##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII Alw44I ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AviII BbrPI BcgI BclI
BfrI BsmI BspDI BspHI BspLU11I
BsrDI Bst1107I BstAPI BstBI BstZ17I
ClaI DraI DraIII DrdI EcoRI
EcoRV EcoT22I FbaI FspI HincII
HindII HpaI KpnI MfeI MluI
MseI MunI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI SalI SbfI
ScaI SfuI SnaBI SpeI Sse8387I
SspI SwaI Tru9I Tsp509I Tth111I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AccIII 1184 TCCGGA T^CCGGA
AvrII 3488 CCTAGG C^CTAGG
BciVI 1146 GGATAC GTATCC(6/5)
BglII 3025 AGATCT A^GATCT
BlnI 3488 CCTAGG C^CTAGG
BlpI 1505 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1505 GCTCAGC GC^T[ACGT]AGC
BsaAI 2455 CACGTA [CT]AC^GT[GA]
BsaBI 3026 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1188 GAGACC GGTCTC(1/5)
BseAI 1184 TCCGGA T^CCGGA
BsiWI 2356 CGTACG C^GTACG
BsmBI 2037 GAGACG CGTCTC(1/5)
Bsp1407I 3110 TGTACA T^GTACA
BspEI 1184 TCCGGA T^CCGGA
BsrGI 3110 TGTACA T^GTACA
CelII 1505 GCTCAGC GC^T[ACGT]AGC
CpoI 3061 CGGTCCG CG^G[AT]CCG
FseI 2420 GGCCGGCC GGCCGG^CC
HindIII 3451 AAGCTT A^AGCTT
MamI 3026 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1184 TCCGGA T^CCGGA
NdeI 183 CATATG CA^TATG
RsrII 3061 CGGTCCG CG^G[AT]CCG
SacI 1463 GAGCTC GAGCT^C
SapI 100 GAAGAGC GCTCTTC(1/4)
SphI 1609 GCATGC GCATG^C
SspBI 3110 TGTACA T^GTACA
XcmI 1133 CCAGGGCCGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AgeI 1257 ACCGGT A^CCGGT
3059 ACCGGT
AhdI 1090 GACAGTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3313 GACGCTTTGTC
AocI 336 CCTGAGG CC^T[ACGT]AGG
2835 CCTAAGG
Aor51HI 2134 AGCGCT AGC^GCT
2908 AGCGCT
AspEI 1090 GACAGTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3313 GACGCTTTGTC
AspHI 1463 GAGCTC G[AT]GC[AT]^C
2547 GAGCAC
BfaI 619 CTAG C^TAG
3489 CTAG
BsiHKAI 1463 GAGCTC G[AT]GC[AT]^C
2547 GAGCAC
BsrBI 1298 GAGCGG CCGCTC(-3/-3)
1334 GAGCGG
BssSI 1815 CACGAG CACGAG(-5/-1)
3204 CTCGTG
BstEII 126 GGTTACC G^GT[ACGT]ACC
1248 GGTTACC
BstPI 126 GGTTACC G^GT[ACGT]ACC
1248 GGTTACC
Bsu36I 336 CCTGAGG CC^T[ACGT]AGG
2835 CCTAAGG
Eam1105I 1090 GACAGTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3313 GACGCTTTGTC
Eco47III 2134 AGCGCT AGC^GCT
2908 AGCGCT
Eco81I 336 CCTGAGG CC^T[ACGT]AGG
2835 CCTAAGG
EcoO65I 126 GGTTACC G^GT[ACGT]ACC
1248 GGTTACC
KspI 3090 CCGCGG CCGC^GG
3425 CCGCGG
MaeI 619 CTAG C^TAG
3489 CTAG
MaeII 190 ACGT A^CGT
2456 ACGT
NspI 1069 GCATGT [GA]CATG^[CT]
1609 GCATGC
PinAI 1257 ACCGGT A^CCGGT
3059 ACCGGT
PvuII 1527 CAGCTG CAG^CTG
2816 CAGCTG
SacII 3090 CCGCGG CCGC^GG
3425 CCGCGG
SexAI 634 ACCTGGT A^CC[AT]GGT
3304 ACCTGGT
SgrAI 1256 CACCGGTG C[GA]^CCGG[CT]G
1912 CGCCGGTG
StuI 235 AGGCCT AGG^CCT
2192 AGGCCT
TfiI 2049 GATTC G^A[AT]TC
3473 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AvaI AvaII
BalI BamHI BanI BanII BbeI
BbsI BbvCI BbvI BcnI BglI
BmyI BpmI BpuAI BsaHI BsaJI
BsaWI BseRI BsgI BsiEI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssHII BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV PflMI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfiI
SfoI SmaI SmlI StyI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XhoII XmaCI XmaI