##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BfrI BglII
BlnI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DrdI Eam1105I EcoO65I
EcoRI EcoRV EcoT22I FseI HgaI
HindIII HpaI KpnI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SfiI
SfuI SgrAI SnaBI SpeI SspBI
SspI SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
BamHI 2669 GGATCC G^GATCC
BbrPI 3370 CACGTG CAC^GTG
BciVI 3240 GGATAC GTATCC(6/5)
BclI 2227 TGATCA T^GATCA
BsaBI 3795 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2751 GAGACC GGTCTC(1/5)
BsmBI 2249 CGTCTC CGTCTC(1/5)
BspLU11I 4033 ACATGT A^CATGT
EagI 4188 CGGCCG C^GGCCG
EclXI 4188 CGGCCG C^GGCCG
Eco52I 4188 CGGCCG C^GGCCG
FbaI 2227 TGATCA T^GATCA
KspI 72 CCGCGG CCGC^GG
MamI 3795 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 2008 TTAA T^TAA
PaeR7I 1464 CTCGAG C^TCGAG
PmaCI 3370 CACGTG CAC^GTG
PmlI 3370 CACGTG CAC^GTG
SacII 72 CCGCGG CCGC^GG
SapI 1967 GCTCTTC GCTCTTC(1/4)
SbfI 1922 CCTGCAGG CCTGCA^GG
ScaI 451 AGTACT AGT^ACT
SphI 1882 GCATGC GCATG^C
Sse8387I 1922 CCTGCAGG CCTGCA^GG
Tru9I 2008 TTAA T^TAA
XhoI 1464 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AflIII 1527 ACGTGT A^C[GA][CT]GT
4033 ACATGT
AocI 1741 CCTCAGG CC^T[ACGT]AGG
3924 CCTGAGG
Aor51HI 2047 AGCGCT AGC^GCT
3166 AGCGCT
BalI 310 TGGCCA TGG^CCA
1867 TGGCCA
BcgI 1456 GCAGCAACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3148 GCACCCGCCTCG
BsaWI 1602 ACCGGA [AT]^CCGG[AT]
4787 ACCGGA
BsiEI 4188 CGGCCG CG[GA][CT]^CG
4544 CGACCG
BsrDI 2676 CATTGC GCAATG(2/0)
1030 GCAATG
Bsu36I 1741 CCTCAGG CC^T[ACGT]AGG
3924 CCTGAGG
Eco47III 2047 AGCGCT AGC^GCT
3166 AGCGCT
Eco81I 1741 CCTCAGG CC^T[ACGT]AGG
3924 CCTGAGG
HincII 537 GTCAAC GT[CT]^[GA]AC
3834 GTCAAC
HindII 537 GTCAAC GT[CT]^[GA]AC
3834 GTCAAC
MluNI 310 TGGCCA TGG^CCA
1867 TGGCCA
MscI 310 TGGCCA TGG^CCA
1867 TGGCCA
SexAI 532 ACCTGGT A^CC[AT]GGT
2167 ACCTGGT
SmlI 1464 CTCGAG C^T[CT][GA]AG
2184 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaI ApaLI
AspHI AvaI AvaII AviII BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI StuI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI