##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
Acc65I AclI AgeI AocI Aor51HI
AscI Asp718I AspI BbeI BbsI
BciVI BpuAI BsiWI BspDI BsrDI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
HindIII HpaI KasI KpnI MfeI
MluI MunI NarI NdeI NheI
NotI NruI NspV PacI PflFI
PinAI PmeI PshAI Psp1406I RsrII
SalI SfiI SfoI SfuI SgrAI
SnaBI SpeI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 295 GACGTC GACGT^C
AccI 283 GTCTAC GT^[AC][GT]AC
AccIII 1475 TCCGGA T^CCGGA
AflII 205 CTTAAG C^TTAAG
AhdI 409 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2546 GTGCAC G^TGCAC
ApaLI 2546 GTGCAC G^TGCAC
AseI 2057 ATTAAT AT^TAAT
AsnI 2057 ATTAAT AT^TAAT
AspEI 409 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3139 CCTAGG C^CTAGG
BamHI 773 GGATCC G^GATCC
BbrPI 1993 CACGTG CAC^GTG
BcgI 2181 CGATACATGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 205 CTTAAG C^TTAAG
BlnI 3139 CCTAGG C^CTAGG
BsaBI 258 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1475 TCCGGA [AT]^CCGG[AT]
BseAI 1475 TCCGGA T^CCGGA
BsiEI 3923 CGATCG CG[GA][CT]^CG
BspEI 1475 TCCGGA T^CCGGA
BsrFI 4354 GCCGGC [GA]^CCGG[CT]
Cfr10I 4354 GCCGGC [GA]^CCGG[CT]
Eam1105I 409 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1064 GAATTC G^AATTC
FseI 4353 GGCCGGCC GGCCGG^CC
HincII 811 GTTGAC GT[CT]^[GA]AC
HindII 811 GTTGAC GT[CT]^[GA]AC
KspI 58 CCGCGG CCGC^GG
MamI 258 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1475 TCCGGA T^CCGGA
NaeI 4354 GCCGGC GCC^GGC
NgoMIV 4354 GCCGGC G^CCGGC
PaeR7I 424 CTCGAG C^TCGAG
PflMI 777 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1993 CACGTG CAC^GTG
PmlI 1993 CACGTG CAC^GTG
PshBI 2057 ATTAAT AT^TAAT
PvuI 3923 CGATCG CGAT^CG
SacII 58 CCGCGG CCGC^GG
ScaI 662 AGTACT AGT^ACT
SexAI 1755 ACCTGGT A^CC[AT]GGT
SspI 2133 AATATT AAT^ATT
Van91I 777 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3587 TCTAGA T^CTAGA
XhoI 424 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 127 GGCGTC G[GA]^CG[CT]C
295 GACGTC
Asp700I 2565 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3645 GAATACCTTC
AviII 215 TGCGCA TGC^GCA
1646 TGCGCA
BanI 177 GGTGCC G^G[CT][GA]CC
4202 GGCACC
BclI 842 TGATCA T^GATCA
2989 TGATCA
BglII 2916 AGATCT A^GATCT
2982 AGATCT
BsaHI 127 GGCGTC G[GA]^CG[CT]C
295 GACGTC
BsaI 1752 GAGACC GGTCTC(1/5)
2197 GGTCTC
Bsp1407I 362 TGTACA T^GTACA
941 TGTACA
BsrGI 362 TGTACA T^GTACA
941 TGTACA
BssHII 75 GCGCGC G^CGCGC
103 GCGCGC
BssSI 781 CTCGTG CACGAG(-5/-1)
2540 CTCGTG
BstAPI 2859 GCATTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3212 GCAGCACCTGC
DraI 2011 TTTAAA TTT^AAA
2601 TTTAAA
DraIII 778 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
3396 CACAATGTG
EcoNI 2414 CCTGTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3258 CCTGTCTCAGG
EcoT22I 2857 ATGCAT ATGCA^T
3090 ATGCAT
FbaI 842 TGATCA T^GATCA
2989 TGATCA
FspI 215 TGCGCA TGC^GCA
1646 TGCGCA
Hin1I 127 GGCGTC G[GA]^CG[CT]C
295 GACGTC
NcoI 1346 CCATGG C^CATGG
3032 CCATGG
NsiI 2857 ATGCAT ATGCA^T
3090 ATGCAT
SapI 2759 GAAGAGC GCTCTTC(1/4)
735 GCTCTTC
SbfI 1082 CCTGCAGG CCTGCA^GG
3945 CCTGCAGG
SphI 1669 GCATGC GCATG^C
3293 GCATGC
Sse8387I 1082 CCTGCAGG CCTGCA^GG
3945 CCTGCAGG
SspBI 362 TGTACA T^GTACA
941 TGTACA
XcmI 1482 CCAAGTAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1570 CCAACCGCAGCCTGG
XmnI 2565 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3645 GAATACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BalI BanII
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspHI BspLU11I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DrdI DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmaCI XmaI