##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Aor51HI ApaI AscI AseI AsnI
AviII AvrII BamHI BfrI BlnI
BsaWI BseAI BsiWI BspEI BspMI
BsrBI BsrDI BsrFI Bst1107I BstBI
BstEII BstPI BstZ17I Cfr10I CpoI
DraII DrdI Eco47III EcoO109I EcoO65I
EcoRI EcoT22I FseI FspI HpaI
KspI MfeI MluI MroI MunI
NaeI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
Acc65I 1909 GGTACC G^GTACC
AccI 449 GTCTAC GT^[AC][GT]AC
AcyI 643 GGCGCC G[GA]^CG[CT]C
AhdI 3351 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1672 CCTCAGG CC^T[ACGT]AGG
Asp700I 2524 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1909 GGTACC G^GTACC
AspEI 3351 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2627 TGGCCA TGG^CCA
BbeI 643 GGCGCC GGCGC^C
BbrPI 3248 CACGTG CAC^GTG
BbsI 350 GAAGAC GAAGAC(2/6)
BcgI 3 CGATTCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2109 GGATAC GTATCC(6/5)
BclI 2835 TGATCA T^GATCA
BglI 1478 GCCGAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2771 AGATCT A^GATCT
BlpI 1780 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1780 GCTCAGC GC^T[ACGT]AGC
BpuAI 350 GAAGAC GAAGAC(2/6)
BsaAI 3248 CACGTG [CT]AC^GT[GA]
BsaBI 2126 GATAGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 643 GGCGCC G[GA]^CG[CT]C
BsgI 3520 CTGCAC GTGCAG(16/14)
BspDI 1903 ATCGAT AT^CGAT
BssSI 3523 CACGAG CACGAG(-5/-1)
BstAPI 1153 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 2771 AGATCT [GA]^GATC[CT]
Bsu36I 1672 CCTCAGG CC^T[ACGT]AGG
CelII 1780 GCTCAGC GC^T[ACGT]AGC
ClaI 1903 ATCGAT AT^CGAT
Eam1105I 3351 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1848 GAAGAG CTCTTC(1/4)
Eco81I 1672 CCTCAGG CC^T[ACGT]AGG
EcoNI 1790 CCTATGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2072 GATATC GAT^ATC
FbaI 2835 TGATCA T^GATCA
Hin1I 643 GGCGCC G[GA]^CG[CT]C
HincII 2098 GTTGAC GT[CT]^[GA]AC
HindII 2098 GTTGAC GT[CT]^[GA]AC
HindIII 3470 AAGCTT A^AGCTT
KasI 643 GGCGCC G^GCGCC
KpnI 1909 GGTACC GGTAC^C
Ksp632I 1848 GAAGAG CTCTTC(1/4)
MaeII 3249 ACGT A^CGT
MamI 2126 GATAGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 2771 AGATCT [GA]^GATC[CT]
MluNI 2627 TGGCCA TGG^CCA
MscI 2627 TGGCCA TGG^CCA
MspA1I 2993 CAGCTG C[AC]G^C[GT]G
NarI 643 GGCGCC GG^CGCC
NdeI 2500 CATATG CA^TATG
PaeR7I 3343 CTCGAG C^TCGAG
PmaCI 3248 CACGTG CAC^GTG
PmlI 3248 CACGTG CAC^GTG
PvuII 2993 CAGCTG CAG^CTG
SacI 2607 GAGCTC GAGCT^C
SfoI 643 GGCGCC GGC^GCC
XbaI 2520 TCTAGA T^CTAGA
XhoI 3343 CTCGAG C^TCGAG
XhoII 2771 AGATCT [GA]^GATC[CT]
XmnI 2524 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwNI 1154 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
2258 CAGGTGCTG
AspI 904 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1273 GACTCCGTC
AvaI 1296 CTCGGG C^[CT]CG[GA]G
3343 CTCGAG
BbvCI 147 CCTCAGC CCTCAGC(-5/-2)
1441 GCTGAGG
BsmI 1939 GAATGC GAATGC(1/-1)
1234 GCATTC
BsoBI 1296 CTCGGG C^[CT]CG[GA]G
3343 CTCGAG
Bsp1407I 2195 TGTACA T^GTACA
3402 TGTACA
BspLU11I 2178 ACATGT A^CATGT
2744 ACATGT
BsrGI 2195 TGTACA T^GTACA
3402 TGTACA
BssHII 1410 GCGCGC G^CGCGC
1412 GCGCGC
BstXI 157 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1899 CCACATCGATGG
DraI 1748 TTTAAA TTT^AAA
3602 TTTAAA
DraIII 1259 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
3332 CACAGAGTG
DsaI 1528 CCGTGG C^C[GA][CT]GG
1553 CCGTGG
PflFI 904 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1273 GACTCCGTC
PflMI 265 CCAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1094 CCAGACGATGG
ScaI 2596 AGTACT AGT^ACT
2643 AGTACT
SfcI 2957 CTATAG C^T[GA][CT]AG
3490 CTACAG
SmlI 2104 CTTGAG C^T[CT][GA]AG
3343 CTCGAG
SspBI 2195 TGTACA T^GTACA
3402 TGTACA
Tth111I 904 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1273 GACTCCGTC
Van91I 265 CCAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1094 CCAGACGATGG
XcmI 535 CCATTGTCATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3301 CCAGAGTTTGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI ApaLI ApoI
AspHI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BspHI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EagI EclXI Eco52I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I