##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AccIII AclI AflII Aor51HI AscI
AseI AsnI AspI BbeI BciVI
BfrI BlpI Bpu1102I BseAI BsiWI
BspDI BspEI BssHII Bst1107I BstBI
BstZ17I CelII ClaI CpoI DraI
DraIII Eco47III EcoNI EcoRI EcoRV
EcoT22I FseI HincII HindII HpaI
KasI KspI MfeI MluI MroI
MunI NaeI NarI NgoMIV NotI
NsiI NspV PacI PaeR7I PflFI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SspI SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 4500 GACGTC GACGT^C
Acc65I 514 GGTACC G^GTACC
AhdI 4119 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 514 GGTACC G^GTACC
AspEI 4119 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3989 TGCGCA TGC^GCA
AvrII 980 CCTAGG C^CTAGG
BamHI 4262 GGATCC G^GATCC
BclI 3550 TGATCA T^GATCA
BglII 2182 AGATCT A^GATCT
BlnI 980 CCTAGG C^CTAGG
BsaBI 2523 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 2378 CGGCCG CG[GA][CT]^CG
BsmI 2265 GAATGC GAATGC(1/-1)
BspHI 2167 TCATGA T^CATGA
BssSI 819 CACGAG CACGAG(-5/-1)
BstAPI 3806 GCAAACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 357 GACGGCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2378 CGGCCG C^GGCCG
Eam1105I 4119 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2378 CGGCCG C^GGCCG
Eco52I 2378 CGGCCG C^GGCCG
FbaI 3550 TGATCA T^GATCA
FspI 3989 TGCGCA TGC^GCA
HindIII 2696 AAGCTT A^AGCTT
KpnI 514 GGTACC GGTAC^C
MamI 2523 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 1363 CATATG CA^TATG
NheI 3331 GCTAGC G^CTAGC
NruI 3579 TCGCGA TCG^CGA
RcaI 2167 TCATGA T^CATGA
SapI 1688 GCTCTTC GCTCTTC(1/4)
TaqI 2179 TCGA T^CGA
TthHB8I 2179 TCGA T^CGA
XbaI 3387 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 3195 GTAGAC GT^[AC][GT]AC
3255 GTAGAC
AcsI 2685 AAATTC [GA]^AATT[CT]
3500 GAATTT
AgeI 432 ACCGGT A^CCGGT
2447 ACCGGT
AocI 1300 CCTCAGG CC^T[ACGT]AGG
3839 CCTGAGG
ApoI 2685 AAATTC [GA]^AATT[CT]
3500 GAATTT
Asp700I 2066 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2171 GAAGAGGTTC
BbrPI 1409 CACGTG CAC^GTG
2577 CACGTG
BglI 1008 GCCAGCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1385 GCCTCAGCGGC
BsaWI 432 ACCGGT [AT]^CCGG[AT]
2447 ACCGGT
Bsp1407I 2951 TGTACA T^GTACA
4402 TGTACA
BspLU11I 763 ACATGT A^CATGT
1543 ACATGT
BsrDI 2667 GCAATG GCAATG(2/0)
3242 GCAATG
BsrGI 2951 TGTACA T^GTACA
4402 TGTACA
Bsu36I 1300 CCTCAGG CC^T[ACGT]AGG
3839 CCTGAGG
Eco81I 1300 CCTCAGG CC^T[ACGT]AGG
3839 CCTGAGG
MseI 703 TTAA T^TAA
4549 TTAA
PinAI 432 ACCGGT A^CCGGT
2447 ACCGGT
PmaCI 1409 CACGTG CAC^GTG
2577 CACGTG
PmlI 1409 CACGTG CAC^GTG
2577 CACGTG
SacI 1138 GAGCTC GAGCT^C
2647 GAGCTC
SbfI 3041 CCTGCAGG CCTGCA^GG
3812 CCTGCAGG
SphI 859 GCATGC GCATG^C
4395 GCATGC
Sse8387I 3041 CCTGCAGG CCTGCA^GG
3812 CCTGCAGG
SspBI 2951 TGTACA T^GTACA
4402 TGTACA
Tru9I 703 TTAA T^TAA
4549 TTAA
XmnI 2066 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2171 GAAGAGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcgI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PshAI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TfiI TseI Tsp45I
Tsp509I TspRI Van91I XcmI XhoII