##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AccIII AhdI AscI AseI AsnI
AspEI AspI AviII BamHI BcgI
BciVI BglII BsaBI BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BsrDI
BsrGI BstAPI BstBI BstEII BstPI
ClaI CpoI DraIII Eam1105I EcoO65I
EcoRI EcoT22I FspI HpaI MamI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PmeI PshAI
PshBI PvuI RcaI RsrII SalI
SbfI ScaI SfuI SgrAI SnaBI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 421 GGTACC G^GTACC
AcsI 3800 AAATTT [GA]^AATT[CT]
AflII 3297 CTTAAG C^TTAAG
AgeI 1374 ACCGGT A^CCGGT
AocI 2494 CCTCAGG CC^T[ACGT]AGG
ApoI 3800 AAATTT [GA]^AATT[CT]
Asp700I 3799 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 421 GGTACC G^GTACC
BbrPI 2197 CACGTG CAC^GTG
BclI 4049 TGATCA T^GATCA
BfrI 3297 CTTAAG C^TTAAG
BlpI 3615 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3615 GCTGAGC GC^T[ACGT]AGC
BsaAI 2197 CACGTG [CT]AC^GT[GA]
BsaI 3229 GAGACC GGTCTC(1/5)
BsgI 1764 CTGCAC GTGCAG(16/14)
BsmBI 627 GAGACG CGTCTC(1/5)
BsmI 967 GCATTC GAATGC(1/-1)
BsrBI 1532 GAGCGG CCGCTC(-3/-3)
Bst1107I 4024 GTATAC GTA^TAC
BstYI 1600 AGATCC [GA]^GATC[CT]
BstZ17I 4024 GTATAC GTA^TAC
Bsu36I 2494 CCTCAGG CC^T[ACGT]AGG
CelII 3615 GCTGAGC GC^T[ACGT]AGC
Eco81I 2494 CCTCAGG CC^T[ACGT]AGG
EcoRV 3730 GATATC GAT^ATC
FbaI 4049 TGATCA T^GATCA
FseI 2043 GGCCGGCC GGCCGG^CC
HindIII 3818 AAGCTT A^AGCTT
KpnI 421 GGTACC GGTAC^C
MflI 1600 AGATCC [GA]^GATC[CT]
NaeI 2044 GCCGGC GCC^GGC
NcoI 986 CCATGG C^CATGG
NgoMIV 2044 GCCGGC G^CCGGC
PinAI 1374 ACCGGT A^CCGGT
PmaCI 2197 CACGTG CAC^GTG
PmlI 2197 CACGTG CAC^GTG
SapI 1586 GCTCTTC GCTCTTC(1/4)
SexAI 494 ACCTGGT A^CC[AT]GGT
SfiI 4145 GGCCTGTAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 2626 ACTAGT A^CTAGT
XhoII 1600 AGATCC [GA]^GATC[CT]
XmnI 3799 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AatII 1117 GACGTC GACGT^C
1890 GACGTC
AclI 584 AACGTT AA^CGTT
3373 AACGTT
Alw44I 1759 GTGCAC G^TGCAC
1931 GTGCAC
AlwNI 504 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
521 CAGGGGCTG
Aor51HI 113 AGCGCT AGC^GCT
206 AGCGCT
ApaI 1240 GGGCCC GGGCC^C
2151 GGGCCC
ApaLI 1759 GTGCAC G^TGCAC
1931 GTGCAC
BbvCI 612 GCTGAGG CCTCAGC(-5/-2)
4261 GCTGAGG
BsaWI 978 TCCGGT [AT]^CCGG[AT]
1374 ACCGGT
BspLU11I 3933 ACATGT A^CATGT
4289 ACATGT
BssHII 2016 GCGCGC G^CGCGC
2018 GCGCGC
BssSI 1767 CACGAG CACGAG(-5/-1)
1984 CTCGTG
DrdI 629 GACGAATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1515 GACAGCGGCGTC
EagI 164 CGGCCG C^GGCCG
1652 CGGCCG
EclXI 164 CGGCCG C^GGCCG
1652 CGGCCG
Eco47III 113 AGCGCT AGC^GCT
206 AGCGCT
Eco52I 164 CGGCCG C^GGCCG
1652 CGGCCG
HincII 555 GTTGAC GT[CT]^[GA]AC
1042 GTCAAC
HindII 555 GTTGAC GT[CT]^[GA]AC
1042 GTCAAC
MslI 311 CACCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4196 CACAGGCATG
Psp1406I 584 AACGTT AA^CGTT
3373 AACGTT
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI AlwI AspHI AvaI
AvaII AvrII BalI BanI BanII
BbeI BbsI BbvI BcnI BfaI
BglI BlnI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XmaCI XmaI