##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AccIII AclI AflII AgeI AseI
AsnI Asp700I AvrII BcgI BfrI
BlnI BsaWI BseAI BsiWI BspDI
BspEI BspLU11I Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
EcoO65I EcoRI EcoRV EcoT22I FseI
HpaI MfeI MluI MroI MunI
NdeI NheI NotI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SbfI
ScaI SexAI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AflIII 3915 ACGTGT A^C[GA][CT]GT
AhdI 2104 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1601 GTGCAC G^TGCAC
AocI 4631 CCTTAGG CC^T[ACGT]AGG
Aor51HI 1996 AGCGCT AGC^GCT
ApaLI 1601 GTGCAC G^TGCAC
AscI 3118 GGCGCGCC GG^CGCGCC
AspEI 2104 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4596 TGCGCA TGC^GCA
BamHI 4416 GGATCC G^GATCC
BbrPI 4202 CACGTG CAC^GTG
BbvCI 2809 GCTGAGG CCTCAGC(-5/-2)
BciVI 412 GGATAC GTATCC(6/5)
BfaI 5054 CTAG C^TAG
BglII 3653 AGATCT A^GATCT
BlpI 3288 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3288 GCTCAGC GC^T[ACGT]AGC
BsmBI 3384 CGTCTC CGTCTC(1/5)
BssHII 3119 GCGCGC G^CGCGC
BstAPI 4576 GCATCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 4631 CCTTAGG CC^T[ACGT]AGG
CelII 3288 GCTCAGC GC^T[ACGT]AGC
Eam1105I 2104 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1996 AGCGCT AGC^GCT
Eco81I 4631 CCTTAGG CC^T[ACGT]AGG
FspI 4596 TGCGCA TGC^GCA
HincII 3778 GTCGAC GT[CT]^[GA]AC
HindII 3778 GTCGAC GT[CT]^[GA]AC
HindIII 1957 AAGCTT A^AGCTT
MaeI 5054 CTAG C^TAG
NaeI 107 GCCGGC GCC^GGC
NcoI 2711 CCATGG C^CATGG
NgoMIV 107 GCCGGC G^CCGGC
NruI 5059 TCGCGA TCG^CGA
PmaCI 4202 CACGTG CAC^GTG
PmlI 4202 CACGTG CAC^GTG
SalI 3778 GTCGAC G^TCGAC
SfiI 2028 GGCCCCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1085 GCATGC GCATG^C
XbaI 5053 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AatII 526 GACGTC GACGT^C
894 GACGTC
Acc65I 1372 GGTACC G^GTACC
2282 GGTACC
AcsI 3174 AAATTC [GA]^AATT[CT]
4934 GAATTT
ApoI 3174 AAATTC [GA]^AATT[CT]
4934 GAATTT
Asp718I 1372 GGTACC G^GTACC
2282 GGTACC
BbeI 160 GGCGCC GGCGC^C
2207 GGCGCC
BclI 710 TGATCA T^GATCA
3536 TGATCA
BsaBI 1134 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1326 GATGGAGATC
BsaI 641 GAGACC GGTCTC(1/5)
2680 GAGACC
BsmI 2556 GAATGC GAATGC(1/-1)
3620 GCATTC
Bsp1407I 716 TGTACA T^GTACA
3710 TGTACA
BspHI 659 TCATGA T^CATGA
4989 TCATGA
BsrDI 3462 CATTGC GCAATG(2/0)
1183 GCAATG
BsrGI 716 TGTACA T^GTACA
3710 TGTACA
BstXI 638 CCAGAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3242 CCATCGTGCTGG
DraIII 4199 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
4213 CACTTGGTG
FbaI 710 TGATCA T^GATCA
3536 TGATCA
KasI 160 GGCGCC G^GCGCC
2207 GGCGCC
KpnI 1372 GGTACC GGTAC^C
2282 GGTACC
MamI 1134 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1326 GATGGAGATC
NarI 160 GGCGCC GG^CGCC
2207 GGCGCC
RcaI 659 TCATGA T^CATGA
4989 TCATGA
SapI 2194 GAAGAGC GCTCTTC(1/4)
2724 GAAGAGC
SfoI 160 GGCGCC GGC^GCC
2207 GGCGCC
SspBI 716 TGTACA T^GTACA
3710 TGTACA
StuI 2064 AGGCCT AGG^CCT
4510 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AspI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BglI
BmyI BpmI BpuAI BsaAI BsaHI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EagI EarI EclXI Eco52I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I KspI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SacI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmaCI
XmaI