##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspI AviII AvrII BciVI
BclI BfrI BlnI BsaBI BsiWI
Bsp1407I BspHI BsrBI BsrGI Bst1107I
BstBI BstZ17I CpoI DraIII DrdI
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI MamI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I RcaI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AccIII 3544 TCCGGA T^CCGGA
AcsI 3126 GAATTT [GA]^AATT[CT]
Aor51HI 2791 AGCGCT AGC^GCT
ApaI 1911 GGGCCC GGGCC^C
ApoI 3126 GAATTT [GA]^AATT[CT]
Asp700I 2616 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 108 GGATCC G^GATCC
BcgI 3650 CGAAGCCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BseAI 3544 TCCGGA T^CCGGA
BsmI 2627 GAATGC GAATGC(1/-1)
BspDI 3003 ATCGAT AT^CGAT
BspEI 3544 TCCGGA T^CCGGA
BspLU11I 568 ACATGT A^CATGT
BssHII 2631 GCGCGC G^CGCGC
ClaI 3003 ATCGAT AT^CGAT
DraI 402 TTTAAA TTT^AAA
EagI 1762 CGGCCG C^GGCCG
EclXI 1762 CGGCCG C^GGCCG
Eco47III 2791 AGCGCT AGC^GCT
Eco52I 1762 CGGCCG C^GGCCG
KspI 1185 CCGCGG CCGC^GG
MroI 3544 TCCGGA T^CCGGA
PshAI 897 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 3005 CGATCG CGAT^CG
SacI 3714 GAGCTC GAGCT^C
SacII 1185 CCGCGG CCGC^GG
SspI 3604 AATATT AAT^ATT
StuI 1654 AGGCCT AGG^CCT
XmnI 2616 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AhdI 855 GACCCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
948 GACAGCAAGTC
AocI 1225 CCTCAGG CC^T[ACGT]AGG
2952 CCTGAGG
AspEI 855 GACCCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
948 GACAGCAAGTC
BbeI 1867 GGCGCC GGCGC^C
2514 GGCGCC
BbrPI 2728 CACGTG CAC^GTG
3035 CACGTG
BbvCI 599 GCTGAGG CCTCAGC(-5/-2)
3336 GCTGAGG
BglII 758 AGATCT A^GATCT
2188 AGATCT
BsaAI 2728 CACGTG [CT]AC^GT[GA]
3035 CACGTG
BsaI 1491 GAGACC GGTCTC(1/5)
1536 GAGACC
BsaWI 3088 TCCGGT [AT]^CCGG[AT]
3544 TCCGGA
BsrDI 3656 CATTGC GCAATG(2/0)
1468 GCAATG
BssSI 304 CACGAG CACGAG(-5/-1)
1201 CACGAG
Bsu36I 1225 CCTCAGG CC^T[ACGT]AGG
2952 CCTGAGG
Eam1105I 855 GACCCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
948 GACAGCAAGTC
Eco81I 1225 CCTCAGG CC^T[ACGT]AGG
2952 CCTGAGG
KasI 1867 GGCGCC G^GCGCC
2514 GGCGCC
NaeI 1105 GCCGGC GCC^GGC
1764 GCCGGC
NarI 1867 GGCGCC GG^CGCC
2514 GGCGCC
NcoI 1143 CCATGG C^CATGG
2660 CCATGG
NgoMIV 1105 GCCGGC G^CCGGC
1764 GCCGGC
PmaCI 2728 CACGTG CAC^GTG
3035 CACGTG
PmlI 2728 CACGTG CAC^GTG
3035 CACGTG
SfoI 1867 GGCGCC GGC^GCC
2514 GGCGCC
SphI 2159 GCATGC GCATG^C
2497 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI