##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AviII BclI BglII BsaBI BsaWI
BseAI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI Eam1105I Eco47III
EcoRV FbaI FseI FspI HindIII
HpaI KspI MamI MfeI MluI
MroI MunI NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SbfI SexAI SfiI SfuI SgrAI
SnaBI Sse8387I SspBI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 1333 GGTACC G^GTACC
AcsI 1912 GAATTC [GA]^AATT[CT]
AflII 487 CTTAAG C^TTAAG
AflIII 21 ACACGT A^C[GA][CT]GT
Alw44I 4050 GTGCAC G^TGCAC
ApaLI 4050 GTGCAC G^TGCAC
ApoI 1912 GAATTC [GA]^AATT[CT]
Asp718I 1333 GGTACC G^GTACC
AspI 2413 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3436 CCTAGG C^CTAGG
BbrPI 22 CACGTG CAC^GTG
BcgI 3449 CGACAGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 487 CTTAAG C^TTAAG
BlnI 3436 CCTAGG C^CTAGG
BsaAI 22 CACGTG [CT]AC^GT[GA]
BsmBI 2454 GAGACG CGTCTC(1/5)
BspHI 2693 TCATGA T^CATGA
BssSI 426 CTCGTG CACGAG(-5/-1)
BstEII 792 GGTGACC G^GT[ACGT]ACC
BstPI 792 GGTGACC G^GT[ACGT]ACC
DrdI 2477 GACCTGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 792 GGTGACC G^GT[ACGT]ACC
EcoRI 1912 GAATTC G^AATTC
EcoT22I 6 ATGCAT ATGCA^T
HincII 2831 GTCGAC GT[CT]^[GA]AC
HindII 2831 GTCGAC GT[CT]^[GA]AC
KpnI 1333 GGTACC GGTAC^C
NaeI 1317 GCCGGC GCC^GGC
NcoI 884 CCATGG C^CATGG
NdeI 3652 CATATG CA^TATG
NgoMIV 1317 GCCGGC G^CCGGC
NsiI 6 ATGCAT ATGCA^T
PflFI 2413 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 22 CACGTG CAC^GTG
PmlI 22 CACGTG CAC^GTG
PshAI 3273 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2693 TCATGA T^CATGA
SalI 2831 GTCGAC G^TCGAC
ScaI 353 AGTACT AGT^ACT
SpeI 3002 ACTAGT A^CTAGT
SwaI 3994 ATTTAAAT ATTT^AAAT
TaqI 2832 TCGA T^CGA
Tth111I 2413 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 2832 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Asp700I 735 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3892 GAACCCCTTC
BamHI 975 GGATCC G^GATCC
1702 GGATCC
BbsI 1398 GAAGAC GAAGAC(2/6)
1732 GTCTTC
BciVI 2349 GTATCC GTATCC(6/5)
3233 GTATCC
BpuAI 1398 GAAGAC GAAGAC(2/6)
1732 GTCTTC
BsmI 772 GAATGC GAATGC(1/-1)
3735 GCATTC
BsrBI 122 CCGCTC CCGCTC(-3/-3)
1832 GAGCGG
BsrDI 2706 CATTGC GCAATG(2/0)
1135 GCAATG
DsaI 884 CCATGG C^C[GA][CT]GG
1944 CCGTGG
MaeII 23 ACGT A^CGT
3402 ACGT
MslI 2524 CACCTAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3223 CACCTGCATG
NspI 4 GCATGC [GA]CATG^[CT]
1238 GCATGC
PvuII 233 CAGCTG CAG^CTG
574 CAGCTG
SacI 2023 GAGCTC GAGCT^C
3055 GAGCTC
SapI 600 GAAGAGC GCTCTTC(1/4)
625 GAAGAGC
SmaI 78 CCCGGG CCC^GGG
1108 CCCGGG
SmlI 487 CTTAAG C^T[CT][GA]AG
2168 CTTGAG
SphI 4 GCATGC GCATG^C
1238 GCATGC
SspI 736 AATATT AAT^ATT
3769 AATATT
XmaCI 78 CCCGGG C^CCGGG
1108 CCCGGG
XmaI 78 CCCGGG C^CCGGG
1108 CCCGGG
XmnI 735 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3892 GAACCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
EaeI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XcmI XhoII