##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AclI AgeI AocI ApaI AscI
AviII BamHI BbeI BglI BsaI
BsiWI BsmI BspLU11I BsrFI Bst1107I
BstZ17I Bsu36I Cfr10I CpoI DrdI
EagI EclXI Eco52I Eco81I EcoRV
FseI FspI KasI MluI NaeI
NarI NdeI NgoMIV NheI NotI
NspI PacI PaeR7I PflMI PinAI
PmeI PshAI Psp1406I RsrII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SnaBI SphI Sse8387I
StuI SwaI Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
Acc65I 1817 GGTACC G^GTACC
AccI 2408 GTCTAC GT^[AC][GT]AC
AflII 426 CTTAAG C^TTAAG
Alw44I 1785 GTGCAC G^TGCAC
AlwNI 529 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 4214 AGCGCT AGC^GCT
ApaLI 1785 GTGCAC G^TGCAC
Asp700I 2113 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1817 GGTACC G^GTACC
AvaI 9 CCCGGG C^[CT]CG[GA]G
BanI 1817 GGTACC G^G[CT][GA]CC
BanII 510 GAGCTC G[GA]GC[CT]^C
BbrPI 2183 CACGTG CAC^GTG
BbvCI 344 GCTGAGG CCTCAGC(-5/-2)
BcgI 1787 GCACATCAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 838 TGATCA T^GATCA
BfrI 426 CTTAAG C^TTAAG
BlpI 3747 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3747 GCTGAGC GC^T[ACGT]AGC
BsaAI 2183 CACGTG [CT]AC^GT[GA]
BsiEI 71 CGATCG CG[GA][CT]^CG
BsmBI 182 GAGACG CGTCTC(1/5)
BsoBI 9 CCCGGG C^[CT]CG[GA]G
BspDI 1605 ATCGAT AT^CGAT
BspHI 3022 TCATGA T^CATGA
BsrBI 81 CCGCTC CCGCTC(-3/-3)
BssSI 2005 CTCGTG CACGAG(-5/-1)
BstAPI 3081 GCAAGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1231 TTCGAA TT^CGAA
BstEII 228 GGTCACC G^GT[ACGT]ACC
BstPI 228 GGTCACC G^GT[ACGT]ACC
CelII 3747 GCTGAGC GC^T[ACGT]AGC
ClaI 1605 ATCGAT AT^CGAT
DraIII 639 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 4214 AGCGCT AGC^GCT
EcoO65I 228 GGTCACC G^GT[ACGT]ACC
EcoRI 2966 GAATTC G^AATTC
EcoT22I 3088 ATGCAT ATGCA^T
FbaI 838 TGATCA T^GATCA
HaeII 4214 AGCGCT [GA]GCGC^[CT]
HindIII 409 AAGCTT A^AGCTT
HpaI 4169 GTTAAC GTT^AAC
KpnI 1817 GGTACC GGTAC^C
KspI 338 CCGCGG CCGC^GG
MfeI 2565 CAATTG C^AATTG
MunI 2565 CAATTG C^AATTG
NruI 166 TCGCGA TCG^CGA
NsiI 3088 ATGCAT ATGCA^T
NspV 1231 TTCGAA TT^CGAA
PmaCI 2183 CACGTG CAC^GTG
PmlI 2183 CACGTG CAC^GTG
PvuI 71 CGATCG CGAT^CG
RcaI 3022 TCATGA T^CATGA
SacI 510 GAGCTC GAGCT^C
SacII 338 CCGCGG CCGC^GG
SfuI 1231 TTCGAA TT^CGAA
SmaI 9 CCCGGG CCC^GGG
SpeI 3614 ACTAGT A^CTAGT
XmaCI 9 CCCGGG C^CCGGG
XmaI 9 CCCGGG C^CCGGG
XmnI 2113 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AatII 759 GACGTC GACGT^C
1946 GACGTC
AccIII 40 TCCGGA T^CCGGA
3962 TCCGGA
AflIII 825 ACACGT A^C[GA][CT]GT
2184 ACGTGT
AseI 1601 ATTAAT AT^TAAT
3716 ATTAAT
AsnI 1601 ATTAAT AT^TAAT
3716 ATTAAT
AspHI 510 GAGCTC G[AT]GC[AT]^C
1785 GTGCAC
AspI 1091 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1415 GACAATGTC
AvrII 3212 CCTAGG C^CTAGG
3460 CCTAGG
BbsI 1301 GAAGAC GAAGAC(2/6)
2930 GAAGAC
BciVI 497 GGATAC GTATCC(6/5)
900 GGATAC
BlnI 3212 CCTAGG C^CTAGG
3460 CCTAGG
BpuAI 1301 GAAGAC GAAGAC(2/6)
2930 GAAGAC
BsaBI 2286 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3590 GATCTTCATC
BseAI 40 TCCGGA T^CCGGA
3962 TCCGGA
BsiHKAI 510 GAGCTC G[AT]GC[AT]^C
1785 GTGCAC
Bsp1407I 4223 TGTACA T^GTACA
4462 TGTACA
BspEI 40 TCCGGA T^CCGGA
3962 TCCGGA
BsrGI 4223 TGTACA T^GTACA
4462 TGTACA
BssHII 118 GCGCGC G^CGCGC
144 GCGCGC
BstXI 3980 CCATGCTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4377 CCATGTTCCTGG
FauI 28 CCCGC CCCGC(4/6)
340 GCGGG
HgaI 2591 GACGC GACGC(5/10)
2222 GCGTC
MamI 2286 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3590 GATCTTCATC
MroI 40 TCCGGA T^CCGGA
3962 TCCGGA
PflFI 1091 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1415 GACAATGTC
PpuMI 1396 GGGACCT [GA]G^G[AT]CC[CT]
3623 AGGTCCT
PshBI 1601 ATTAAT AT^TAAT
3716 ATTAAT
SspBI 4223 TGTACA T^GTACA
4462 TGTACA
Tth111I 1091 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1415 GACAATGTC
XcmI 1383 CCAGCAAAAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2739 CCAAAAGAATATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AhdI AluI AlwI ApoI AspEI
AvaII BalI BbvI BcnI BfaI
BglII BmyI BpmI BsaHI BsaJI
BsaWI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
Eam1105I EarI EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII