################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII AccIII AclI AflII Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I AspI BamHI BbrPI BciVI BfrI BglII BsaBI BseAI BsiWI BsmBI BsmI BspDI BspEI BsrDI BstBI ClaI CpoI DraI DrdI Eco47III EcoRV EcoT22I FseI HincII HindII HindIII HpaI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PflFI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SalI ScaI SexAI SfuI SgrAI SnaBI SpeI SphI SspI SwaI Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 2972 GGTACC G^GTACC AccI 3133 GTATAC GT^[AC][GT]AC AcsI 356 GAATTC [GA]^AATT[CT] AflIII 131 ACATGT A^C[GA][CT]GT AlwI 3632 GATCC GGATC(4/5) ApoI 356 GAATTC [GA]^AATT[CT] Asp718I 2972 GGTACC G^GTACC AviII 2438 TGCGCA TGC^GCA AvrII 3868 CCTAGG C^CTAGG BbeI 2891 GGCGCC GGCGC^C BcgI 3051 CGACGTGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 631 TGATCA T^GATCA BlnI 3868 CCTAGG C^CTAGG BsaAI 3136 TACGTG [CT]AC^GT[GA] Bsp1407I 2758 TGTACA T^GTACA BspHI 2067 TCATGA T^CATGA BspLU11I 131 ACATGT A^CATGT BsrGI 2758 TGTACA T^GTACA BssHII 3746 GCGCGC G^CGCGC Bst1107I 3133 GTATAC GTA^TAC BstYI 3631 AGATCC [GA]^GATC[CT] BstZ17I 3133 GTATAC GTA^TAC EagI 3764 CGGCCG C^GGCCG EclXI 3764 CGGCCG C^GGCCG Eco52I 3764 CGGCCG C^GGCCG EcoRI 356 GAATTC G^AATTC FbaI 631 TGATCA T^GATCA FspI 2438 TGCGCA TGC^GCA KasI 2891 GGCGCC G^GCGCC KpnI 2972 GGTACC GGTAC^C KspI 47 CCGCGG CCGC^GG MflI 3631 AGATCC [GA]^GATC[CT] MseI 3389 TTAA T^TAA NaeI 1089 GCCGGC GCC^GGC NarI 2891 GGCGCC GG^CGCC NcoI 2106 CCATGG C^CATGG NgoMIV 1089 GCCGGC G^CCGGC PshAI 1664 GACTTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 2067 TCATGA T^CATGA SacII 47 CCGCGG CCGC^GG SbfI 1818 CCTGCAGG CCTGCA^GG SfoI 2891 GGCGCC GGC^GCC Sse8387I 1818 CCTGCAGG CCTGCA^GG SspBI 2758 TGTACA T^GTACA Tru9I 3389 TTAA T^TAA Tsp509I 357 AATT ^AATT XhoII 3631 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AgeI 55 ACCGGT A^CCGGT 2541 ACCGGT AhdI 54 GACCGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 233 GACGCAGGGTC AocI 1159 CCTGAGG CC^T[ACGT]AGG 1758 CCTGAGG AspEI 54 GACCGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 233 GACGCAGGGTC BalI 3272 TGGCCA TGG^CCA 3546 TGGCCA BbsI 1438 GTCTTC GAAGAC(2/6) 3100 GTCTTC BbvCI 386 CCTCAGC CCTCAGC(-5/-2) 3229 GCTGAGG BfaI 1798 CTAG C^TAG 3869 CTAG BglI 401 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1042 GCCCATCTGGC BpuAI 1438 GTCTTC GAAGAC(2/6) 3100 GTCTTC BsiEI 53 CGACCG CG[GA][CT]^CG 3764 CGGCCG BssSI 377 CACGAG CACGAG(-5/-1) 3603 CACGAG Bsu36I 1159 CCTGAGG CC^T[ACGT]AGG 1758 CCTGAGG DraIII 983 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG 2863 CACCGTGTG Eam1105I 54 GACCGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 233 GACGCAGGGTC Eco81I 1159 CCTGAGG CC^T[ACGT]AGG 1758 CCTGAGG MaeI 1798 CTAG C^TAG 3869 CTAG MluNI 3272 TGGCCA TGG^CCA 3546 TGGCCA MscI 3272 TGGCCA TGG^CCA 3546 TGGCCA PaeR7I 877 CTCGAG C^TCGAG 1940 CTCGAG PinAI 55 ACCGGT A^CCGGT 2541 ACCGGT SapI 2145 GCTCTTC GCTCTTC(1/4) 3644 GCTCTTC SfiI 400 GGCCACCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1041 GGCCCATCTGGCC SmaI 93 CCCGGG CCC^GGG 293 CCCGGG SmlI 877 CTCGAG C^T[CT][GA]AG 1940 CTCGAG XhoI 877 CTCGAG C^TCGAG 1940 CTCGAG XmaCI 93 CCCGGG C^CCGGG 293 CCCGGG XmaI 93 CCCGGG C^CCGGG 293 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbvI BcnI BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I Van91I XcmI