################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## Acc65I AclI AgeI AocI Aor51HI AscI Asp718I AspI BbeI BbsI BciVI BpuAI BsiWI BspDI BsrDI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV HindIII HpaI KasI KpnI MfeI MluI MunI NarI NdeI NheI NotI NruI NspV PacI PflFI PinAI PmeI PshAI Psp1406I RsrII SalI SfiI SfoI SfuI SgrAI SnaBI SpeI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 295 GACGTC GACGT^C AccI 283 GTCTAC GT^[AC][GT]AC AccIII 1475 TCCGGA T^CCGGA AflII 205 CTTAAG C^TTAAG AhdI 409 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2546 GTGCAC G^TGCAC ApaLI 2546 GTGCAC G^TGCAC AseI 2057 ATTAAT AT^TAAT AsnI 2057 ATTAAT AT^TAAT AspEI 409 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 3139 CCTAGG C^CTAGG BamHI 773 GGATCC G^GATCC BbrPI 1993 CACGTG CAC^GTG BcgI 2181 CGATACATGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 205 CTTAAG C^TTAAG BlnI 3139 CCTAGG C^CTAGG BsaBI 258 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1475 TCCGGA [AT]^CCGG[AT] BseAI 1475 TCCGGA T^CCGGA BsiEI 3923 CGATCG CG[GA][CT]^CG BspEI 1475 TCCGGA T^CCGGA BsrFI 4354 GCCGGC [GA]^CCGG[CT] Cfr10I 4354 GCCGGC [GA]^CCGG[CT] Eam1105I 409 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 1064 GAATTC G^AATTC FseI 4353 GGCCGGCC GGCCGG^CC HincII 811 GTTGAC GT[CT]^[GA]AC HindII 811 GTTGAC GT[CT]^[GA]AC KspI 58 CCGCGG CCGC^GG MamI 258 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1475 TCCGGA T^CCGGA NaeI 4354 GCCGGC GCC^GGC NgoMIV 4354 GCCGGC G^CCGGC PaeR7I 424 CTCGAG C^TCGAG PflMI 777 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1993 CACGTG CAC^GTG PmlI 1993 CACGTG CAC^GTG PshBI 2057 ATTAAT AT^TAAT PvuI 3923 CGATCG CGAT^CG SacII 58 CCGCGG CCGC^GG ScaI 662 AGTACT AGT^ACT SexAI 1755 ACCTGGT A^CC[AT]GGT SspI 2133 AATATT AAT^ATT Van91I 777 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 3587 TCTAGA T^CTAGA XhoI 424 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcyI 127 GGCGTC G[GA]^CG[CT]C 295 GACGTC Asp700I 2565 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3645 GAATACCTTC AviII 215 TGCGCA TGC^GCA 1646 TGCGCA BanI 177 GGTGCC G^G[CT][GA]CC 4202 GGCACC BclI 842 TGATCA T^GATCA 2989 TGATCA BglII 2916 AGATCT A^GATCT 2982 AGATCT BsaHI 127 GGCGTC G[GA]^CG[CT]C 295 GACGTC BsaI 1752 GAGACC GGTCTC(1/5) 2197 GGTCTC Bsp1407I 362 TGTACA T^GTACA 941 TGTACA BsrGI 362 TGTACA T^GTACA 941 TGTACA BssHII 75 GCGCGC G^CGCGC 103 GCGCGC BssSI 781 CTCGTG CACGAG(-5/-1) 2540 CTCGTG BstAPI 2859 GCATTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3212 GCAGCACCTGC DraI 2011 TTTAAA TTT^AAA 2601 TTTAAA DraIII 778 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG 3396 CACAATGTG EcoNI 2414 CCTGTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3258 CCTGTCTCAGG EcoT22I 2857 ATGCAT ATGCA^T 3090 ATGCAT FbaI 842 TGATCA T^GATCA 2989 TGATCA FspI 215 TGCGCA TGC^GCA 1646 TGCGCA Hin1I 127 GGCGTC G[GA]^CG[CT]C 295 GACGTC NcoI 1346 CCATGG C^CATGG 3032 CCATGG NsiI 2857 ATGCAT ATGCA^T 3090 ATGCAT SapI 2759 GAAGAGC GCTCTTC(1/4) 735 GCTCTTC SbfI 1082 CCTGCAGG CCTGCA^GG 3945 CCTGCAGG SphI 1669 GCATGC GCATG^C 3293 GCATGC Sse8387I 1082 CCTGCAGG CCTGCA^GG 3945 CCTGCAGG SspBI 362 TGTACA T^GTACA 941 TGTACA XcmI 1482 CCAAGTAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1570 CCAACCGCAGCCTGG XmnI 2565 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3645 GAATACCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII BalI BanII BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspHI BspLU11I BspMI BsrBI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI