################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AccIII AclI AflII Aor51HI AscI AseI AsnI AspI BbeI BciVI BfrI BlpI Bpu1102I BseAI BsiWI BspDI BspEI BssHII Bst1107I BstBI BstZ17I CelII ClaI CpoI DraI DraIII Eco47III EcoNI EcoRI EcoRV EcoT22I FseI HincII HindII HpaI KasI KspI MfeI MluI MroI MunI NaeI NarI NgoMIV NotI NsiI NspV PacI PaeR7I PflFI PmeI PshBI Psp1406I PvuI RsrII SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SspI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AatII 4500 GACGTC GACGT^C Acc65I 514 GGTACC G^GTACC AhdI 4119 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp718I 514 GGTACC G^GTACC AspEI 4119 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 3989 TGCGCA TGC^GCA AvrII 980 CCTAGG C^CTAGG BamHI 4262 GGATCC G^GATCC BclI 3550 TGATCA T^GATCA BglII 2182 AGATCT A^GATCT BlnI 980 CCTAGG C^CTAGG BsaBI 2523 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 2378 CGGCCG CG[GA][CT]^CG BsmI 2265 GAATGC GAATGC(1/-1) BspHI 2167 TCATGA T^CATGA BssSI 819 CACGAG CACGAG(-5/-1) BstAPI 3806 GCAAACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DrdI 357 GACGGCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 2378 CGGCCG C^GGCCG Eam1105I 4119 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 2378 CGGCCG C^GGCCG Eco52I 2378 CGGCCG C^GGCCG FbaI 3550 TGATCA T^GATCA FspI 3989 TGCGCA TGC^GCA HindIII 2696 AAGCTT A^AGCTT KpnI 514 GGTACC GGTAC^C MamI 2523 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NdeI 1363 CATATG CA^TATG NheI 3331 GCTAGC G^CTAGC NruI 3579 TCGCGA TCG^CGA RcaI 2167 TCATGA T^CATGA SapI 1688 GCTCTTC GCTCTTC(1/4) TaqI 2179 TCGA T^CGA TthHB8I 2179 TCGA T^CGA XbaI 3387 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 3195 GTAGAC GT^[AC][GT]AC 3255 GTAGAC AcsI 2685 AAATTC [GA]^AATT[CT] 3500 GAATTT AgeI 432 ACCGGT A^CCGGT 2447 ACCGGT AocI 1300 CCTCAGG CC^T[ACGT]AGG 3839 CCTGAGG ApoI 2685 AAATTC [GA]^AATT[CT] 3500 GAATTT Asp700I 2066 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2171 GAAGAGGTTC BbrPI 1409 CACGTG CAC^GTG 2577 CACGTG BglI 1008 GCCAGCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1385 GCCTCAGCGGC BsaWI 432 ACCGGT [AT]^CCGG[AT] 2447 ACCGGT Bsp1407I 2951 TGTACA T^GTACA 4402 TGTACA BspLU11I 763 ACATGT A^CATGT 1543 ACATGT BsrDI 2667 GCAATG GCAATG(2/0) 3242 GCAATG BsrGI 2951 TGTACA T^GTACA 4402 TGTACA Bsu36I 1300 CCTCAGG CC^T[ACGT]AGG 3839 CCTGAGG Eco81I 1300 CCTCAGG CC^T[ACGT]AGG 3839 CCTGAGG MseI 703 TTAA T^TAA 4549 TTAA PinAI 432 ACCGGT A^CCGGT 2447 ACCGGT PmaCI 1409 CACGTG CAC^GTG 2577 CACGTG PmlI 1409 CACGTG CAC^GTG 2577 CACGTG SacI 1138 GAGCTC GAGCT^C 2647 GAGCTC SbfI 3041 CCTGCAGG CCTGCA^GG 3812 CCTGCAGG SphI 859 GCATGC GCATG^C 4395 GCATGC Sse8387I 3041 CCTGCAGG CCTGCA^GG 3812 CCTGCAGG SspBI 2951 TGTACA T^GTACA 4402 TGTACA Tru9I 703 TTAA T^TAA 4549 TTAA XmnI 2066 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2171 GAAGAGGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI ApaI ApaLI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcgI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PshAI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TfiI TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII