##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccI AclI AflII AgeI
Alw44I ApaLI AscI AseI AsnI
Asp700I BciVI BclI BfrI BsaBI
BsiWI Bsp1407I BspDI BsrGI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
DraIII DrdI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
KspI MamI MfeI MluI MunI
NdeI NheI NruI NsiI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI SacII SalI SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XmnI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 3193 AAATTT [GA]^AATT[CT]
AhdI 5963 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 3193 AAATTT [GA]^AATT[CT]
AspEI 5963 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2153 TGCGCA TGC^GCA
BamHI 647 GGATCC G^GATCC
BbsI 3372 GAAGAC GAAGAC(2/6)
BcgI 4718 CGATTCTCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 2885 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2885 GCTTAGC GC^T[ACGT]AGC
BpuAI 3372 GAAGAC GAAGAC(2/6)
BspHI 1037 TCATGA T^CATGA
BspLU11I 749 ACATGT A^CATGT
BssSI 5216 CTCGTG CACGAG(-5/-1)
CelII 2885 GCTTAGC GC^T[ACGT]AGC
CpoI 437 CGGTCCG CG^G[AT]CCG
Eam1105I 5963 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 2153 TGCGCA TGC^GCA
HincII 730 GTCAAC GT[CT]^[GA]AC
HindII 730 GTCAAC GT[CT]^[GA]AC
NotI 98 GCGGCCGC GC^GGCCGC
PaeR7I 3251 CTCGAG C^TCGAG
RcaI 1037 TCATGA T^CATGA
RsrII 437 CGGTCCG CG^G[AT]CCG
ScaI 875 AGTACT AGT^ACT
XbaI 801 TCTAGA T^CTAGA
XhoI 3251 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 2372 GGTACC G^GTACC
4543 GGTACC
Asp718I 2372 GGTACC G^GTACC
4543 GGTACC
AvrII 5023 CCTAGG C^CTAGG
5998 CCTAGG
BbrPI 321 CACGTG CAC^GTG
1086 CACGTG
BglII 863 AGATCT A^GATCT
1658 AGATCT
BlnI 5023 CCTAGG C^CTAGG
5998 CCTAGG
BsmBI 5857 CGTCTC CGTCTC(1/5)
4800 GAGACG
BsmI 2115 GAATGC GAATGC(1/-1)
3287 GCATTC
BsrDI 4424 CATTGC GCAATG(2/0)
3074 GCAATG
DraI 3190 TTTAAA TTT^AAA
3398 TTTAAA
EagI 99 CGGCCG C^GGCCG
847 CGGCCG
EclXI 99 CGGCCG C^GGCCG
847 CGGCCG
Eco52I 99 CGGCCG C^GGCCG
847 CGGCCG
KpnI 2372 GGTACC GGTAC^C
4543 GGTACC
NaeI 4021 GCCGGC GCC^GGC
5771 GCCGGC
NgoMIV 4021 GCCGGC G^CCGGC
5771 GCCGGC
NspI 749 ACATGT [GA]CATG^[CT]
1445 ACATGC
PmaCI 321 CACGTG CAC^GTG
1086 CACGTG
PmlI 321 CACGTG CAC^GTG
1086 CACGTG
PshAI 3738 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4047 GACATTAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 145 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
Aor51HI ApaI AspHI AspI AvaI
AvaII BalI BanI BanII BbeI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaAI BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspEI BspMI
BsrBI BsrFI BsrI BssHII BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco47III Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV PflFI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmaCI XmaI