##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
Acc65I AclI AflII AgeI AscI
AseI AsnI Asp718I AspI AvrII
BcgI BfrI BglII BlnI BsaBI
BsiWI BsmI BspDI BspHI BspLU11I
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I ClaI CpoI
DraI EcoO65I EcoRI EcoRV EcoT22I
FseI HindIII HpaI KpnI MamI
MfeI MluI MunI NdeI NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SfiI SfuI SnaBI SpeI
Sse8387I SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 854 GACGTC GACGT^C
AccI 3041 GTAGAC GT^[AC][GT]AC
AccIII 2114 TCCGGA T^CCGGA
AcsI 2714 GAATTT [GA]^AATT[CT]
AflIII 457 ACGTGT A^C[GA][CT]GT
AhdI 1188 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 279 GTGCAC G^TGCAC
Aor51HI 1749 AGCGCT AGC^GCT
ApaLI 279 GTGCAC G^TGCAC
ApoI 2714 GAATTT [GA]^AATT[CT]
AspEI 1188 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1813 TGCGCA TGC^GCA
BalI 955 TGGCCA TGG^CCA
BbsI 1950 GTCTTC GAAGAC(2/6)
BciVI 2174 GTATCC GTATCC(6/5)
BclI 3319 TGATCA T^GATCA
BpuAI 1950 GTCTTC GAAGAC(2/6)
BsaWI 2114 TCCGGA [AT]^CCGG[AT]
BseAI 2114 TCCGGA T^CCGGA
BsmBI 1785 GAGACG CGTCTC(1/5)
Bsp1407I 460 TGTACA T^GTACA
BspEI 2114 TCCGGA T^CCGGA
BsrGI 460 TGTACA T^GTACA
EagI 2302 CGGCCG C^GGCCG
Eam1105I 1188 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2302 CGGCCG C^GGCCG
Eco47III 1749 AGCGCT AGC^GCT
Eco52I 2302 CGGCCG C^GGCCG
FbaI 3319 TGATCA T^GATCA
FspI 1813 TGCGCA TGC^GCA
MluNI 955 TGGCCA TGG^CCA
MroI 2114 TCCGGA T^CCGGA
MscI 955 TGGCCA TGG^CCA
NheI 3162 GCTAGC G^CTAGC
PaeR7I 3026 CTCGAG C^TCGAG
SexAI 1403 ACCTGGT A^CC[AT]GGT
SspBI 460 TGTACA T^GTACA
XhoI 3026 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AocI 1207 CCTCAGG CC^T[ACGT]AGG
3467 CCTGAGG
Asp700I 1727 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2711 GAAGAATTTC
BbrPI 456 CACGTG CAC^GTG
3057 CACGTG
BbvCI 184 CCTCAGC CCTCAGC(-5/-2)
1663 CCTCAGC
BsiEI 575 CGACCG CG[GA][CT]^CG
2302 CGGCCG
BsrDI 475 CATTGC GCAATG(2/0)
325 GCAATG
Bsu36I 1207 CCTCAGG CC^T[ACGT]AGG
3467 CCTGAGG
EaeI 955 TGGCCA [CT]^GGCC[GA]
2302 CGGCCG
Eco81I 1207 CCTCAGG CC^T[ACGT]AGG
3467 CCTGAGG
HincII 600 GTCAAC GT[CT]^[GA]AC
1680 GTCAAC
HindII 600 GTCAAC GT[CT]^[GA]AC
1680 GTCAAC
NaeI 1826 GCCGGC GCC^GGC
2796 GCCGGC
NcoI 952 CCATGG C^CATGG
1122 CCATGG
NgoMIV 1826 GCCGGC G^CCGGC
2796 GCCGGC
NspI 412 GCATGC [GA]CATG^[CT]
2852 GCATGC
PflMI 2316 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2917 CCAAGGCCTGG
PmaCI 456 CACGTG CAC^GTG
3057 CACGTG
PmlI 456 CACGTG CAC^GTG
3057 CACGTG
SapI 35 GAAGAGC GCTCTTC(1/4)
2394 GCTCTTC
SgrAI 1825 CGCCGGCG C[GA]^CCGG[CT]G
2137 CGCCGGTG
SphI 412 GCATGC GCATG^C
2852 GCATGC
SspI 2619 AATATT AAT^ATT
2962 AATATT
Van91I 2316 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2917 CCAAGGCCTGG
XmnI 1727 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2711 GAAGAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BamHI BanI BanII BbeI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DrdI DsaI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SacI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI