##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AscI AseI AsnI Asp718I AviII
AvrII BfrI BlnI BlpI Bpu1102I
BsaBI BseAI BsiWI Bsp1407I BspEI
BspHI BsrBI BsrGI BssHII Bst1107I
BstBI BstZ17I CelII CpoI EcoRV
FseI FspI HindIII HpaI KpnI
KspI MamI MfeI MluI MroI
MunI NcoI NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI PvuI RcaI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 2827 GTCTAC GT^[AC][GT]AC
AclI 3758 AACGTT AA^CGTT
AhdI 1446 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2500 GTGCAC G^TGCAC
ApaLI 2500 GTGCAC G^TGCAC
Asp700I 3780 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1446 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 2800 CCTCAGC CCTCAGC(-5/-2)
BcgI 3453 CGACAGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1736 GTATCC GTATCC(6/5)
BglII 2765 AGATCT A^GATCT
BsiEI 17 CGGCCG CG[GA][CT]^CG
BspDI 2821 ATCGAT AT^CGAT
BspLU11I 524 ACATGT A^CATGT
BsrDI 270 CATTGC GCAATG(2/0)
BstEII 2703 GGTGACC G^GT[ACGT]ACC
BstPI 2703 GGTGACC G^GT[ACGT]ACC
ClaI 2821 ATCGAT AT^CGAT
DraI 1033 TTTAAA TTT^AAA
DraIII 2078 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 739 GACTGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 17 CGGCCG C^GGCCG
Eam1105I 1446 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 17 CGGCCG C^GGCCG
Eco52I 17 CGGCCG C^GGCCG
EcoO65I 2703 GGTGACC G^GT[ACGT]ACC
EcoT22I 2959 ATGCAT ATGCA^T
HincII 3439 GTCAAC GT[CT]^[GA]AC
HindII 3439 GTCAAC GT[CT]^[GA]AC
NaeI 1837 GCCGGC GCC^GGC
NgoMIV 1837 GCCGGC G^CCGGC
NsiI 2959 ATGCAT ATGCA^T
Psp1406I 3758 AACGTT AA^CGTT
SacI 3105 GAGCTC GAGCT^C
ScaI 3782 AGTACT AGT^ACT
SmaI 728 CCCGGG CCC^GGG
XmaCI 728 CCCGGG C^CCGGG
XmaI 728 CCCGGG C^CCGGG
XmnI 3780 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 524 ACATGT A^C[GA][CT]GT
2318 ACGTGT
AocI 1690 CCTCAGG CC^T[ACGT]AGG
3591 CCTAAGG
Aor51HI 27 AGCGCT AGC^GCT
2927 AGCGCT
BalI 2297 TGGCCA TGG^CCA
2573 TGGCCA
BbrPI 774 CACGTG CAC^GTG
2317 CACGTG
BclI 2834 TGATCA T^GATCA
2891 TGATCA
BfaI 1267 CTAG C^TAG
3772 CTAG
BsaI 2918 GAGACC GGTCTC(1/5)
3362 GAGACC
BsmBI 1569 CGTCTC CGTCTC(1/5)
764 GAGACG
BsrFI 1116 GCCGGT [GA]^CCGG[CT]
1837 GCCGGC
BssSI 99 CACGAG CACGAG(-5/-1)
915 CTCGTG
BstXI 1940 CCACTGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2576 CCAAACTGCTGG
Bsu36I 1690 CCTCAGG CC^T[ACGT]AGG
3591 CCTAAGG
Cfr10I 1116 GCCGGT [GA]^CCGG[CT]
1837 GCCGGC
EarI 1996 CTCTTC CTCTTC(1/4)
2593 GAAGAG
Eco47III 27 AGCGCT AGC^GCT
2927 AGCGCT
Eco81I 1690 CCTCAGG CC^T[ACGT]AGG
3591 CCTAAGG
EcoNI 1620 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1686 CCTGCCTCAGG
FbaI 2834 TGATCA T^GATCA
2891 TGATCA
Ksp632I 1996 CTCTTC CTCTTC(1/4)
2593 GAAGAG
MaeI 1267 CTAG C^TAG
3772 CTAG
MluNI 2297 TGGCCA TGG^CCA
2573 TGGCCA
MscI 2297 TGGCCA TGG^CCA
2573 TGGCCA
NdeI 2741 CATATG CA^TATG
2811 CATATG
NspI 524 ACATGT [GA]CATG^[CT]
2547 GCATGT
PmaCI 774 CACGTG CAC^GTG
2317 CACGTG
PmlI 774 CACGTG CAC^GTG
2317 CACGTG
SmlI 1357 CTCAAG C^T[CT][GA]AG
2109 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AspI AvaI AvaII BamHI
BanI BanII BbeI BbsI BbvI
BcnI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV PflFI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII