################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AccIII AhdI AscI AseI AsnI AspEI AspI AviII BamHI BcgI BciVI BglII BsaBI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BsrDI BsrGI BstAPI BstBI BstEII BstPI ClaI CpoI DraIII Eam1105I EcoO65I EcoRI EcoT22I FspI HpaI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmeI PshAI PshBI PvuI RcaI RsrII SalI SbfI ScaI SfuI SgrAI SnaBI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 421 GGTACC G^GTACC AcsI 3800 AAATTT [GA]^AATT[CT] AflII 3297 CTTAAG C^TTAAG AgeI 1374 ACCGGT A^CCGGT AocI 2494 CCTCAGG CC^T[ACGT]AGG ApoI 3800 AAATTT [GA]^AATT[CT] Asp700I 3799 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 421 GGTACC G^GTACC BbrPI 2197 CACGTG CAC^GTG BclI 4049 TGATCA T^GATCA BfrI 3297 CTTAAG C^TTAAG BlpI 3615 GCTGAGC GC^T[ACGT]AGC Bpu1102I 3615 GCTGAGC GC^T[ACGT]AGC BsaAI 2197 CACGTG [CT]AC^GT[GA] BsaI 3229 GAGACC GGTCTC(1/5) BsgI 1764 CTGCAC GTGCAG(16/14) BsmBI 627 GAGACG CGTCTC(1/5) BsmI 967 GCATTC GAATGC(1/-1) BsrBI 1532 GAGCGG CCGCTC(-3/-3) Bst1107I 4024 GTATAC GTA^TAC BstYI 1600 AGATCC [GA]^GATC[CT] BstZ17I 4024 GTATAC GTA^TAC Bsu36I 2494 CCTCAGG CC^T[ACGT]AGG CelII 3615 GCTGAGC GC^T[ACGT]AGC Eco81I 2494 CCTCAGG CC^T[ACGT]AGG EcoRV 3730 GATATC GAT^ATC FbaI 4049 TGATCA T^GATCA FseI 2043 GGCCGGCC GGCCGG^CC HindIII 3818 AAGCTT A^AGCTT KpnI 421 GGTACC GGTAC^C MflI 1600 AGATCC [GA]^GATC[CT] NaeI 2044 GCCGGC GCC^GGC NcoI 986 CCATGG C^CATGG NgoMIV 2044 GCCGGC G^CCGGC PinAI 1374 ACCGGT A^CCGGT PmaCI 2197 CACGTG CAC^GTG PmlI 2197 CACGTG CAC^GTG SapI 1586 GCTCTTC GCTCTTC(1/4) SexAI 494 ACCTGGT A^CC[AT]GGT SfiI 4145 GGCCTGTAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SpeI 2626 ACTAGT A^CTAGT XhoII 1600 AGATCC [GA]^GATC[CT] XmnI 3799 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AatII 1117 GACGTC GACGT^C 1890 GACGTC AclI 584 AACGTT AA^CGTT 3373 AACGTT Alw44I 1759 GTGCAC G^TGCAC 1931 GTGCAC AlwNI 504 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG 521 CAGGGGCTG Aor51HI 113 AGCGCT AGC^GCT 206 AGCGCT ApaI 1240 GGGCCC GGGCC^C 2151 GGGCCC ApaLI 1759 GTGCAC G^TGCAC 1931 GTGCAC BbvCI 612 GCTGAGG CCTCAGC(-5/-2) 4261 GCTGAGG BsaWI 978 TCCGGT [AT]^CCGG[AT] 1374 ACCGGT BspLU11I 3933 ACATGT A^CATGT 4289 ACATGT BssHII 2016 GCGCGC G^CGCGC 2018 GCGCGC BssSI 1767 CACGAG CACGAG(-5/-1) 1984 CTCGTG DrdI 629 GACGAATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1515 GACAGCGGCGTC EagI 164 CGGCCG C^GGCCG 1652 CGGCCG EclXI 164 CGGCCG C^GGCCG 1652 CGGCCG Eco47III 113 AGCGCT AGC^GCT 206 AGCGCT Eco52I 164 CGGCCG C^GGCCG 1652 CGGCCG HincII 555 GTTGAC GT[CT]^[GA]AC 1042 GTCAAC HindII 555 GTTGAC GT[CT]^[GA]AC 1042 GTCAAC MslI 311 CACCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 4196 CACAGGCATG Psp1406I 584 AACGTT AA^CGTT 3373 AACGTT ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AflIII AluI AlwI AspHI AvaI AvaII AvrII BalI BanI BanII BbeI BbsI BbvI BcnI BfaI BglI BlnI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XmaCI XmaI