^C[GA][CT]GT
5845 ACATGT
AlwNI 1233 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
1266 CAGAATCTG
AocI 108 CCTGAGG CC^T[ACGT]AGG
3214 CCTCAGG
ApaI 4320 GGGCCC GGGCC^C
4488 GGGCCC
AspHI 439 GTGCTC G[AT]GC[AT]^C
1964 GAGCAC
AspI 2348 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4760 GACTATGTC
BclI 5376 TGATCA T^GATCA
5612 TGATCA
BfrI 3599 CTTAAG C^TTAAG
4829 CTTAAG
BsaBI 2160 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5332 GATGAATATC
BsaI 2229 GAGACC GGTCTC(1/5)
2515 GGTCTC
BseRI 57 GAGGAG GAGGAG(10/8)
2816 GAGGAG
BsgI 2739 CTGCAC GTGCAG(16/14)
1225 GTGCAG
BsiHKAI 439 GTGCTC G[AT]GC[AT]^C
1964 GAGCAC
BspLU11I 1725 ACATGT A^CATGT
5845 ACATGT
Bst1107I 1131 GTATAC GTA^TAC
4622 GTATAC
BstXI 995 CCATTTGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2423 CCAAGTAGCTGG
BstZ17I 1131 GTATAC GTA^TAC
4622 GTATAC
Bsu36I 108 CCTGAGG CC^T[ACGT]AGG
3214 CCTCAGG
Eco81I 108 CCTGAGG CC^T[ACGT]AGG
3214 CCTCAGG
FbaI 5376 TGATCA T^GATCA
5612 TGATCA
HincII 3817 GTTAAC GT[CT]^[GA]AC
4215 GTTAAC
HindII 3817 GTTAAC GT[CT]^[GA]AC
4215 GTTAAC
HpaI 3817 GTTAAC GTT^AAC
4215 GTTAAC
MamI 2160 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5332 GATGAATATC
PflFI 2348 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4760 GACTATGTC
PpuMI 1234 AGGACCT [GA]G^G[AT]CC[CT]
1319 AGGTCCT
Tth111I 2348 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4760 GACTATGTC
XcmI 2841 CCATATGCCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2849 CCAGTGGATTCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI ApoI Asp700I AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspHI BspMI BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EaeI EarI
EcoO109I EcoRI EcoRII EcoRV EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MunI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScaI ScrFI
SexAI SfaNI SfcI SmaI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmaCI XmaI
XmnI