##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
AscI Asp700I AvrII BbeI BcgI
BfrI BglII BlnI BsaWI BseAI
BsmI Bsp1407I BspEI BsrGI BssHII
Bst1107I BstBI BstZ17I CpoI EcoRV
HindIII HpaI KasI KspI MluI
MroI NarI NdeI NotI NruI
NspV PacI PaeR7I PinAI PmeI
Psp1406I RsrII SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SpeI Sse8387I SspBI SwaI XcmI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AcsI 913 GAATTC [GA]^AATT[CT]
AhdI 2739 GACACAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3234 GTGCAC G^TGCAC
AocI 3717 CCTCAGG CC^T[ACGT]AGG
Aor51HI 3594 AGCGCT AGC^GCT
ApaLI 3234 GTGCAC G^TGCAC
ApoI 913 GAATTC [GA]^AATT[CT]
AseI 1386 ATTAAT AT^TAAT
AsnI 1386 ATTAAT AT^TAAT
AspEI 2739 GACACAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3425 TGCGCA TGC^GCA
BamHI 2969 GGATCC G^GATCC
BciVI 837 GTATCC GTATCC(6/5)
BclI 1004 TGATCA T^GATCA
BsaBI 1591 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 1691 CGTACG C^GTACG
BsmBI 4663 GAGACG CGTCTC(1/5)
BspDI 2681 ATCGAT AT^CGAT
BsrDI 351 GCAATG GCAATG(2/0)
BstAPI 2544 GCACTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 3717 CCTCAGG CC^T[ACGT]AGG
ClaI 2681 ATCGAT AT^CGAT
DrdI 3192 GACACAGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2894 CGGCCG C^GGCCG
Eam1105I 2739 GACACAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 3813 CTCTTC CTCTTC(1/4)
EclXI 2894 CGGCCG C^GGCCG
Eco47III 3594 AGCGCT AGC^GCT
Eco52I 2894 CGGCCG C^GGCCG
Eco81I 3717 CCTCAGG CC^T[ACGT]AGG
EcoRI 913 GAATTC G^AATTC
FbaI 1004 TGATCA T^GATCA
FseI 1790 GGCCGGCC GGCCGG^CC
FspI 3425 TGCGCA TGC^GCA
HincII 2870 GTTGAC GT[CT]^[GA]AC
HindII 2870 GTTGAC GT[CT]^[GA]AC
Ksp632I 3813 CTCTTC CTCTTC(1/4)
MamI 1591 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 976 CAATTG C^AATTG
MunI 976 CAATTG C^AATTG
NaeI 1791 GCCGGC GCC^GGC
NgoMIV 1791 GCCGGC G^CCGGC
NheI 727 GCTAGC G^CTAGC
PshAI 1638 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1386 ATTAAT AT^TAAT
SacI 1547 GAGCTC GAGCT^C
ScaI 1348 AGTACT AGT^ACT
SexAI 1306 ACCAGGT A^CC[AT]GGT
SnaBI 4142 TACGTA TAC^GTA
SphI 886 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 2586 GGTACC G^GTACC
3679 GGTACC
Asp718I 2586 GGTACC G^GTACC
3679 GGTACC
AspI 1749 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4599 GACAGCGTC
BbrPI 3237 CACGTG CAC^GTG
3963 CACGTG
BlpI 3263 GCTGAGC GC^T[ACGT]AGC
4188 GCTGAGC
Bpu1102I 3263 GCTGAGC GC^T[ACGT]AGC
4188 GCTGAGC
BspHI 13 TCATGA T^CATGA
4466 TCATGA
BspLU11I 663 ACATGT A^CATGT
738 ACATGT
BsrFI 1791 GCCGGC [GA]^CCGG[CT]
3762 GCCGGT
BssSI 4562 CACGAG CACGAG(-5/-1)
1699 CTCGTG
BstEII 2921 GGTGACC G^GT[ACGT]ACC
3548 GGTCACC
BstPI 2921 GGTGACC G^GT[ACGT]ACC
3548 GGTCACC
CelII 3263 GCTGAGC GC^T[ACGT]AGC
4188 GCTGAGC
Cfr10I 1791 GCCGGC [GA]^CCGG[CT]
3762 GCCGGT
DraI 1271 TTTAAA TTT^AAA
4738 TTTAAA
EcoO65I 2921 GGTGACC G^GT[ACGT]ACC
3548 GGTCACC
EcoT22I 948 ATGCAT ATGCA^T
1323 ATGCAT
KpnI 2586 GGTACC GGTAC^C
3679 GGTACC
NcoI 1524 CCATGG C^CATGG
1572 CCATGG
NsiI 948 ATGCAT ATGCA^T
1323 ATGCAT
PflFI 1749 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4599 GACAGCGTC
PmaCI 3237 CACGTG CAC^GTG
3963 CACGTG
PmlI 3237 CACGTG CAC^GTG
3963 CACGTG
PvuI 360 CGATCG CGAT^CG
2679 CGATCG
RcaI 13 TCATGA T^CATGA
4466 TCATGA
SmlI 2786 CTCAAG C^T[CT][GA]AG
4023 CTCAAG
StuI 1873 AGGCCT AGG^CCT
2831 AGGCCT
Tth111I 1749 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4599 GACAGCGTC
XbaI 1353 TCTAGA T^CTAGA
1964 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmaCI XmaI