##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AccI AclI AcsI AgeI Alw44I
ApaLI ApoI AscI AseI AsnI
AviII BamHI BcgI BsaBI BsiEI
BsiWI Bsp1407I BspDI BspHI BspLU11I
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI DraIII EagI
EclXI Eco52I EcoRI FseI FspI
HincII HindII HpaI MamI MfeI
MluI MunI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI SspBI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 3710 GACGTC GACGT^C
Acc65I 1552 GGTACC G^GTACC
AflII 1400 CTTAAG C^TTAAG
AflIII 3851 ACACGT A^C[GA][CT]GT
Aor51HI 2996 AGCGCT AGC^GCT
Asp700I 2873 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1552 GGTACC G^GTACC
AspI 1086 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 3523 GGCGCC GGCGC^C
BbrPI 3852 CACGTG CAC^GTG
BciVI 4319 GTATCC GTATCC(6/5)
BclI 392 TGATCA T^GATCA
BfrI 1400 CTTAAG C^TTAAG
BglII 885 AGATCT A^GATCT
BsmBI 2739 CGTCTC CGTCTC(1/5)
BssSI 2706 CTCGTG CACGAG(-5/-1)
BstEII 2160 GGTTACC G^GT[ACGT]ACC
BstPI 2160 GGTTACC G^GT[ACGT]ACC
DrdI 2381 GACACAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2996 AGCGCT AGC^GCT
EcoO65I 2160 GGTTACC G^GT[ACGT]ACC
EcoRV 4464 GATATC GAT^ATC
EcoT22I 4548 ATGCAT ATGCA^T
FbaI 392 TGATCA T^GATCA
HindIII 4158 AAGCTT A^AGCTT
KasI 3523 GGCGCC G^GCGCC
KpnI 1552 GGTACC GGTAC^C
KspI 2214 CCGCGG CCGC^GG
NarI 3523 GGCGCC GG^CGCC
NdeI 4594 CATATG CA^TATG
NheI 1385 GCTAGC G^CTAGC
NsiI 4548 ATGCAT ATGCA^T
NspI 1331 GCATGT [GA]CATG^[CT]
PflFI 1086 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3852 CACGTG CAC^GTG
PmlI 3852 CACGTG CAC^GTG
SacII 2214 CCGCGG CCGC^GG
SapI 1491 GCTCTTC GCTCTTC(1/4)
SbfI 827 CCTGCAGG CCTGCA^GG
SfoI 3523 GGCGCC GGC^GCC
SmaI 2861 CCCGGG CCC^GGG
Sse8387I 827 CCTGCAGG CCTGCA^GG
SspI 1998 AATATT AAT^ATT
Tth111I 1086 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2861 CCCGGG C^CCGGG
XmaI 2861 CCCGGG C^CCGGG
XmnI 2873 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 505 CGCG CG^CG
2215 CGCG
AccIII 2032 TCCGGA T^CCGGA
3961 TCCGGA
AcyI 3523 GGCGCC G[GA]^CG[CT]C
3710 GACGTC
AhdI 1693 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3177 GACCAGCTGTC
AspEI 1693 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3177 GACCAGCTGTC
AvrII 666 CCTAGG C^CTAGG
4276 CCTAGG
BbsI 3930 GAAGAC GAAGAC(2/6)
1177 GTCTTC
BlnI 666 CCTAGG C^CTAGG
4276 CCTAGG
BpuAI 3930 GAAGAC GAAGAC(2/6)
1177 GTCTTC
BsaAI 1746 TACGTG [CT]AC^GT[GA]
3852 CACGTG
BsaHI 3523 GGCGCC G[GA]^CG[CT]C
3710 GACGTC
BsaWI 2032 TCCGGA [AT]^CCGG[AT]
3961 TCCGGA
BseAI 2032 TCCGGA T^CCGGA
3961 TCCGGA
BspEI 2032 TCCGGA T^CCGGA
3961 TCCGGA
BstUI 505 CGCG CG^CG
2215 CGCG
Eam1105I 1693 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3177 GACCAGCTGTC
EcoNI 350 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2576 CCTGTCAGAGG
HgaI 3809 GCGTC GACGC(5/10)
4217 GCGTC
Hin1I 3523 GGCGCC G[GA]^CG[CT]C
3710 GACGTC
MroI 2032 TCCGGA T^CCGGA
3961 TCCGGA
MvnI 505 CGCG CG^CG
2215 CGCG
XcmI 1837 CCACAGGGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3463 CCACAGACACCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII