##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccIII AclI AflII Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspI BamHI BbrPI BciVI
BfrI BglII BsaBI BseAI BsiWI
BsmBI BsmI BspDI BspEI BsrDI
BstBI ClaI CpoI DraI DrdI
Eco47III EcoRV EcoT22I FseI HincII
HindII HindIII HpaI MamI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SalI ScaI
SexAI SfuI SgrAI SnaBI SpeI
SphI SspI SwaI Tth111I XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 2972 GGTACC G^GTACC
AccI 3133 GTATAC GT^[AC][GT]AC
AcsI 356 GAATTC [GA]^AATT[CT]
AflIII 131 ACATGT A^C[GA][CT]GT
AlwI 3632 GATCC GGATC(4/5)
ApoI 356 GAATTC [GA]^AATT[CT]
Asp718I 2972 GGTACC G^GTACC
AviII 2438 TGCGCA TGC^GCA
AvrII 3868 CCTAGG C^CTAGG
BbeI 2891 GGCGCC GGCGC^C
BcgI 3051 CGACGTGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 631 TGATCA T^GATCA
BlnI 3868 CCTAGG C^CTAGG
BsaAI 3136 TACGTG [CT]AC^GT[GA]
Bsp1407I 2758 TGTACA T^GTACA
BspHI 2067 TCATGA T^CATGA
BspLU11I 131 ACATGT A^CATGT
BsrGI 2758 TGTACA T^GTACA
BssHII 3746 GCGCGC G^CGCGC
Bst1107I 3133 GTATAC GTA^TAC
BstYI 3631 AGATCC [GA]^GATC[CT]
BstZ17I 3133 GTATAC GTA^TAC
EagI 3764 CGGCCG C^GGCCG
EclXI 3764 CGGCCG C^GGCCG
Eco52I 3764 CGGCCG C^GGCCG
EcoRI 356 GAATTC G^AATTC
FbaI 631 TGATCA T^GATCA
FspI 2438 TGCGCA TGC^GCA
KasI 2891 GGCGCC G^GCGCC
KpnI 2972 GGTACC GGTAC^C
KspI 47 CCGCGG CCGC^GG
MflI 3631 AGATCC [GA]^GATC[CT]
MseI 3389 TTAA T^TAA
NaeI 1089 GCCGGC GCC^GGC
NarI 2891 GGCGCC GG^CGCC
NcoI 2106 CCATGG C^CATGG
NgoMIV 1089 GCCGGC G^CCGGC
PshAI 1664 GACTTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2067 TCATGA T^CATGA
SacII 47 CCGCGG CCGC^GG
SbfI 1818 CCTGCAGG CCTGCA^GG
SfoI 2891 GGCGCC GGC^GCC
Sse8387I 1818 CCTGCAGG CCTGCA^GG
SspBI 2758 TGTACA T^GTACA
Tru9I 3389 TTAA T^TAA
Tsp509I 357 AATT ^AATT
XhoII 3631 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AgeI 55 ACCGGT A^CCGGT
2541 ACCGGT
AhdI 54 GACCGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
233 GACGCAGGGTC
AocI 1159 CCTGAGG CC^T[ACGT]AGG
1758 CCTGAGG
AspEI 54 GACCGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
233 GACGCAGGGTC
BalI 3272 TGGCCA TGG^CCA
3546 TGGCCA
BbsI 1438 GTCTTC GAAGAC(2/6)
3100 GTCTTC
BbvCI 386 CCTCAGC CCTCAGC(-5/-2)
3229 GCTGAGG
BfaI 1798 CTAG C^TAG
3869 CTAG
BglI 401 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1042 GCCCATCTGGC
BpuAI 1438 GTCTTC GAAGAC(2/6)
3100 GTCTTC
BsiEI 53 CGACCG CG[GA][CT]^CG
3764 CGGCCG
BssSI 377 CACGAG CACGAG(-5/-1)
3603 CACGAG
Bsu36I 1159 CCTGAGG CC^T[ACGT]AGG
1758 CCTGAGG
DraIII 983 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
2863 CACCGTGTG
Eam1105I 54 GACCGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
233 GACGCAGGGTC
Eco81I 1159 CCTGAGG CC^T[ACGT]AGG
1758 CCTGAGG
MaeI 1798 CTAG C^TAG
3869 CTAG
MluNI 3272 TGGCCA TGG^CCA
3546 TGGCCA
MscI 3272 TGGCCA TGG^CCA
3546 TGGCCA
PaeR7I 877 CTCGAG C^TCGAG
1940 CTCGAG
PinAI 55 ACCGGT A^CCGGT
2541 ACCGGT
SapI 2145 GCTCTTC GCTCTTC(1/4)
3644 GCTCTTC
SfiI 400 GGCCACCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1041 GGCCCATCTGGCC
SmaI 93 CCCGGG CCC^GGG
293 CCCGGG
SmlI 877 CTCGAG C^T[CT][GA]AG
1940 CTCGAG
XhoI 877 CTCGAG C^TCGAG
1940 CTCGAG
XmaCI 93 CCCGGG C^CCGGG
293 CCCGGG
XmaI 93 CCCGGG C^CCGGG
293 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbvI BcnI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
Van91I XcmI