##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AclI AflII AflIII AscI AseI
AsnI AviII BbeI BcgI BfrI
BglII BsiWI BspDI BspLU11I BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI DraI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HindIII KasI
KspI MfeI MluI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PmeI PshBI
Psp1406I PvuI SacII SalI SapI
SbfI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SspI SwaI XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 2006 GACGTC GACGT^C
Acc65I 271 GGTACC G^GTACC
AccI 1514 GTCTAC GT^[AC][GT]AC
AccII 138 CGCG CG^CG
AcsI 3722 AAATTT [GA]^AATT[CT]
AgeI 2140 ACCGGT A^CCGGT
AhdI 773 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1823 GTGCAC G^TGCAC
Aor51HI 678 AGCGCT AGC^GCT
ApaLI 1823 GTGCAC G^TGCAC
ApoI 3722 AAATTT [GA]^AATT[CT]
Asp718I 271 GGTACC G^GTACC
AspEI 773 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1019 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1283 CCTAGG C^CTAGG
BamHI 1041 GGATCC G^GATCC
BbrPI 551 CACGTG CAC^GTG
BbvCI 460 GCTGAGG CCTCAGC(-5/-2)
BclI 1614 TGATCA T^GATCA
BlnI 1283 CCTAGG C^CTAGG
BlpI 412 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 412 GCTGAGC GC^T[ACGT]AGC
BsaBI 1654 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 68 CGGCCG CG[GA][CT]^CG
BsmBI 1943 CGTCTC CGTCTC(1/5)
Bsp1407I 3706 TGTACA T^GTACA
BsrGI 3706 TGTACA T^GTACA
BssSI 1763 CTCGTG CACGAG(-5/-1)
BstUI 138 CGCG CG^CG
CelII 412 GCTGAGC GC^T[ACGT]AGC
CpoI 2810 CGGACCG CG^G[AT]CCG
DrdI 1832 GACATTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 68 CGGCCG C^GGCCG
Eam1105I 773 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 68 CGGCCG C^GGCCG
Eco47III 678 AGCGCT AGC^GCT
Eco52I 68 CGGCCG C^GGCCG
FbaI 1614 TGATCA T^GATCA
HaeII 678 AGCGCT [GA]GCGC^[CT]
HpaI 2465 GTTAAC GTT^AAC
KpnI 271 GGTACC GGTAC^C
MamI 1654 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 138 CGCG CG^CG
PflFI 1019 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2140 ACCGGT A^CCGGT
PmaCI 551 CACGTG CAC^GTG
PmlI 551 CACGTG CAC^GTG
PshAI 1886 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 2810 CGGACCG CG^G[AT]CCG
ScaI 1141 AGTACT AGT^ACT
SfiI 3187 GGCCAATCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 1055 ACTAGT A^CTAGT
SphI 2520 GCATGC GCATG^C
SspBI 3706 TGTACA T^GTACA
StuI 1173 AGGCCT AGG^CCT
Tth111I 1019 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AocI 2405 CCTGAGG CC^T[ACGT]AGG
3768 CCTGAGG
ApaI 428 GGGCCC GGGCC^C
2747 GGGCCC
BalI 3186 TGGCCA TGG^CCA
3472 TGGCCA
BsaAI 551 CACGTG [CT]AC^GT[GA]
2903 TACGTG
BsaI 974 GAGACC GGTCTC(1/5)
2109 GAGACC
BsmI 2272 GAATGC GAATGC(1/-1)
3699 GCATTC
BstAPI 1812 GCATCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3820 GCACAGGATGC
Bsu36I 2405 CCTGAGG CC^T[ACGT]AGG
3768 CCTGAGG
Eco81I 2405 CCTGAGG CC^T[ACGT]AGG
3768 CCTGAGG
HincII 2465 GTTAAC GT[CT]^[GA]AC
3837 GTCAAC
HindII 2465 GTTAAC GT[CT]^[GA]AC
3837 GTCAAC
MluNI 3186 TGGCCA TGG^CCA
3472 TGGCCA
MscI 3186 TGGCCA TGG^CCA
3472 TGGCCA
PpuMI 3776 AGGACCT [GA]G^G[AT]CC[CT]
3887 AGGACCT
SexAI 1020 ACCTGGT A^CC[AT]GGT
2820 ACCTGGT
SmlI 308 CTCAAG C^T[CT][GA]AG
2867 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccIII AciI AcyI AfaI AluI
AlwI AlwNI Asp700I AspHI AvaI
AvaII BanI BanII BbsI BbvI
BciVI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MroI MseI
MslI MspA1I MspI MvaI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI