##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspHI
AviII AvrII BanI BbeI BbrPI
BclI BlnI BsaAI BsaBI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI Bsp1407I BspDI BspEI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI
BstEII BstPI BstUI Bsu36I Cfr10I
ClaI CpoI DraIII DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI PacI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SfiI SfoI SgrAI SmaI
SnaBI Sse8387I SspBI SwaI Van91I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 201 GACGTC GACGT^C
AcyI 201 GACGTC G[GA]^CG[CT]C
AvaI 4660 CTCGAG C^[CT]CG[GA]G
BamHI 4468 GGATCC G^GATCC
BanII 276 GGGCTC G[GA]GC[CT]^C
BcgI 761 GCACTATGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 2245 GCCTCTTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4848 AGATCT A^GATCT
BlpI 534 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 534 GCTCAGC GC^T[ACGT]AGC
BsaHI 201 GACGTC G[GA]^CG[CT]C
BsmI 4479 GAATGC GAATGC(1/-1)
BsoBI 4660 CTCGAG C^[CT]CG[GA]G
BstBI 874 TTCGAA TT^CGAA
BstXI 3269 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 534 GCTCAGC GC^T[ACGT]AGC
CfoI 3071 GCGC GCG^C
DrdI 1209 GACCAAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HhaI 3071 GCGC GCG^C
Hin1I 201 GACGTC G[GA]^CG[CT]C
HinP1I 3071 GCGC G^CGC
HindIII 4609 AAGCTT A^AGCTT
MaeII 202 ACGT A^CGT
MspA1I 126 CAGCTG C[AC]G^C[GT]G
NdeI 1047 CATATG CA^TATG
NspV 874 TTCGAA TT^CGAA
PaeR7I 4660 CTCGAG C^TCGAG
PvuII 126 CAGCTG CAG^CTG
SexAI 419 ACCTGGT A^CC[AT]GGT
SfuI 874 TTCGAA TT^CGAA
SpeI 4100 ACTAGT A^CTAGT
SphI 3155 GCATGC GCATG^C
StuI 4785 AGGCCT AGG^CCT
XbaI 1320 TCTAGA T^CTAGA
XcmI 2718 CCATTACTAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4660 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflII 3606 CTTAAG C^TTAAG
4490 CTTAAG
AspI 155 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3032 GACAGAGTC
BalI 3216 TGGCCA TGG^CCA
3645 TGGCCA
BbvCI 2865 CCTCAGC CCTCAGC(-5/-2)
3125 CCTCAGC
BcnI 1082 CCGGG CC^[GC]GG
1431 CCGGG
BfrI 3606 CTTAAG C^TTAAG
4490 CTTAAG
BmyI 276 GGGCTC G[AGT]GC[ACT]^C
785 GTGCCC
Bsp1286I 276 GGGCTC G[AGT]GC[ACT]^C
785 GTGCCC
BspHI 1682 TCATGA T^CATGA
2121 TCATGA
Bst1107I 171 GTATAC GTA^TAC
3980 GTATAC
BstZ17I 171 GTATAC GTA^TAC
3980 GTATAC
EaeI 3216 TGGCCA [CT]^GGCC[GA]
3645 TGGCCA
EcoRI 1176 GAATTC G^AATTC
5296 GAATTC
FauI 1 CCCGC CCCGC(4/6)
251 GCGGG
HapII 1082 CCGG C^CGG
1431 CCGG
HpaII 1082 CCGG C^CGG
1431 CCGG
MluNI 3216 TGGCCA TGG^CCA
3645 TGGCCA
MscI 3216 TGGCCA TGG^CCA
3645 TGGCCA
MspI 1082 CCGG C^CGG
1431 CCGG
NciI 1082 CCGGG CC^[GC]GG
1431 CCGGG
NlaIV 3307 GGCCCC GG[ACGT]^[ACGT]CC
4468 GGATCC
PflFI 155 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3032 GACAGAGTC
PpuMI 1535 AGGACCT [GA]G^G[AT]CC[CT]
3582 AGGACCT
PstI 4538 CTGCAG CTGCA^G
5454 CTGCAG
RcaI 1682 TCATGA T^CATGA
2121 TCATGA
SapI 1917 GCTCTTC GCTCTTC(1/4)
3947 GCTCTTC
ScaI 2313 AGTACT AGT^ACT
3456 AGTACT
Tth111I 155 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3032 GACAGAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AvaII BbsI BbvI
BciVI BfaI BpmI BpuAI BsaI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BspLU11I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NspI PleI PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI