################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## Acc65I AccII AccIII AclI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AviII AvrII BanI BbeI BbrPI BclI BlnI BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstPI BstUI Bsu36I Cfr10I ClaI CpoI DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacI SacII SalI SbfI SfiI SfoI SgrAI SmaI SnaBI Sse8387I SspBI SwaI Van91I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AatII 201 GACGTC GACGT^C AcyI 201 GACGTC G[GA]^CG[CT]C AvaI 4660 CTCGAG C^[CT]CG[GA]G BamHI 4468 GGATCC G^GATCC BanII 276 GGGCTC G[GA]GC[CT]^C BcgI 761 GCACTATGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 2245 GCCTCTTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 4848 AGATCT A^GATCT BlpI 534 GCTCAGC GC^T[ACGT]AGC Bpu1102I 534 GCTCAGC GC^T[ACGT]AGC BsaHI 201 GACGTC G[GA]^CG[CT]C BsmI 4479 GAATGC GAATGC(1/-1) BsoBI 4660 CTCGAG C^[CT]CG[GA]G BstBI 874 TTCGAA TT^CGAA BstXI 3269 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 534 GCTCAGC GC^T[ACGT]AGC CfoI 3071 GCGC GCG^C DrdI 1209 GACCAAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HhaI 3071 GCGC GCG^C Hin1I 201 GACGTC G[GA]^CG[CT]C HinP1I 3071 GCGC G^CGC HindIII 4609 AAGCTT A^AGCTT MaeII 202 ACGT A^CGT MspA1I 126 CAGCTG C[AC]G^C[GT]G NdeI 1047 CATATG CA^TATG NspV 874 TTCGAA TT^CGAA PaeR7I 4660 CTCGAG C^TCGAG PvuII 126 CAGCTG CAG^CTG SexAI 419 ACCTGGT A^CC[AT]GGT SfuI 874 TTCGAA TT^CGAA SpeI 4100 ACTAGT A^CTAGT SphI 3155 GCATGC GCATG^C StuI 4785 AGGCCT AGG^CCT XbaI 1320 TCTAGA T^CTAGA XcmI 2718 CCATTACTAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 4660 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AflII 3606 CTTAAG C^TTAAG 4490 CTTAAG AspI 155 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3032 GACAGAGTC BalI 3216 TGGCCA TGG^CCA 3645 TGGCCA BbvCI 2865 CCTCAGC CCTCAGC(-5/-2) 3125 CCTCAGC BcnI 1082 CCGGG CC^[GC]GG 1431 CCGGG BfrI 3606 CTTAAG C^TTAAG 4490 CTTAAG BmyI 276 GGGCTC G[AGT]GC[ACT]^C 785 GTGCCC Bsp1286I 276 GGGCTC G[AGT]GC[ACT]^C 785 GTGCCC BspHI 1682 TCATGA T^CATGA 2121 TCATGA Bst1107I 171 GTATAC GTA^TAC 3980 GTATAC BstZ17I 171 GTATAC GTA^TAC 3980 GTATAC EaeI 3216 TGGCCA [CT]^GGCC[GA] 3645 TGGCCA EcoRI 1176 GAATTC G^AATTC 5296 GAATTC FauI 1 CCCGC CCCGC(4/6) 251 GCGGG HapII 1082 CCGG C^CGG 1431 CCGG HpaII 1082 CCGG C^CGG 1431 CCGG MluNI 3216 TGGCCA TGG^CCA 3645 TGGCCA MscI 3216 TGGCCA TGG^CCA 3645 TGGCCA MspI 1082 CCGG C^CGG 1431 CCGG NciI 1082 CCGGG CC^[GC]GG 1431 CCGGG NlaIV 3307 GGCCCC GG[ACGT]^[ACGT]CC 4468 GGATCC PflFI 155 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3032 GACAGAGTC PpuMI 1535 AGGACCT [GA]G^G[AT]CC[CT] 3582 AGGACCT PstI 4538 CTGCAG CTGCA^G 5454 CTGCAG RcaI 1682 TCATGA T^CATGA 2121 TCATGA SapI 1917 GCTCTTC GCTCTTC(1/4) 3947 GCTCTTC ScaI 2313 AGTACT AGT^ACT 3456 AGTACT Tth111I 155 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3032 GACAGAGTC ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AseI AsnI Asp700I AvaII BbsI BbvI BciVI BfaI BpmI BpuAI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI BspLU11I BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NspI PleI PshBI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI