##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AccI AclI AflII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I AviII AvrII BamHI
BbeI BbrPI BfrI BlnI BsaAI
BsaBI BsiWI BsmI BspDI BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI DrdI Eco47III EcoO65I FseI
FspI HincII HindII HindIII HpaI
KasI MamI NarI NdeI NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
SalI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SspI
SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 3671 GGTACC G^GTACC
AccIII 8 TCCGGA T^CCGGA
AcsI 928 GAATTC [GA]^AATT[CT]
AcyI 2574 GGCGTC G[GA]^CG[CT]C
AhdI 403 GACCGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 928 GAATTC [GA]^AATT[CT]
Asp718I 3671 GGTACC G^GTACC
AspEI 403 GACCGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 547 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 2079 GCTGAGG CCTCAGC(-5/-2)
BcgI 1826 CGAGGTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3340 GTATCC GTATCC(6/5)
BclI 2636 TGATCA T^GATCA
BsaHI 2574 GGCGTC G[GA]^CG[CT]C
BseAI 8 TCCGGA T^CCGGA
Bsp1407I 1279 TGTACA T^GTACA
BspEI 8 TCCGGA T^CCGGA
BspLU11I 2513 ACATGT A^CATGT
BsrBI 3653 CCGCTC CCGCTC(-3/-3)
BsrDI 982 CATTGC GCAATG(2/0)
BsrGI 1279 TGTACA T^GTACA
BssHII 286 GCGCGC G^CGCGC
DraI 1200 TTTAAA TTT^AAA
DraIII 2327 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 477 CGGCCG C^GGCCG
Eam1105I 403 GACCGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 477 CGGCCG C^GGCCG
Eco52I 477 CGGCCG C^GGCCG
EcoRI 928 GAATTC G^AATTC
EcoRV 3808 GATATC GAT^ATC
FbaI 2636 TGATCA T^GATCA
Hin1I 2574 GGCGTC G[GA]^CG[CT]C
KpnI 3671 GGTACC GGTAC^C
MluI 3182 ACGCGT A^CGCGT
MroI 8 TCCGGA T^CCGGA
NheI 1919 GCTAGC G^CTAGC
PaeR7I 1824 CTCGAG C^TCGAG
PflFI 547 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 567 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 246 GAAGAGC GCTCTTC(1/4)
SbfI 177 CCTGCAGG CCTGCA^GG
SphI 3266 GCATGC GCATG^C
Sse8387I 177 CCTGCAGG CCTGCA^GG
SspBI 1279 TGTACA T^GTACA
Tth111I 547 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1824 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 2513 ACATGT A^C[GA][CT]GT
3182 ACGCGT
BglII 2996 AGATCT A^GATCT
3693 AGATCT
BsaI 438 GAGACC GGTCTC(1/5)
2208 GAGACC
BsaWI 8 TCCGGA [AT]^CCGG[AT]
3590 TCCGGT
BsiEI 91 CGACCG CG[GA][CT]^CG
477 CGGCCG
BsmBI 471 GAGACG CGTCTC(1/5)
2258 GAGACG
BstAPI 488 GCAAGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3579 GCAGGAGCTGC
CpoI 401 CGGACCG CG^G[AT]CCG
455 CGGACCG
EcoT22I 2834 ATGCAT ATGCA^T
3264 ATGCAT
KspI 459 CCGCGG CCGC^GG
533 CCGCGG
MaeII 381 ACGT A^CGT
3386 ACGT
MfeI 1135 CAATTG C^AATTG
2435 CAATTG
MunI 1135 CAATTG C^AATTG
2435 CAATTG
NsiI 2834 ATGCAT ATGCA^T
3264 ATGCAT
RsrII 401 CGGACCG CG^G[AT]CCG
455 CGGACCG
SacII 459 CCGCGG CCGC^GG
533 CCGCGG
SmlI 1824 CTCGAG C^T[CT][GA]AG
3801 CTCAAG
XcmI 1243 CCAGTGCCTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2279 CCATGGGCCCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RcaI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmaCI
XmaI