################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AatII AccIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AspEI AviII BamHI BcgI BglI BglII BlpI Bpu1102I BseAI BsiEI BsiWI BsmBI BspDI BspEI BssHII CelII ClaI CpoI DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I FseI FspI HindIII KspI MluI MroI NaeI NcoI NgoMIV NheI NotI NruI PacI PaeR7I PinAI PmeI PshAI PvuI RsrII SacI SacII SalI SbfI SfiI SgrAI SnaBI Sse8387I SwaI XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 68 GGTACC G^GTACC AclI 4191 AACGTT AA^CGTT AcyI 2442 GGCGCC G[GA]^CG[CT]C AseI 3930 ATTAAT AT^TAAT AsnI 3930 ATTAAT AT^TAAT Asp718I 68 GGTACC G^GTACC AvrII 5693 CCTAGG C^CTAGG BbeI 2442 GGCGCC GGCGC^C BbrPI 2658 CACGTG CAC^GTG BciVI 1897 GGATAC GTATCC(6/5) BlnI 5693 CCTAGG C^CTAGG BsaAI 2658 CACGTG [CT]AC^GT[GA] BsaHI 2442 GGCGCC G[GA]^CG[CT]C BsaWI 904 ACCGGA [AT]^CCGG[AT] BsrBI 31 GAGCGG CCGCTC(-3/-3) BsrFI 882 GCCGGT [GA]^CCGG[CT] BssSI 2532 CTCGTG CACGAG(-5/-1) BstAPI 2377 GCAAGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 5636 TTCGAA TT^CGAA BstEII 2773 GGTGACC G^GT[ACGT]ACC BstPI 2773 GGTGACC G^GT[ACGT]ACC Cfr10I 882 GCCGGT [GA]^CCGG[CT] DrdI 76 GACGGGTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 5693 CCTAGGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2773 GGTGACC G^GT[ACGT]ACC EcoT22I 1807 ATGCAT ATGCA^T HaeII 2442 GGCGCC [GA]GCGC^[CT] HgaI 100 GCGTC GACGC(5/10) Hin1I 2442 GGCGCC G[GA]^CG[CT]C KasI 2442 GGCGCC G^GCGCC KpnI 68 GGTACC GGTAC^C NarI 2442 GGCGCC GG^CGCC NdeI 2842 CATATG CA^TATG NsiI 1807 ATGCAT ATGCA^T NspV 5636 TTCGAA TT^CGAA PmaCI 2658 CACGTG CAC^GTG PmlI 2658 CACGTG CAC^GTG PshBI 3930 ATTAAT AT^TAAT Psp1406I 4191 AACGTT AA^CGTT SapI 4700 GAAGAGC GCTCTTC(1/4) SfoI 2442 GGCGCC GGC^GCC SfuI 5636 TTCGAA TT^CGAA SpeI 4865 ACTAGT A^CTAGT SphI 3503 GCATGC GCATG^C StuI 4501 AGGCCT AGG^CCT XbaI 4986 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AflII 3599 CTTAAG C^TTAAG 4829 CTTAAG AflIII 1725 ACATGT A^C[GA][CT]GT 5845 ACATGT AlwNI 1233 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG 1266 CAGAATCTG AocI 108 CCTGAGG CC^T[ACGT]AGG 3214 CCTCAGG ApaI 4320 GGGCCC GGGCC^C 4488 GGGCCC AspHI 439 GTGCTC G[AT]GC[AT]^C 1964 GAGCAC AspI 2348 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4760 GACTATGTC BclI 5376 TGATCA T^GATCA 5612 TGATCA BfrI 3599 CTTAAG C^TTAAG 4829 CTTAAG BsaBI 2160 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5332 GATGAATATC BsaI 2229 GAGACC GGTCTC(1/5) 2515 GGTCTC BseRI 57 GAGGAG GAGGAG(10/8) 2816 GAGGAG BsgI 2739 CTGCAC GTGCAG(16/14) 1225 GTGCAG BsiHKAI 439 GTGCTC G[AT]GC[AT]^C 1964 GAGCAC BspLU11I 1725 ACATGT A^CATGT 5845 ACATGT Bst1107I 1131 GTATAC GTA^TAC 4622 GTATAC BstXI 995 CCATTTGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2423 CCAAGTAGCTGG BstZ17I 1131 GTATAC GTA^TAC 4622 GTATAC Bsu36I 108 CCTGAGG CC^T[ACGT]AGG 3214 CCTCAGG Eco81I 108 CCTGAGG CC^T[ACGT]AGG 3214 CCTCAGG FbaI 5376 TGATCA T^GATCA 5612 TGATCA HincII 3817 GTTAAC GT[CT]^[GA]AC 4215 GTTAAC HindII 3817 GTTAAC GT[CT]^[GA]AC 4215 GTTAAC HpaI 3817 GTTAAC GTT^AAC 4215 GTTAAC MamI 2160 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5332 GATGAATATC PflFI 2348 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4760 GACTATGTC PpuMI 1234 AGGACCT [GA]G^G[AT]CC[CT] 1319 AGGTCCT Tth111I 2348 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4760 GACTATGTC XcmI 2841 CCATATGCCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2849 CCAGTGGATTCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwI ApoI Asp700I AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspHI BspMI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRI EcoRII EcoRV EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MflI MluNI MnlI MscI MseI MslI MspA1I MspI MunI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RcaI RsaI Sau3AI Sau96I ScaI ScrFI SexAI SfaNI SfcI SmaI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI XmnI